Alcohol consumption is a major public health problem facing universities. The objectives of the present study were to investigate alcohol consumption and the behaviors associated with it among Romanian university medical students, with particular reference to sex differences, behavioral consequences, and lifestyle patterns. We performed a cross-sectional study on 722 medical students (60.4% females; 39.6% males); the participants filled out a validated questionnaire containing the following items co-occurring with alcohol consumption: smoking, illicit drug use, energy drink consumption, and other behavioral drinking consequences. Physical activity was estimated using the IPAQ questionnaire. We statistically analyzed the interrelation between alcohol consumption and target factors. The present study showed a high percentage of at-risk drinkers among male (15.0%) and female medical students (14.9%) in the studied group. Male students reported higher illicit drug use and physical activity than female students, but the at-risk female drinkers’ group consumed more drugs than the low-risk female drinkers. Both male and female drinkers engaged in other risky behaviors correlated with drinking (e.g., smoking, low academic performance, and driving a car after drinking). Public health policies, strategies, and interventions should be initiated to reduce alcohol consumption and associated behaviors in medical students.

Background and Objectives: The ongoing COVID-19 pandemic has posed an array of new challenges for medical students worldwide. Amidst academic rigors, students are confronted with unique stressors, potentially affecting their mental health and substance use. This study aimed to investigate the multifaceted effects of depression, alcohol use, and stress on medical students and discern how these factors have been amplified by the pandemic’s circumstances, and to identify predictors of mental distress during the COVID-19 pandemic period. Materials and Methods: Two online anonymous and cross-sectional surveys were conducted at the University of Medicine and Pharmacy “Iuliu Hatieganu” in Romania among medical students in 2018 and in 2022. Data were gathered via online questionnaires distributed through closed student groups on social media platforms, with a total of 1061 participants, to investigate stress, depression, alcohol and drug use, and the impact of the pandemic and online education on mental health, maintaining anonymity and ethical approval. The Medical Student Stress Questionnaire (MSSQ) was employed to measure different aspects of stress, the Beck Depression Inventory (BDI) provided insights into the participants’ depressive symptoms, and for the assessment of alcohol consumption habits, the Alcohol Use Disorders Identification Test (AUDIT) was utilized. Results: Our findings showed a significant decrease in mean depression scores (13.81 vs. 11.56, with p < 0.001) from 2018 to 2022. In 2018, students scored significantly higher in the overall stress-related domains. Additionally, being female, facing financial constraints, and being in pre-clinical years emerged as predictors of heightened academic-related stress and depressive symptoms. Students who had experienced the loss of a family member due to COVID-19 exhibited a statistically significant rise in their average BDI score and current anxiety levels. Conclusions: The pandemic, despite introducing new stressors, may have indirectly fostered an increased focus on students’ mental health, leading to more refined support mechanisms. Specialized interventions, taking into account gender and financial problems, are needed to address the multifaceted challenges faced by medical students. Our study highlights the ongoing need to nurture both the academic and emotional strength of future medical practitioners.

Pregnancy is a transformative stage in a woman’s life, marked by significant physical, emotional, and social changes. This study had three main objectives: (1) to assess the quality of life (QoL) of pregnant women in Romania, (2) to identify the sociodemographic, health, and obstetric factors influencing their QoL and (3) to examine the psychometric properties of the WHOQOL-BREF questionnaire within the Romanian context, determining its effectiveness in evaluating QoL during pregnancy. Methods: A cross-sectional analytical survey was conducted between January and July 2023 among pregnant women in Romania, targeting a geographically diverse sample from urban and rural areas. Eligible participants were Romanian citizens aged 18 or older. Data were collected through an online self-administered questionnaire using Google Forms, with informed consent obtained electronically. The survey included demographic, obstetric, and health-related variables alongside the WHOQOL-BREF tool, which evaluates QoL across four dimensions: Physical, Psychological, Social Relationships, and Environment. Statistical analysis involved confirmatory factor analysis, reliability testing (Cronbach’s α and McDonald’s ω), and comparisons using Welch’s t-tests and ANOVA. Results: A total of 1550 valid responses were analyzed. The WHOQOL-BREF demonstrated excellent internal consistency (Cronbach’s α > 0.9 across all dimensions). Women with higher education and stable employment reported significantly higher QoL scores in physical and psychological dimensions. No significant differences were found based on pregnancy trimester, previous births, or participation in prenatal classes, although trends suggested slight advantages for participants in prenatal education. Women delivering in private hospitals or non-hospital settings reported better psychological and physical QoL than those delivering in public hospitals. Support from partners and urban residency positively influenced perceived QoL. Conclusions: The WHOQOL-BREF is a reliable tool for assessing QoL in pregnant women in Romania. The study highlights the role of education, employment, and delivery location in influencing QoL, emphasizing the need for targeted support for vulnerable groups during pregnancy.

