Alan Buddie

Dr. Alan Buddie


Research Focus & Metrics

Academic Career Length

27Years (2000-Present)

(Calculated once Scilit publication data is synced: current year − year of first publication + 1)

Representative Publications

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22 June 2017 in Scientific Reports
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Journal Article
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MCMatthew Cock
Matthew Cock
PBPatrick K. Beseh
Patrick K. Beseh
ABAlan Buddie
Alan Buddie
GCGiovanni Cafá
Giovanni Cafá
JCJayne Crozier
Jayne Crozier
Spodoptera frugiperda (J. E. Smith) (Lepidoptera: Noctuidae) is a polyphagous pest indigenous throughout the Americas, which recently appeared in Africa, first reported from São Tomé, Nigeria, Bénin and Togo in 2016, and which we now report from Ghana. This species is recognised to comprise two morphologically identical but genetically distinct strains or species in the Americas, and we found both to be present in Ghana. We discuss possible routes of entry to Africa, of which the likeliest is adults and/or egg masses transported on direct commercial flights between the Americas and West Africa, followed by dispersal by adult flight within Africa. Identification of Lepidoptera is normally based on the markings and morphology of adults, and not on the larvae which actually cause the damage, and therefore larvae have to be reared through to adult for authoritative identification. We confirmed that the use of DNA barcoding allowed unequivocal identification of this new pest from Ghana based on the larvae alone. As authenticated barcodes for vouchered specimens of more pests become available, this approach has the potential to become a valuable in-country tool to support national capability in rapid and reliable pest diagnosis and identification.

29 March 2019 in Insects
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MKMarc Kenis
Marc Kenis
HPHannalene Du Plessis
Hannalene Du Plessis
JBJohnnie Van den Berg
Johnnie Van den Berg
MBMalick Niango Ba
Malick Niango Ba
GGGeorg Goergen
Georg Goergen
Andrew PolaszekAndrew Polaszek
Andrew Polaszek
The fall armyworm, Spodoptera frugiperda, a moth originating from tropical and subtropical America, has recently become a serious pest of cereals in sub-Saharan Africa. Biological control offers an economically and environmentally safer alternative to synthetic insecticides that are being used for the management of this pest. Consequently, various biological control options are being considered, including the introduction of Telenomus remus, the main egg parasitoid of S. frugiperda in the Americas, where it is already used in augmentative biological control programmes. During surveys in South, West, and East Africa, parasitized egg masses of S. frugiperda were collected, and the emerged parasitoids were identified through morphological observations and molecular analyses as T. remus. The presence of T. remus in Africa in at least five countries provides a great opportunity to develop augmentative biological control methods and register the parasitoid against S. frugiperda. Surveys should be carried out throughout Africa to assess the present distribution of T. remus on the continent, and the parasitoid could be re-distributed in the regions where it is absent, following national and international regulations. Classical biological control should focus on the importation of larval parasitoids from the Americas.

21 January 2020 in Insects
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LALakpo Koku Agboyi
Lakpo Koku Agboyi
GGGeorg Goergen
Georg Goergen
PBPatrick K. BESEH
Patrick K. BESEH
Samuel MensahSamuel Mensah
Samuel Mensah
VCVICTOR ATTUQUAYE CLOTTEY
VICTOR ATTUQUAYE CLOTTEY
MKMarc Kenis
Marc Kenis
The fall armyworm, Spodoptera frugiperda, a moth originating from the American continent, has recently invaded most African countries, where it is seriously threatening food security as a pest of cereals. The current management methods rely heavily on the use of synthetic insecticides but there is a need for more sustainable control methods, including biological control. Surveys were conducted in two West African countries, Ghana and Benin, to determine the native parasitoid complex and assess parasitism rates of S. frugiperda. Samples of S. frugiperda eggs and larvae were collected in maize fields located in 56 and 90 localities of Ghana and Benin, respectively, from July 2018 to July 2019. Ten species were found parasitizing the pest, including two egg parasitoids, one egg–larval, five larval and two larval–pupal parasitoids. The two most abundant parasitoids in both countries were two Braconidae: the egg-larval parasitoid Chelonus bifoveolatus and the larval parasitoid Coccygidum luteum. Parasitism rates were determined in three Ghanaian regions and averages varied from 0% to 75% between sites and from 5% to 38% between regions. These data provide an important baseline for the development of various biological control options. The two egg parasitoids, Telenomus remus and Trichogramma sp. can be used in augmentative biological control and investigations should be conducted to assess how cultural practices can enhance the action of the main parasitoids, C. luteum and Ch. bifoveolatus, in the field. Understanding the parasitoid complex of S. frugiperda in Africa is also necessary before any development of classical biological controls involving the introduction of parasitoids from the Americas.

