The implementation of natural parks in cities is a current and controversial theme. Therefore, in Lake Buritis, which is a leisure area for the population of the city of Goiatuba, Goiás, Brazil, the quality of the water was analyzed, carrying out quantitative tests of the water samples at six different points between the source and the outlet of the lake that flows into the stream, Chico À Toa. Physical–chemical tests (turbidity, pH, alkalinity, electrical conductivity, color, nitrate and hardness parameters), microbiological tests for species identification, analysis of the antimicrobial susceptibility profile, metals analysis and Allium cepa test, were performed. The total coliforms number in water samples was higher than the maximum value established by Brazilian legislation, demonstrating high fecal contamination during the spring in samples from an artesian well. The bacterial diversity found was large and there were many pathogenic bacteria. The A. cepa test demonstrated a cytotoxic potential for water from the source and outlet of Buritis Lake. Statistical tests were applied to verify existing correlations between parameters. Among the analyzed data, the highest correlation was between the color and turbidity parameters and the grouping between the metals (lead, iron, cadmium and magnesium).

Background: Recurrent tonsillitis is one of the most common diseases in childhood, caused many times by ß-lactam-resistant S. aureus. The objective of this study was to investigate an alternative method to identify resistance to oxacillin/cefoxitin in S. aureus from hospitalized children with recurrent tonsillitis. Methods: The samples of S. aureus came from patients with recurrent tonsillitis and were used in 16S rRNA sequencing and an antibiogram test for identification and verifying resistance, after which HSI methodology were applied for separation of S. aureus resistances. Results: The S. aureus isolated showed sensitivity to oxacillin/cefoxitin and the diagnostic images show a visual description of the resistance different groups formed, that may be related to sensitivity and resistance to oxacillin/cefoxitin, characterizing the MRSA S. aureus. Conclusions: Samples that showed phenotypic resistance to oxacillin/cefoxitin were clearly separated from samples that did not show this resistance. A PLS-DA model predicted the presence of resistance to oxacillin/cefoxitin in S. aureus samples and it was possible to observe the pixels classified as MRSA. The HSI was able to successfully discriminate samples in replicas that were sensitive and resistant, based on the calibration model it received.

Water is an important asset for the maintenance of life and socioeconomic development. Aquatic environments have been anthropized, receiving a large polluting load, mainly from trace elements.This study investigates the occurrence of health risks caused by 15 trace elements in the surface water of João Leite stream. Health risk indices were evaluated as average daily dose (ADD), hazard quotient (HQ), hazard index and carcinogenic risk. The risk analysis to human health of the stream João Leite water, indicated that the ADDingestion for the adults had the highest for Fe 1.86E-00 µg/kg/day and lower value for Be 8.0E-05 µg/kg/day. On the other hand, of ADDdermal the highest value was for Fe 5.02E-02 µg/kg/day and, the smallest for Sb 1.54E-05 µg/kg/day. For children to ADDingestion obtained was obtained the highest Fe value 2.60E-00 µg/kg/day and smaller for Be 1.12E-04 µg/kg/day. For ADDdermal the highest value was for Fe 8.58E-02 µg/kg/day and smaller for Sb 2.64E-05 µg/kg/day. HQ for adults ranged from 1.61E-05 to 2.97E-01 for HQingestion and 7.71E-06 to 1.01E-01 for HQdermal. For children 2.25E-05 to 3.74E-01 for HQingestion and 1.32E-05 to 1.73E-01 for HQdermal. The health risk assessment showed that for children, the trace elements present in water have a potentially adverse effect on non-carcinogenic health. The carcinogenic risks were unacceptable for both children and adults. Thus, it is recommended that the use of these waters be limited and that measures be taken to minimize pollution by trace elements.

The behavior of insects on the corpse, as well as colonizing species, can cause dismemberment, burial, exposure of body parts and even the generation of artifacts. This study aimed to show the need for forensic entomology as a criminal tool, highlighting its investigative importance for determining the cause of death and mainly evaluating the estimated time elapsed between the date of death and the finding of the body. This is an integrative literature review, of the descriptive-exploratory type, regarding the use of forensic entomology as a tool to determine the Post-Mortem Interval (PMI). Essential parameters were observed so that the maximum and minimum time could be determined when it comes to the analysis of the life stage of insects and arthropods found under the body. Among the research, it was possible to verify that several factors can alter the colonization of insects, such as where the body is located, climatic aspects and its regional location, in addition there are artifacts left by the insects themselves that, if not properly analyzed, can lead to erroneous hypotheses.

The genus Chromobacterium spp. are gram-negative bacilli, which may or may not have characteristic purple pigmentation, and are mainly isolated from soil, water and infected patients. The main representative species of this genus is C. violaceum, responsible for a high mortality rate among those infected. The aim of this study was to genetically characterize and broadly compare phenotypic characteristics between the genus Chromobacterium species described in the literatureand bacterial isolates. This study is an experimental research, in which sequencing and phenotypic tests of bacterial isolates were carried out for comparison with species of the genus Chromobacterium. Two strains were identified, CRJL01 and CRJL02 that have characteristics of Chromobacterium spp species. These isolates showed high resistance to antibiotics, tolerance and resistance to metals, biochemical and physiological versatility of the CRJL01 and CRJL02 strains. In the sequencing of the 16S rRNA gene, the CRJL01 strain showed similarity with the C. piscinae strain. The CRJL02 strain showed similarity with the C. subtsugae strain. This work is the first report in 40 years of Chromobacterium spp. in the Brazilian Midwest – Goiás, in water samples. This isolated genus has a wide applicability for the pharmaceutical, food and cosmetic industries, due to the production of its purple/violet pigment known as violacein, and its bioprospecting is of great importance. Thus, this study is a kick-off for exploring your produced pigment.