This study aimed to identify the critical factors and items affecting the productivity of sustainable human resources in a Railway Operation Company based on the perceptions of employees and managers in the Human Resources Department. The study was motivated by research which was applied in terms of the objectives of the study and a descriptive survey was employed as the method. The statistical population of the current study consisted of all employees and managers of the Human Resources Department of the company. Random sampling was employed to collect data and the sample size was 191 people according to Morgan’s Table. Methods including the correlation coefficient, multivariate regression, and factor analysis were employed for data analysis. The findings highlight the main factors and items affecting labor productivity in the Urban and Suburban Railway Operation Company as perceived by the Human Resources Department, which were mainly related to human resources management and could be attributed to motivation and requirements for their effective contribution to the improvement of public welfare. Organizational Attitude and Culture, Leadership Style, and Bonus and Ergonomics were extracted as factors affecting productivity or as independent variables. This study is the first study that has aimed to discuss the perceptions of the Human Resources Department active in a company. As such, the study highlights the standpoint of the main decision makers in the Urban and Suburban Railway Operation Company with regard to labour productivity in the urban and suburban sector.