Crisis situations, which cause disturbances in the daily routine of the individual can contribute to the development of mental problems. On the 26^th of February, 2020, the first confirmed case of COVID-19 was reported in Romania. On the 16^th of March, the President of Romania declared The State of Emergency. The following day, by Order of the Ministry of the Internal Affairs medical students starting with the 4^th year of study were to be recruited, on a voluntary basis, to support activities in the Emergency Rooms, if needed. Numerous medical students volunteered. The medical students were exposed to online learning and the possibility of being recruited as volunteers in COVID-19 units. In April 2020, we created and distributed an online survey via www.psysafe.org, which collected data on demographics, personal status of infection, and general mental health, including medication and substance use, sleeping problems in the previous week, perceived stress in the previous month, and burnout in the previous two months. We translated and adapted 2 questionnaires to assess the COVID-19 related anxiety and fear. The participants were Romanian medical students. Data was statistically analysed with SPSS 20. Out of 550 surveys filled, we analysed 545 valid surveys. The mean age of the respondents was 21.66 (±2.55) years, with 81.7% female (445) and 18.3% male (100). 91.6% of the respondents declared that they were never diagnosed with a psychiatric disorder. Coffee was used on a daily basis by 53.8% of the students, with tobacco coming in second in 19.4%. Female students perceived a higher level of stress and showed a higher level of burnout. Female students scored higher for the anxiety and fear related to Covid-19. Medical students with mental health history scored higher on perceived stress, burnout, anxiety and fear related to COVID-19. Perceived stress indicated a level of moderate stress regardless of the mental history status. For all students, the burnout value indicated that some attention was needed, as they might had been candidates to burnout. Moreover, students diagnosed with a psychiatric disorder during the pandemic showed signs that they were on the road to burnout, with changes needed to be done at the time. There were statistically significant positive correlation, of various degrees between perceived stress, burnout, anxiety and fear related to COVID-19, with the strongest one observed for the perceived stress and burnout, in Romanian medical students.

Mushrooms have long been recognized as a rich source of bioactive compounds, including phenolics, that possess important antioxidant, antimicrobial, and antibacterial properties, including activity against drug-resistant bacteria. This study evaluated total phenolic profile and content, total flavonoids content, the antioxidant activities, antimicrobial and antibacterial activities, of water extracts of edible mushrooms from Romanian deciduous forests, including Cantharellus cibarius, Russula virescens, Lactarius piperatus, and Boletus edulis. The extracts were characterized using high-performance liquid chromatography coupled with mass spectrometry (HPLC-MS) and Fourier Transform Infrared Spectroscopy (FTIR) analysis. Antioxidant activity was determined using DPPH radical-scavenging activity and ABTS radical cation decolorization assay. Antimicrobial and antibacterial activities were tested using standard strains of Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Enterococcus faecalis, and Streptococcus pneumoniae following diffusion testing and time-killing assay, respectively. The HPLC-MS results indicated that major compounds in all the mushrooms belonged to the subclass of hydroxybenzoic acids. Trans-cinnamic acid and hydroxybenzoic acids, particularly gallic acid, 2,3-dihydroxybenzoic acid, and gentisic acid, were the predominant compounds detected in BEE and CCE. Their concentrations were measured as follows: 24 μg/mL, 63 μg/mL, 56 μg/mL, and 14 μg/mL, respectively, for BEE, and 26 μg/mL, 42 μg/mL, 7 μg/mL, and 5 μg/mL, respectively, for CCE. Among phenolic compounds, 2-dihydroxybenzoic acid, 2,3-dihydroxybenzoic acid, p-anisaldehyde, and gentisic acid were positively correlated with both DPPH (45% and 21% inhibition rate for BEE and CCE, respectively) and ABTS (64 and 31% inhibition rate for BEE and CCE, respectively) antioxidant activities. The FTIR analysis revealed the presence of lipids, proteins, and polysaccharides, extracted in different ratios in the water extract. All mushroom extracts showed a dose-dependent response with higher antimicrobial and antibacterial activities at the highest concentration (26.3 µg phenolics BEE, 12.7 µg pphenolics CCE, 28.3 µg phenolics LPE, and 14.5 µg phenolics RVE per well for antimicrobial activity and 175.2 µg phenolics/mL BEE, 84.4 µg phenolics/mL CCE, and 188.9 µg phenolics/mL LPE for antibacterial activity). These species demonstrate potential for the development of alternative antimicrobial formulations, particularly relevant in the context of antibiotic resistance.