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Peoples' Friendship University of Russia (RUDN University) 117198, Moscow Miklukho-Maklaya str. 6
Lymphopenia is a frequent hematological manifestation, associated with a severe course of COVID-19, with an insufficiently understood pathogenesis. We present molecular genetic immunohistochemical, and electron microscopic data on SARS-CoV-2 dissemination and viral load (VL) in lungs, mediastinum lymph nodes, and the spleen of 36 patients who died from COVID-19. Lymphopenia <1 × 109/L was observed in 23 of 36 (63.8%) patients. In 12 of 36 cases (33%) SARS-CoV-2 was found in lung tissues only with a median VL of 239 copies (range 18–1952) SARS-CoV-2 cDNA per 100 copies of ABL1. Histomorphological changes corresponding to bronchopneumonia and the proliferative phase of DAD were observed in these cases. SARS-CoV-2 dissemination into the lungs, lymph nodes, and spleen was detected in 23 of 36 patients (58.4%) and was associated with the exudative phase of DAD in most of these cases. The median VL in the lungs was 12,116 copies (range 810–250281), lymph nodes—832 copies (range 96–11586), and spleen—71.5 copies (range 0–2899). SARS-CoV-2 in all cases belonged to the 19A strain. A immunohistochemical study revealed SARS-CoV-2 proteins in pneumocytes, alveolar macrophages, and bronchiolar epithelial cells in lung tissue, sinus histiocytes of lymph nodes, as well as cells of the Billroth pulp cords and spleen capsule. SARS-CoV-2 particles were detected by transmission electron microscopy in the cytoplasm of the endothelial cell, macrophages, and lymphocytes. The infection of lymphocytes with SARS-CoV-2 that we discovered for the first time may indicate a possible link between lymphopenia and SARS-CoV-2-mediated cytotoxic effect.
Adhamjon Abdullaev; Akmaljon Odilov; Maxim Ershler; Alexey Volkov; Tatiana Lipina; Tatiana Gasanova; Yuri Lebedin; Igor Babichenko; Andrey Sudarikov. Viral Load and Patterns of SARS-CoV-2 Dissemination to the Lungs, Mediastinal Lymph Nodes, and Spleen of Patients with COVID-19 Associated Lymphopenia. Viruses 2021, 13, 1410 .
AMA StyleAdhamjon Abdullaev, Akmaljon Odilov, Maxim Ershler, Alexey Volkov, Tatiana Lipina, Tatiana Gasanova, Yuri Lebedin, Igor Babichenko, Andrey Sudarikov. Viral Load and Patterns of SARS-CoV-2 Dissemination to the Lungs, Mediastinal Lymph Nodes, and Spleen of Patients with COVID-19 Associated Lymphopenia. Viruses. 2021; 13 (7):1410.
Chicago/Turabian StyleAdhamjon Abdullaev; Akmaljon Odilov; Maxim Ershler; Alexey Volkov; Tatiana Lipina; Tatiana Gasanova; Yuri Lebedin; Igor Babichenko; Andrey Sudarikov. 2021. "Viral Load and Patterns of SARS-CoV-2 Dissemination to the Lungs, Mediastinal Lymph Nodes, and Spleen of Patients with COVID-19 Associated Lymphopenia." Viruses 13, no. 7: 1410.
The aim of the study was to evaluate the histopathological changes in the lungs of patients who died of a new coronavirus infection (COVID-19) in relation to the length of hospital stay. We evaluated lung autopsy material, autopsy reports, and death summaries of 39 patients who died of COVID-19. The length of hospital stay ranged from a few hours to 25 days. At all stages of the disease, lung alterations (desquamation of bronchial and alveolar epithelium), circulatory disorders (alveolar edema and hemorrhages, congestion in small blood vessels, thrombosis), compensatory response (fibrosis) were identified. The patients who died during the first week of hospitalization demonstrated predominant signs of circulatory disorders (alveolar edema, hyaline membranes, alveolar hemorrhages, congestion in small blood vessels). Fibrosis, usually not typical for the first week of acute respiratory distress syndrome, was detected in 46% of the deceased during the first week of hospitalization, which may be due to late hospitalization or patterns of fibrosis development in COVID-19. For those who died in the 2nd and 3rd weeks of hospitalization, the compensatory response and progression of fibrosis were noted. By the 3rd week, pulmonary fibrosis was detected in 91% of patients. Thrombotic complications (thrombosis, pulmonary artery thromboembolism) were observed in almost half of fatalities occurring during weeks 2–3. Hemorrhagic infarction was found in 43% (6 patients) who died during week 2 of hospitalization, three of them were diagnosed with pulmonary embolism, indicating progression of pulmonary vascular damage.
Anastasia Babkina; Arkady Golubev; Alexey Volkov; Artem Kuzovlev; Maryam Khadzhieva; Oleg Grebenchikov; Lev Golubev. CLINICAL AND HISTOPATHOLOGICAL FEATURES OFLUNG INJURY IN COVID-19 INFECT. Archiv Euromedica 2021, 11, 15 -19.
AMA StyleAnastasia Babkina, Arkady Golubev, Alexey Volkov, Artem Kuzovlev, Maryam Khadzhieva, Oleg Grebenchikov, Lev Golubev. CLINICAL AND HISTOPATHOLOGICAL FEATURES OFLUNG INJURY IN COVID-19 INFECT. Archiv Euromedica. 2021; 11 (1):15-19.
Chicago/Turabian StyleAnastasia Babkina; Arkady Golubev; Alexey Volkov; Artem Kuzovlev; Maryam Khadzhieva; Oleg Grebenchikov; Lev Golubev. 2021. "CLINICAL AND HISTOPATHOLOGICAL FEATURES OFLUNG INJURY IN COVID-19 INFECT." Archiv Euromedica 11, no. 1: 15-19.
In 2020 the world faced the pandemic of COVID-19 severe acute respiratory syndrome caused by a new type of coronavirus named SARS-CoV-2. To stop the spread of the disease, it is crucial to create molecular tools allowing the investigation, diagnoses and treatment of COVID-19. One of such tools are monoclonal antibodies (mAbs). In this study we describe the development of hybridoma cells that can produce mouse mAbs against receptor binding domain of SARS-CoV-2 spike (S) protein. These mAbs are able to specifically detect native and denatured S proteins in all tested applications, including immunoblotting, enzyme-linked immunosorbent assay, immunofluorescence staining of cells and immunohistochemical staining of paraffin embedded patients’ tissue samples. In addition, we showed that the obtained mAbs can efficiently block SARS-CoV-2 infection in in vitro experiments. Finally, we determined the amino acid sequence of light and heavy chains of the mAbs. This information will allow the use of corresponding peptides to establish genetically engineered therapeutic antibodies. To date multiple mAbs against SARS-CoV-2 proteins have been established, however, bigger sets of various antibodies will allow the detection and neutralization of SARS-CoV-2, even if the virus acquires novel mutations.
Nadezhda V. Antipova; Tatyana D. Larionova; Andrei E. Siniavin; Maria A. Nikiforova; Vladimir A. Gushchin; Igor I. Babichenko; Alexey V. Volkov; Michail I. Shakhparonov; Marat S. Pavlyukov. Establishment of Murine Hybridoma Cells Producing Antibodies against Spike Protein of SARS-CoV-2. International Journal of Molecular Sciences 2020, 21, 9167 .
AMA StyleNadezhda V. Antipova, Tatyana D. Larionova, Andrei E. Siniavin, Maria A. Nikiforova, Vladimir A. Gushchin, Igor I. Babichenko, Alexey V. Volkov, Michail I. Shakhparonov, Marat S. Pavlyukov. Establishment of Murine Hybridoma Cells Producing Antibodies against Spike Protein of SARS-CoV-2. International Journal of Molecular Sciences. 2020; 21 (23):9167.
Chicago/Turabian StyleNadezhda V. Antipova; Tatyana D. Larionova; Andrei E. Siniavin; Maria A. Nikiforova; Vladimir A. Gushchin; Igor I. Babichenko; Alexey V. Volkov; Michail I. Shakhparonov; Marat S. Pavlyukov. 2020. "Establishment of Murine Hybridoma Cells Producing Antibodies against Spike Protein of SARS-CoV-2." International Journal of Molecular Sciences 21, no. 23: 9167.
Medical article медицинская статья
Yu. I. Pigolkin; M. P. Poletaeva; G. V. Zolotenkova; Alexey Volkov. The age-specific changes in the histological structure of the thyroid cartilage in the men. Sudebno-meditsinskaya ekspertiza 2017, 60, 11 -14.
AMA StyleYu. I. Pigolkin, M. P. Poletaeva, G. V. Zolotenkova, Alexey Volkov. The age-specific changes in the histological structure of the thyroid cartilage in the men. Sudebno-meditsinskaya ekspertiza. 2017; 60 (5):11-14.
Chicago/Turabian StyleYu. I. Pigolkin; M. P. Poletaeva; G. V. Zolotenkova; Alexey Volkov. 2017. "The age-specific changes in the histological structure of the thyroid cartilage in the men." Sudebno-meditsinskaya ekspertiza 60, no. 5: 11-14.
A. A. Muraev; A.P. Bonartsev; Y. Gazhva; V.M. Riabova; Alexey Volkov; Irina Zharkova; I.A. Stamboliev; E.S. Kuznetsova; V.A. Zhuikov; V.L. Myshkina; T.K. Mahina; Garina Bonartseva; Sergey Yakovlev; Ksenia Kudryashova; V.V. Voinova; A.A. Mironov; K.V. Shaitan; S.I. Gazhva; S. Yu. Ivanov. Development and Preclinical Studies of Orthotopic Bone Implants Based on a Hybrid Construction from Poly(3-Hydroxybutyrate) and Sodium Alginate. Sovremennye tehnologii v medicine 2016, 8, 42 -50.
AMA StyleA. A. Muraev, A.P. Bonartsev, Y. Gazhva, V.M. Riabova, Alexey Volkov, Irina Zharkova, I.A. Stamboliev, E.S. Kuznetsova, V.A. Zhuikov, V.L. Myshkina, T.K. Mahina, Garina Bonartseva, Sergey Yakovlev, Ksenia Kudryashova, V.V. Voinova, A.A. Mironov, K.V. Shaitan, S.I. Gazhva, S. Yu. Ivanov. Development and Preclinical Studies of Orthotopic Bone Implants Based on a Hybrid Construction from Poly(3-Hydroxybutyrate) and Sodium Alginate. Sovremennye tehnologii v medicine. 2016; 8 (4):42-50.
Chicago/Turabian StyleA. A. Muraev; A.P. Bonartsev; Y. Gazhva; V.M. Riabova; Alexey Volkov; Irina Zharkova; I.A. Stamboliev; E.S. Kuznetsova; V.A. Zhuikov; V.L. Myshkina; T.K. Mahina; Garina Bonartseva; Sergey Yakovlev; Ksenia Kudryashova; V.V. Voinova; A.A. Mironov; K.V. Shaitan; S.I. Gazhva; S. Yu. Ivanov. 2016. "Development and Preclinical Studies of Orthotopic Bone Implants Based on a Hybrid Construction from Poly(3-Hydroxybutyrate) and Sodium Alginate." Sovremennye tehnologii v medicine 8, no. 4: 42-50.
A.L. Zernov; E.A. Ivanov; T.K. Makhina; V.L. Myshkina; O.V. Samsonova; A.V. Feofanov; Alexey Volkov; Y. Gazhva; A. A. Muraev; Valentina Mikhailovna Ryabova; K.V. Shaitan; S. Yu. Ivanov; Garina Bonartseva; Anton Bonartsev. Microcapsules of Poly(3-Hydroxybutyrate) for Prolonged Protein Release. Sovremennye tehnologii v medicine 2015, 7, 50 -57.
AMA StyleA.L. Zernov, E.A. Ivanov, T.K. Makhina, V.L. Myshkina, O.V. Samsonova, A.V. Feofanov, Alexey Volkov, Y. Gazhva, A. A. Muraev, Valentina Mikhailovna Ryabova, K.V. Shaitan, S. Yu. Ivanov, Garina Bonartseva, Anton Bonartsev. Microcapsules of Poly(3-Hydroxybutyrate) for Prolonged Protein Release. Sovremennye tehnologii v medicine. 2015; 7 (4):50-57.
Chicago/Turabian StyleA.L. Zernov; E.A. Ivanov; T.K. Makhina; V.L. Myshkina; O.V. Samsonova; A.V. Feofanov; Alexey Volkov; Y. Gazhva; A. A. Muraev; Valentina Mikhailovna Ryabova; K.V. Shaitan; S. Yu. Ivanov; Garina Bonartseva; Anton Bonartsev. 2015. "Microcapsules of Poly(3-Hydroxybutyrate) for Prolonged Protein Release." Sovremennye tehnologii v medicine 7, no. 4: 50-57.
We determined conditions for effective transduction of multipotent mesenchymal stromal cells from human adipose tissue with adenoviral constructs carrying the gene of human bone morphogenetic protein BMP-2. The peak of transgene transcription and BMP-2 protein secretion in the transduced cultures was observed on day 6 after infection. The maximum transcription of BMP-2 gene and genes of osteogenic markers (bone sialoprotein, osteopontin, and osteocalcin) was observed in the medium containing sodium β-glycerophosphate and ascorbic acid. Addition of D 3 vitamin did not enhance the expression of BMP-2 gene in transduced cells. The obtained cell cultures with high osteogenic potential can be used in bone tissue engineering.
T. B. Bukharova; L. V. Logovskaya; A. V. Volkov; M. N. Garas; E. B. Vikhrova; D. Yu. Logunov; O. V. Makhnach; Maxim Shmarov; D. V. Gol’Dshtein. Adenoviral transduction of multipotent mesenchymal stromal cells from human adipose tissue with bone morphogenetic protein BMP-2 gene. Bulletin of Experimental Biology and Medicine 2013, 156, 122 -126.
AMA StyleT. B. Bukharova, L. V. Logovskaya, A. V. Volkov, M. N. Garas, E. B. Vikhrova, D. Yu. Logunov, O. V. Makhnach, Maxim Shmarov, D. V. Gol’Dshtein. Adenoviral transduction of multipotent mesenchymal stromal cells from human adipose tissue with bone morphogenetic protein BMP-2 gene. Bulletin of Experimental Biology and Medicine. 2013; 156 (1):122-126.
Chicago/Turabian StyleT. B. Bukharova; L. V. Logovskaya; A. V. Volkov; M. N. Garas; E. B. Vikhrova; D. Yu. Logunov; O. V. Makhnach; Maxim Shmarov; D. V. Gol’Dshtein. 2013. "Adenoviral transduction of multipotent mesenchymal stromal cells from human adipose tissue with bone morphogenetic protein BMP-2 gene." Bulletin of Experimental Biology and Medicine 156, no. 1: 122-126.
We studied of osteogenic differentiation of multipotent mesenchymal stromal cells from human adipose tissue. Experiments showed that 1α,25-dihydroxycalciferol is a more effective inductor of osteogenesis than dexamethasone. Comparative analysis revealed activation of gene expression for the major osteogenic markers on day 7 of culturing in a medium containing 1α,25-dihydroxycalciferol. It was found that transcription of genes encoding type 1 collagen proteins, osteopontin, osteocalcin, and bone sialoprotein peaked on day 14 in culture, while the expression of alkaline phosphatase and bone morphogenetic protein-2 genes increased over 21 days. Intensive mineralization of the extracellular matrix was observed starting from day 14 in culture. On the basis of the analysis of these data, optimal terms for osteogenic induction (day 14) and an optimal inductor (1α,25-dihydroxycalciferol) were chosen and the protocol of effective osteogenic differentiation of multipotent mesenchymal stromal cells from human adipose tissue was developed for creation of tissue-engineered bone equivalents.
L. V. Logovskaya; T. B. Bukharova; A. V. Volkov; E. B. Vikhrova; O. V. Makhnach; D. V. Goldshtein. Induction of osteogenic differentiation of multipotent mesenchymal stromal cells from human adipose tissue. Bulletin of Experimental Biology and Medicine 2013, 155, 145 -150.
AMA StyleL. V. Logovskaya, T. B. Bukharova, A. V. Volkov, E. B. Vikhrova, O. V. Makhnach, D. V. Goldshtein. Induction of osteogenic differentiation of multipotent mesenchymal stromal cells from human adipose tissue. Bulletin of Experimental Biology and Medicine. 2013; 155 (1):145-150.
Chicago/Turabian StyleL. V. Logovskaya; T. B. Bukharova; A. V. Volkov; E. B. Vikhrova; O. V. Makhnach; D. V. Goldshtein. 2013. "Induction of osteogenic differentiation of multipotent mesenchymal stromal cells from human adipose tissue." Bulletin of Experimental Biology and Medicine 155, no. 1: 145-150.
I S Alekseeva; Anatoliy Kulakov; D V Gol'dshteĭn; Alexey Volkov. [Bone tissue restoration after tooth removal by means of tissue-engineering construction based on multipotent stromal adipose cells]. Stomatologiya 2012, 91, 1 .
AMA StyleI S Alekseeva, Anatoliy Kulakov, D V Gol'dshteĭn, Alexey Volkov. [Bone tissue restoration after tooth removal by means of tissue-engineering construction based on multipotent stromal adipose cells]. Stomatologiya. 2012; 91 (4):1.
Chicago/Turabian StyleI S Alekseeva; Anatoliy Kulakov; D V Gol'dshteĭn; Alexey Volkov. 2012. "[Bone tissue restoration after tooth removal by means of tissue-engineering construction based on multipotent stromal adipose cells]." Stomatologiya 91, no. 4: 1.
I S Alekseeva; O A Rachinskaia; Alexey Volkov; Anatoliy Kulakov; D V Gol'dshteĭn. [A comparative evaluation of bone tissue formation by tissue scaffold and osteoplastic material «Bio-Oss» transplantation in the maxillary sinus floor]. Stomatologiya 2012, 91, 1 .
AMA StyleI S Alekseeva, O A Rachinskaia, Alexey Volkov, Anatoliy Kulakov, D V Gol'dshteĭn. [A comparative evaluation of bone tissue formation by tissue scaffold and osteoplastic material «Bio-Oss» transplantation in the maxillary sinus floor]. Stomatologiya. 2012; 91 (6):1.
Chicago/Turabian StyleI S Alekseeva; O A Rachinskaia; Alexey Volkov; Anatoliy Kulakov; D V Gol'dshteĭn. 2012. "[A comparative evaluation of bone tissue formation by tissue scaffold and osteoplastic material «Bio-Oss» transplantation in the maxillary sinus floor]." Stomatologiya 91, no. 6: 1.
We developed a new method of creation of tissue engineering constructs for regeneration of the bone tissue based on the principle of free distribution of cells in a fibrin clot within a scaffold. The tissue engineering construct includes multipotent stromal adipose tissue cells committed in osteogenic lineage, platelet-rich plasma, and resorbed material on the basis of xenogeneic bone collagen. The culture of bone progenitor cells was characterized by the main markers of osteoblastic differon. The material meets all requirements for materials intended for tissue engineering. An innovative high-technological tissue engineering product for clinical application is prepared.
T. B. Bukharova; Irina Arutyunyan; S. A. Shustrov; I. S. Alekseeva; I. A. Fedyunina; L. V. Logovskaya; Alexey Volkov; A. A. Rzhaninova; A. S. Grigor’Yan; Anatoliy Kulakov; D. V. Gol’Dshtein. Tissue Engineering Construct on the Basis of Multipotent Stromal Adipose Tissue Cells and Osteomatrix for Regeneration of the Bone Tissue. Bulletin of Experimental Biology and Medicine 2011, 152, 153 -158.
AMA StyleT. B. Bukharova, Irina Arutyunyan, S. A. Shustrov, I. S. Alekseeva, I. A. Fedyunina, L. V. Logovskaya, Alexey Volkov, A. A. Rzhaninova, A. S. Grigor’Yan, Anatoliy Kulakov, D. V. Gol’Dshtein. Tissue Engineering Construct on the Basis of Multipotent Stromal Adipose Tissue Cells and Osteomatrix for Regeneration of the Bone Tissue. Bulletin of Experimental Biology and Medicine. 2011; 152 (1):153-158.
Chicago/Turabian StyleT. B. Bukharova; Irina Arutyunyan; S. A. Shustrov; I. S. Alekseeva; I. A. Fedyunina; L. V. Logovskaya; Alexey Volkov; A. A. Rzhaninova; A. S. Grigor’Yan; Anatoliy Kulakov; D. V. Gol’Dshtein. 2011. "Tissue Engineering Construct on the Basis of Multipotent Stromal Adipose Tissue Cells and Osteomatrix for Regeneration of the Bone Tissue." Bulletin of Experimental Biology and Medicine 152, no. 1: 153-158.
We performed a comparative study of reparative osteogenesis in rabbits with experimental critical defects of the parietal bones after implantation of commercial osteoinductive materials "Biomatrix", "Osteomatrix", "BioOss" in combination with platelet-rich plasma and transplantation of a tissue-engineering construct on the basis of autogenic multipotent stromal cells from the adipose tissue predifferentiated in osteogenic direction. It was found that experimental reparative osteogenesis is insufficiently stimulated by implantation materials and full-thickness trepanation holes were not completely closed. After transplantation of the studied tissue-engineering construct, the defect was filled with full-length bone regenerate (in the center of the regenerate and from the maternal bone) in contrast to control and reference groups, where the bone tissue was formed only on the side of the maternal bone. On day 120 after transplantation of the tissue-engineering construct, the percent of newly-formed bone tissue in the regenerate was 24% (the total percent of bone tissue in the regenerate was 39%), which attested to active incomplete regenerative process in contrast to control and reference groups. Thus, the study demonstrated effective regeneration of the critical defects of the parietal bones in rabbits 120 days after transplantation of the tissue-engineering construct in contrast to commercial osteoplastic materials for directed bone regeneration.
A. V. Volkov; I. S. Alekseeva; A. A. Kulakov; D. V. Gol’Dshtein; S. A. Shustrov; A. I. Shuraev; I. V. Arutyunyan; T. B. Bukharova; A. A. Rzhaninova; G. B. Bol’Shakova; A. S. Grigor’Yan. Regeneration of skull bones in adult rabbits after implantation of commercial osteoinductive materials and transplantation of a tissue-engineering construct. Bulletin of Experimental Biology and Medicine 2010, 149, 505 -510.
AMA StyleA. V. Volkov, I. S. Alekseeva, A. A. Kulakov, D. V. Gol’Dshtein, S. A. Shustrov, A. I. Shuraev, I. V. Arutyunyan, T. B. Bukharova, A. A. Rzhaninova, G. B. Bol’Shakova, A. S. Grigor’Yan. Regeneration of skull bones in adult rabbits after implantation of commercial osteoinductive materials and transplantation of a tissue-engineering construct. Bulletin of Experimental Biology and Medicine. 2010; 149 (4):505-510.
Chicago/Turabian StyleA. V. Volkov; I. S. Alekseeva; A. A. Kulakov; D. V. Gol’Dshtein; S. A. Shustrov; A. I. Shuraev; I. V. Arutyunyan; T. B. Bukharova; A. A. Rzhaninova; G. B. Bol’Shakova; A. S. Grigor’Yan. 2010. "Regeneration of skull bones in adult rabbits after implantation of commercial osteoinductive materials and transplantation of a tissue-engineering construct." Bulletin of Experimental Biology and Medicine 149, no. 4: 505-510.
A method for isolation of homogenous culture of cells expressing CD146 marker from adipose tissue lipoaspirate was developed. The resultant clonogenic cultures retained high proliferative activity, immunophenotype, and morphology after numerous passages. The presence of insulin in the medium served as the selective factor for maintenance of the population phenotype. These cultures effectively differentiate into CD31(+)endothelial cells and can be used in regenerative medicine.
A. A. Rzhaninova; A. V. Kulikov; I. A. Spirova; E. E. Kirienko; A. V. Volkov; D. V. Goldshtein. Preparation and characterization of culture of CD146+ cells from human adipose tissue. Bulletin of Experimental Biology and Medicine 2010, 149, 113 -118.
AMA StyleA. A. Rzhaninova, A. V. Kulikov, I. A. Spirova, E. E. Kirienko, A. V. Volkov, D. V. Goldshtein. Preparation and characterization of culture of CD146+ cells from human adipose tissue. Bulletin of Experimental Biology and Medicine. 2010; 149 (1):113-118.
Chicago/Turabian StyleA. A. Rzhaninova; A. V. Kulikov; I. A. Spirova; E. E. Kirienko; A. V. Volkov; D. V. Goldshtein. 2010. "Preparation and characterization of culture of CD146+ cells from human adipose tissue." Bulletin of Experimental Biology and Medicine 149, no. 1: 113-118.
We studied the biocompatibility of porous polylactide carrier matrices obtained by means of surface selective laser sintering. Carrier matrices had no cytotoxic activity, but maintained adhesion and proliferation of cells. Subcutaneous transplantation of tissue engineering constructions from these carriers and bone marrow-derived multipotent stromal cells did not cause the inflammatory response and pathological changes in rats. The conditions for organotypic regeneration were provided at the site of transplantation (high degree of blood supply and considerable amount of immature precursor cells).
T. B. Bukharova; E. N. Antonov; Vladimir Popov; Timur Fatkhudinov; A. V. Popova; Alexey Volkov; S. A. Bochkova; V. N. Bagratashvili; D. V. Gol’Dshtein. Biocompatibility of Tissue Engineering Constructions from Porous Polylactide Carriers Obtained by the Method of Selective Laser Sintering and Bone Marrow-Derived Multipotent Stromal Cells. Bulletin of Experimental Biology and Medicine 2010, 149, 148 -153.
AMA StyleT. B. Bukharova, E. N. Antonov, Vladimir Popov, Timur Fatkhudinov, A. V. Popova, Alexey Volkov, S. A. Bochkova, V. N. Bagratashvili, D. V. Gol’Dshtein. Biocompatibility of Tissue Engineering Constructions from Porous Polylactide Carriers Obtained by the Method of Selective Laser Sintering and Bone Marrow-Derived Multipotent Stromal Cells. Bulletin of Experimental Biology and Medicine. 2010; 149 (1):148-153.
Chicago/Turabian StyleT. B. Bukharova; E. N. Antonov; Vladimir Popov; Timur Fatkhudinov; A. V. Popova; Alexey Volkov; S. A. Bochkova; V. N. Bagratashvili; D. V. Gol’Dshtein. 2010. "Biocompatibility of Tissue Engineering Constructions from Porous Polylactide Carriers Obtained by the Method of Selective Laser Sintering and Bone Marrow-Derived Multipotent Stromal Cells." Bulletin of Experimental Biology and Medicine 149, no. 1: 148-153.
We studied morphological changes in the paraurethral area of Wistar rats after introduction of tissue engineering constructs on the basis of multipotent mesenchymal stem cells and gelatin sponge. The tissue engineering construct containing autologous culture of the stromal fraction of the adipose tissue was most effective. After introduction of this construct we observed more rapid degradation of the construct matrix and more intensive formation of collagen fibers.
A. V. Makarov; Irina Arutyunyan; G. B.Bol’Shakova; Alexey Volkov; D. V. Gol’Dshtein. Morphological Changes in Paraurethral Area after Introduction of Tissue Engineering Construct on the Basis of Adipose Tissue Stromal Cells. Bulletin of Experimental Biology and Medicine 2009, 148, 719 -724.
AMA StyleA. V. Makarov, Irina Arutyunyan, G. B.Bol’Shakova, Alexey Volkov, D. V. Gol’Dshtein. Morphological Changes in Paraurethral Area after Introduction of Tissue Engineering Construct on the Basis of Adipose Tissue Stromal Cells. Bulletin of Experimental Biology and Medicine. 2009; 148 (4):719-724.
Chicago/Turabian StyleA. V. Makarov; Irina Arutyunyan; G. B.Bol’Shakova; Alexey Volkov; D. V. Gol’Dshtein. 2009. "Morphological Changes in Paraurethral Area after Introduction of Tissue Engineering Construct on the Basis of Adipose Tissue Stromal Cells." Bulletin of Experimental Biology and Medicine 148, no. 4: 719-724.
Study of tumorigenic activity of cultured human chondroblasts intended for autologous transplantations showed that subcutaneous injection of human chondroblast suspension in a dose of 10(7)cells/0.2 ml did not cause the development of tumors in C.B-17 SCID immunodeficient mice over 12 weeks. Subcutaneous formations of compact cartilaginous consistency persisted at the site of chondroblast transplantation in all animals; these formations gradually shrank. Subcutaneous injection of tumor cell suspension (A204 human embryonic sarcoma) to C.B-17 SCID mice under the same conditions caused 100% development of tumors (poorly differentiated sarcomas) by day 14.
A. A. Rzhaninova; N. P. Ermakova; I. B. Merkulova; A. I. Mishin; I. V. Arutjunian; A. V. Volkov; L. M. Mikhailova; A. N. Murashev; D. V. Goldshtein. Study of the safety of transplantation of cultured autologous human chondroblasts. Bulletin of Experimental Biology and Medicine 2009, 148, 126 -131.
AMA StyleA. A. Rzhaninova, N. P. Ermakova, I. B. Merkulova, A. I. Mishin, I. V. Arutjunian, A. V. Volkov, L. M. Mikhailova, A. N. Murashev, D. V. Goldshtein. Study of the safety of transplantation of cultured autologous human chondroblasts. Bulletin of Experimental Biology and Medicine. 2009; 148 (1):126-131.
Chicago/Turabian StyleA. A. Rzhaninova; N. P. Ermakova; I. B. Merkulova; A. I. Mishin; I. V. Arutjunian; A. V. Volkov; L. M. Mikhailova; A. N. Murashev; D. V. Goldshtein. 2009. "Study of the safety of transplantation of cultured autologous human chondroblasts." Bulletin of Experimental Biology and Medicine 148, no. 1: 126-131.
Effect of dexamethasone on differentiation of multipotent stromal cells from human adipose tissue was evaluated. Addition of dexamethasone to growth medium resulted in active adipogenesis. Addition of dexamethasone to the osteogenic medium (containing active vitamin D3 form as the main inductor) led to simultaneous realization of the adipogenic and osteogenic potencies of multipotent stromal cells of the adipose tissue. Hence, the quality of the transplant on the basis of predifferentiated multipotent stromal cells from the adipose tissue for bone tissue repair can be deteriorated by dexamethasone directing some cells to adipogenic development.
Irina Arutyunyan; A. A. Rzhaninova; Alexey Volkov; And D. V. Goldstein. Effect of Dexamethasone on Differentiation of Multipotent Stromal Cells from Human Adipose Tissue. Bulletin of Experimental Biology and Medicine 2009, 147, 503 -508.
AMA StyleIrina Arutyunyan, A. A. Rzhaninova, Alexey Volkov, And D. V. Goldstein. Effect of Dexamethasone on Differentiation of Multipotent Stromal Cells from Human Adipose Tissue. Bulletin of Experimental Biology and Medicine. 2009; 147 (4):503-508.
Chicago/Turabian StyleIrina Arutyunyan; A. A. Rzhaninova; Alexey Volkov; And D. V. Goldstein. 2009. "Effect of Dexamethasone on Differentiation of Multipotent Stromal Cells from Human Adipose Tissue." Bulletin of Experimental Biology and Medicine 147, no. 4: 503-508.
I S Alekseeva; I V Arutiunian; Alexey Volkov; A I Shuraev. [Combined cell transplant based upon autologic multipotent stromal cells of adipose tissue use in patients with distinct deficit of bone tissue in maxillae region]. Stomatologiya 2009, 88, 1 .
AMA StyleI S Alekseeva, I V Arutiunian, Alexey Volkov, A I Shuraev. [Combined cell transplant based upon autologic multipotent stromal cells of adipose tissue use in patients with distinct deficit of bone tissue in maxillae region]. Stomatologiya. 2009; 88 (6):1.
Chicago/Turabian StyleI S Alekseeva; I V Arutiunian; Alexey Volkov; A I Shuraev. 2009. "[Combined cell transplant based upon autologic multipotent stromal cells of adipose tissue use in patients with distinct deficit of bone tissue in maxillae region]." Stomatologiya 88, no. 6: 1.
We present an injection form of tissue-engineering construct on the basis of autogenous human chondroblasts for regeneration of the cartilaginous tissue. A material meeting the requirements for the creation of the injection form of an ITC construct (injectable chondrocyte transplantation) was used as the matrix carrier. The developed construct on the basis of autogenous human chondroblasts and homogenized hemostatic gelatin sponge is now at the stage of complex experimental testing.
I. V. Arutjunjan; A. V. Volkov; T. B. Bukharova; P. V. Zelenkov; N. A. Konovalov; A. A. Rzhaninova; I. N. Shevelev; D. V. Goldshtein. Injection form of tissue-engineering construct on the basis of autogenous chondroblasts for regeneration of the cartilaginous tissue. Bulletin of Experimental Biology and Medicine 2008, 146, 501 -505.
AMA StyleI. V. Arutjunjan, A. V. