Rhodiola linearifolia Boriss., a perennial alpine plant from the Crassulaceae family, is renowned for its unique medicinal properties. However, existing research on this species is limited, particularly regarding the impact of altitude on its physiological and medicinal compounds. The current study employed morphophysiological and anatomical methods to explore the adaptive mechanisms of R. linearifolia across different altitudinal gradients, while also examining photosynthetic pigments and metabolomic changes. Our results indicate that despite the simultaneous effects of various mountain abiotic factors, significant correlations can be identified between altitude and trait variation. An optimal growth altitude of 2687 m above sea level was identified, which is pivotal for sustainable ecosystem management and potential species introduction strategies. It is noted that increasing altitude stress enhances the synthesis of secondary antioxidant metabolites in R. linearifolia, enhancing its pharmaceutical potential.

The current study investigated the impact of cold stress on the morphological, physiological, and phytochemical properties of Juglans regia L. (J. regia) using in vitro microclone cultures. The study revealed significant stress-induced changes in the production of secondary antioxidant metabolites. According to gas chromatography–mass spectrometry (GC–MS) analyses, the stress conditions profoundly altered the metabolism of J. regia microclones. Although the overall spectrum of metabolites was reduced, the production of key secondary antioxidant metabolites significantly increased. Notably, there was a sevenfold (7×) increase in juglone concentration. These findings are crucial for advancing walnut metabolomics and enhancing our understanding of plant responses to abiotic stress factors. Additionally, study results aid in identifying the role of individual metabolites in these processes, which is essential for developing strategies to improve plant resilience and tolerance to adverse conditions.

In this article, for the first time, an experimental study of the effect of mild and moderate osmotic stress, NaCl content and the effect of low positive temperature on photosynthetic activity and composition of metabolites of immature plants Sedum hybridum L. is reported. In this representative of the genus Sedum adapted to arid conditions and having the properties of a succulent, a change in photosynthetic activity and an increase in the level of protective metabolites in the shoots were revealed when exposed to mild and moderate stress factors. The results of this study can be used in work on the adaptation of succulent plants to arid conditions, environmental monitoring and work on the directed induction of valuable secondary metabolites in succulents to obtain new herbal medicines.

Globally, gall-forming insects significantly contribute to the degradation of desert ecosystems. Recent studies have demonstrated that Haloxylon persicum suffers less damage from gall-formers compared to Haloxylon aphyllum. However, the mechanisms driving the long-term metabolic responses of these species to gall-forming biotic stress in their natural environment remain unclear. The current study comparatively analyzes the anatomical features and metabolomic changes in H. aphyllum and H. persicum damaged by gall-forming insects. This research aimed to uncover potential metabolic tolerance mechanisms through GC-MS analysis. The study findings indicate that gall-forming insects cause a reduction in nearly all the anatomical structures of Haloxylon shoots, with the effects being less severe in H. persicum than in H. aphyllum. Thus, the metabolic pathways responsible for the biosynthesis of biologically active substances that enhance resistance to gall inducers were different, specifically in H. aphyllum—the biosynthesis of fatty acids (+their derivatives) and γ-tocopherol (vitamin E) and H. persicum—the biosynthesis of fatty acids (+their derivatives), dialkyl ethers, carbohydrates (+their derivatives), aromatic acid derivatives, phytosterols, γ-tocopherol (vitamin E), phenols, and terpenoids. The results suggest that the modulation of metabolic pathways under biotic stress plays a crucial role in the enhanced survival and growth of H. persicum.

Bread is a global dietary staple, but its susceptibility to fungal spoilage causes substantial food waste, economic losses, and greenhouse gas emissions. Volatile organic compounds (VOCs) produced by sourdough microorganisms can contribute to the prevention of bread spoilage; however, the specific VOCs that exert the protective effect in the bread matrix have not been precisely identified. Our study investigated the antifungal activity of sourdough lactic acid bacteria (LAB) consortia, both alone and in combination with yeast and acetic acid bacteria (AAB), as well as the VOC profiles of sourdough and sourdough bread. The consortium comprising Lactiplantibacillus plantarum 9-5 and Levilactobacillus brevis 9-2 (LAB), Monosporozyma unispora R3SD1d (yeast), and Acetobacter fabarum WB and Komagataeibacter rhaeticus Ch2 (AAB) extended the mold-free shelf life of sourdough bread by 8–20 days after challenge tests. VOC analysis revealed that sourdough bread had significantly higher concentrations of ethyl esters of hexanoic and octanoic acids with 6.3- to 9.7-fold increase in peak areas after three days of refrigerated storage, assuming their decisive role in prevention of fungal spoilage. In conclusion, the use of this consortium is promising to significantly extend bread shelf life, but also to enrich the bread VOC profile.