This paper characterizes non-indigenous fish species (NIS) and analyses both atmospheric and sea surface temperatures for the Mediterranean coast of Egypt from 1991 to 2020, in relation to previous reports in the same areas. Taxonomical characterization depicts 47 NIS from the Suez Canal (Lessepsian/alien) and 5 from the Atlantic provenance. GenBank accession number of the NIS mitochondrial gene, cytochrome oxidase 1, reproductive and commercial biodata, and a schematic Inkscape drawing for the most harmful Lessepsian species were reported. For sea surface temperatures (SST), an increase of 1.2 °C to 1.6 °C was observed using GIS software. The lack of linear correlation between annual air temperature and annual SST at the same detection points (Pearson r) could suggest a difference in submarine currents, whereas the Pettitt homogeneity test highlights a temperature breakpoint in 2005–2006 that may have favoured the settlement of non-indigenous fauna in the coastal sites of Damiette, El Arish, El Hammam, Alexandria, El Alamain, and Mersa Matruh, while there seems to be a breakpoint present in 2001 for El Sallum. This assessment of climate trends is in good agreement with the previous sightings of non-native fish species. New insights into the assessment of Egyptian coastal climate change are discussed.

Antifoulant paints were developed to prevent and reduce biofouling on surfaces immersed in seawater. The widespread use of these substances over the years has led to a significant increase of their presence in the marine environment. These compounds were identified as environmental and human threats. As a result of an international ban, research in the last decade has focused on developing a new generation of benign antifoulant paints. This review outlines the detrimental effects associated with biocide versus eco-friendly antifoulants, highlighting what are effective antifoulants and why there is a need to monitor them. We examine the effects of biocide and eco-friendly antifoulants on the antioxidative defence mechanism and settlement in a higher sessile organism, specifically the Mediterranean mussel, Mytilus galloprovincialis. These antifoulants can indirectly assess the potential of these two parameters in order to outline implementation of sustainable antifoulants.

Trichomonas vaginalis is the causative agent of one of the most widespread sexually transmitted diseases in the world. The adhesion of the parasite to the vaginal epithelial cells is mediated by specific proteins and by a complex glycan structure, the lipoglycan (TvLG), which covers the pathogen surface. L-rhamnose is an important component of TvLG, comprising up to 40% of the monosaccharides. Thus, the inhibition of its production could lead to a severe alteration in the TvLG structure, making the L-rhamnose biosynthetic pathway an attractive pharmacologic target. We report the identification and characterization of the first committed and limiting step of the L-rhamnose biosynthetic pathway, UDP-D-glucose 4,6-dehydratase (UGD, EC 4.2.1.76). The enzyme shows a strong preference for UDP-D-glucose compared to dTDP-D-glucose; we propose that the mechanism underlying the higher affinity for the UDP-bound substrate is mediated by the differential recognition of ribose versus the deoxyribose of the nucleotide moiety. The identification of the enzymes responsible for the following steps of the L-rhamnose pathway (epimerization and reduction) was more elusive. However, sequence analyses suggest that in T. vaginalis L-rhamnose synthesis proceeds through a mechanism different from the typical eukaryotic pathways, displaying intermediate features between the eukaryotic and prokaryotic pathways and involving separate enzymes for the epimerase and reductase activities, as observed in bacteria. Altogether, these results form the basis for a better understanding of the formation of the complex glycan structures on TvLG and the possible use of L-rhamnose biosynthetic enzymes for the development of selective inhibitors.

Catel–Manzke syndrome (CMS) is a rare genetic disorder associated with mutations in the TDP‐glucose 4,6‐dehydratase ( TGDS ) gene, the function of which in vertebrates remains unclear. This study investigated the zebrafish ortholog tgds to assess its suitability for modeling the disease. During development, the tgds transcript exhibits a conserved biphasic expression pattern with an initial maternal contribution followed by a second wave of expression after gastrulation. Recombinant zebrafish Tgds expressed in Escherichia coli demonstrated UDP‐D‐glucose 4,6‐dehydratase ( EC4.2.1.76 ) activity, similar to TGDS orthologs in lower eukaryotes, where it acts as the first step in the L‐rhamnose biosynthetic pathway. This finding suggests the presence of a yet unidentified pathway in vertebrates. Furthermore, CMS‐associated mutations in conserved residues significantly impair enzyme activity and stability. CRISPR/Cas9‐mediated F ₀ knockout of tgds resulted in a range of developmental defects in zebrafish. In particular, craniofacial cartilage alterations, associated with a decrease in sulfate glycosaminoglycan content, mirrored some skeletal features observed in humans with CMS. These findings establish the zebrafish as a relevant model to further explore CMS pathogenesis and the in vivo function of tgds .