Background. Noodle products are popular throughout the world, and they can be prepared from cereal like wheat, maize, and rice. Nowadays, healthy and nutritious product requirement has been increasing. Thus, research on the nutrition-rich but neglected crop is becoming visible nowadays to ensure global food security and to satisfy the nutritional need. Research indicated that moringa tree leaf powder has good nutritional value, but it is not yet customized and properly consumed. Method. The study is aimed at developing noodles from wheat flour and moringa leaf powder and evaluating proximate composition, antinutritional content (phytate and tannin), microbial load (total plate count and yeast and mold count), and sensory acceptability. The experiment contains four treatments and one control. The data from proximate composition, antinutritional content, microbial load, and sensory acceptability were subjected to SAS version 9 software. A complete randomized design was used to analyze the proximate composition, antinutritional content, and microbial load data, and a randomized complete block design was used to analyze the acceptability test. Result. The study revealed that in the noodles formulated from 80 durum wheat flour and 20 of moringa leaf powder, the ash, protein, fat, fiber, gross energy, phytate, and tannin content were increased by 39.39, 10.86, 153, 42.2, 3.43, 39.83, and 329.78, respectively, as compared with noodles made from 100 durum wheat flour. However, moisture, total bacteria count, and yeast and mold count were decreased by 28.71, 45.52, and 55.93, respectively. Similarly, the study also revealed that the acceptability test of noodles was decreased as moringa leaf powder concentration is increased. Conclusion. In conclusion, besides the good nutritional profile and antimicrobial capacity, moringa has antinutritional content and influences the sensory acceptability of products. Therefore, limiting the moringa leaf powder concentration is needed during the development of products using moringa leaf powder.

In southern Ethiopian households, kocho is one of the staple foods which can be kept longer and fermented naturally using locally prepared pits, but evidence about the influences of fermentation of kocho at a different time and agroecology on proximate compositions and microbial loads are limited. Fermented kocho samples at different fermentation times were collected from highland and midland districts of Sidama region of Ethiopia. The standard procedure of AOAC (2005) method was followed. Four microbiological load analyses were conducted. Factorial analysis using JMP 13 was conducted. Across the fermentation time, total carbohydrate, ash, crude protein, and crude fat ranged 36%–40%, 1.9%–3.2%, 3%–4.3%, and 0.1%–0.3%, respectively. The highest total ash content was observed in week one of fermentation both in midland and highland samples. However, in midland, the increment of fermentation time showed a reduction of total ash percentage. Crude protein and fat were observed similar both in midland and highland (p > .05). The titrable acidity of Kocho varied from 0.16% to 0.22%. It was shown that it increased in the first three months of fermentation. It was also found to be increased as the fermentation time is increasing. Aerobic mesophilic, lactic acid bacteria, yeast, and mold were highly observed in Kocho as compared to Enterobacteriaceae. The loads varied across the fermentation time. Enterobacteriaceae and yeast and mold count of Kocho decreased with increased fermentation time. In conclusion, agroecology did not affect crude protein percentage as the fermentation time is increased. However, it was shown that fermentation increases protein and fat percentages. The increment of the acidic contents may also suppress the microbial growth for better food safety of kocho products.

Fried food consumption is popular in most parts of the world including Ethiopia. Among many fried products available in Ethiopia, fried fish is most commonly consumed in Hawassa Town due to the easy access to the fish from the lake. Recently, there is growing concern among fryers to recycle the oil while frying fish. However, there is limited evidence about the frying effect on the fatty-acid-related health lipid index of fried fish. Thus, the study was aimed to determine the fatty acid profile and the fatty-acid-related health lipid of raw and fried fish. Raw and fried fish were taken from the Hawassa open fish market. Fatty acid profiles were analyzed using a gas chromatography-mass spectrophotometer (GCMS), and the health lipid index was determined by calculation using the recommended formula. JMP pro 13 version software was used for data analysis. Our result showed that raw fish had a high amount of essential fatty acid, nutritive value index, hypocholesterolemic ratio, and peroxidizability index. In contrary to this, the fried fish had a high amount of trans-fatty acids, nonessential fatty acids, atherogenic index, and thrombogenic index. In conclusion, the fried fish loses its fatty-acid-related nutritional quality in uncontrolled frying conditions. Therefore, frying needs to be controlled, as it risks human health otherwise.

Proximate, Antinutritional, Microbial, and Sensory Acceptability of Bread Formulated from Wheat (Triticum aestivum) and Amaranth (Amaranthus caudatus): Background. Breads are made throughout the world. Bread can be prepared from cereal like wheat, maize, and rice. Nowadays, gluten intolerance, requirement of healthy, and nutritious products have increased and interests towards underutilized crops have also been increasing with the aim of improving global food security and to ease an adverse effect of climate changes. Amaranth is one of nutritionally balanced and naturally grown underutilized crops, but it is mainly considered weed in Africa including Ethiopia. Method. The aim of the study is to develop bread from wheat and Amaranthus and to evaluate proximate composition, antinutritional, microbial, and sensory acceptability of bread. The experiment contained 100 wheat as control and four blending proportions (90 wheat and 10 amaranth, 80 wheat and 20 amaranth, 70 wheat and 30 amaranth, and 60 wheat and 40 amaranth). A complete randomized design is used for proximate composition, antinutritional, and microbial data analysis whereas a randomized complete block design with three replications was applied for sensory acceptability. SAS for windows version 9 was used for data analysis. Result. The study revealed that moisture, protein, fat, fiber, and antinutritional content were increased as Amaranthus concentration is increased from 10 to 40. However, carbohydrate, microbial load, and sensory acceptability were decreased. But the gross energy is constant. Conclusion. From the study, it can be concluded that beside the good nutritional profile of Amaranthus, it has antinutritional content which needs to limit the concentration of Amaranthus in blending with other grains during product development.

Fish constitutes a occasional food for the Sidama people of Hawassa, the capital city of Ethiopia’s Sidama Region and the site of a large endorheic lake. Freshly-caught fish, especially Nile tilapia or koroso in Local name, are typically fried prior to consumption. Despite the sensory qualities, fried foods are not always advisable due to the impact of frying on the nutritional quality of food. This study was designed to assess the nutritional quality of Nile tilapia that had been fried in the same palm oil over six consecutive frying cycles. The raw fish were purchased from the Lake Hawassa fish market and fried at Hawassa University by simulating local preparation methods. A gas chromatography-mass spectrophotometer (GCMS) was used for the fatty acid profile analysis and a total of 22 fatty acids were elucidated. The nutritional quality indices of fatty acids was determined by calculating the recommended formula and JMP pro 13 was used for statistical analysis. The study results revealed that the tilapia fried in the oldest, most used oil, cycles 3–6, contained high amounts of saturated and trans fatty acids, as well as high atherogenic and thrombogenic indices; however, it was also lower in essential and cis fatty acids, the hypocholesterolemic/hypercholesterolemic ratio, the per-oxidizability index, and the nutritive value index. Conversely, fish prepared earlier (cycles 1–3) with fresher oil were higher in essential fatty acids and cis fatty acids, while the hypocholesterolemic/hypercholesterolemic ratio, the per-oxidizability index, and the nutritive value index were also high. Fish fried in an earlier cycle were also low in saturated and trans-fatty acids, with a lower atherogenic index, and thrombogenic index. Therefore, it can be concluded that repeatedly using the same frying oil to prepare Nile tilapia contributed to the loss of nutritional value. Results suggest that palm oil should be limited to no more than three frying cycles to maximize nutritional intake in of fish consumption.

Fresh water fish is considered a source of good quality proteins and essential fats. Frying is among widely used fish preparation techniques globally. Deep-frying oil quality with repeated uses, has been a concern. This research investigated the influence of frying oil stability (expressed as levels of peroxide values (0.2, 1.2 meq/kg) and free fatty acids (0.05, 0.13% palmitic acid)) over repeated uses (1,6 cycles) on the oxidative stability and essential nutrients of fried fish using a response surface approach, with the objectives of generating information relevant for the improvement of community health outcomes, with a special focus of this particular research was on trends, but not optimization. The frying oil and fried fish quality were adequately explained by response surface model and supported by principal component analysis. The result showed that the oxidative stability of both the frying oil and fried fish were deteriorating over the reuse cycles of the frying oil (with increasing trends of unhealthy fats, saturated and trans) corresponding to decreasing trends in the healthy fat components and vitamin A). It was also indicated that the essential fats (omega 3, omega 6, cis, vitamin A), were decreased while the risky fats (saturated, trans) were increased along with the frying cycles. The result revealed the urgent need for regulating frying oil and fried food qualities, particularly in developing countries.