25 March 2021 in CABI Agriculture and Bioscience
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Journal Article
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ABAlan Buddie
Alan Buddie
IRIvan Rwomushana
Ivan Rwomushana
LOLisa C. Offord
Lisa C. Offord
SKSimeon Kibet
Simeon Kibet
FMFernadis Makale
Fernadis Makale
RDRoger Day
Roger Day
Following reports of an invasive snail causing crop damage in the expansive Mwea irrigation scheme in Kenya, samples of snails and associated egg masses were collected and sent to CABI laboratories in the UK for molecular identification. DNA barcoding analyses using the cytochrome oxidase subunit I gene gave preliminary identification of the snails as Pomacea canaliculata, widely considered to have the potential to be one of the most invasive invertebrates of waterways and irrigation systems worldwide and which is already causing issues throughout much of south-east Asia. To the best of our knowledge, this is the first documented record of P. canaliculata in Kenya, and the first confirmed record of an established population in continental Africa. This timely identification shows the benefit of molecular identification and the need for robust species identifications: even a curated sequence database such as that provided by the Barcoding of Life Data system may require additional checks on the veracity of the underlying identifications. We found that the egg mass tested gave an identical barcode sequence to the adult snails, allowing identifications to be made more rapidly. Part of the nuclear elongation factor 1 alpha gene was sequenced to confirm that the snail was P. canaliculata and not a P. canaliculata/P. maculata interspecies hybrid. Given the impact of this species in Asia, there is need for an assessment of the risk to Africa, and the implementation of an appropriate response in Kenya and elsewhere to manage this new threat to agriculture and the environment.

11 August 2025 in Fungal Biology Reviews
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Journal Article
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JAJoris Alkemade
Joris Alkemade
NHNichola J. Hawkins
Nichola J. Hawkins
EBElena Baraldi
Elena Baraldi
ABAlan Buddie
Alan Buddie
HCHelen Cockerton
Helen Cockerton
TBTimothy G. Barraclough
Timothy G. Barraclough

2 July 2019 in World Journal of Microbiology and Biotechnology
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Journal Article
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DSDavid Smith
David Smith
ABAlan Buddie
Alan Buddie
RGRebecca Goss
Rebecca Goss
JOJörg Overmann
Jörg Overmann
CLC. Lepleux
C. Lepleux
MPM. Pina
M. Pina
Marine microbial diversity offers enormous potential for discovery of compounds of crucial importance in healthcare, food security and bioindustry. However, access to it has been hampered by the difficulty of accessing and growing the organisms for study. The discovery and exploitation of marine bioproducts for research and commercial development requires state-of-the-art technologies and innovative approaches. Technologies and approaches are advancing rapidly and keeping pace is expensive and time consuming. There is a pressing need for clear guidance that will allow researchers to operate in a way that enables the optimal return on their efforts whilst being fully compliant with the current regulatory framework. One major initiative launched to achieve this, has been the advent of European Research Infrastructures. Research Infrastructures (RI) and associated centres of excellence currently build harmonized multidisciplinary workflows that support academic and private sector users. The European Marine Biological Research Infrastructure Cluster (EMBRIC) has brought together six such RIs in a European project to promote the blue bio-economy. The overarching objective is to develop coherent chains of high-quality services for access to biological, analytical and data resources providing improvements in the throughput and efficiency of workflows for discovery of novel marine products. In order to test the efficiency of this prototype pipeline for discovery, 248 rarely-grown organisms were isolated and analysed, some extracts demonstrated interesting biochemical properties and are currently undergoing further analysis. EMBRIC has established an overarching and operational structure to facilitate the integration of the multidisciplinary value chains of services to access such resources whilst enabling critical mass to focus on problem resolution.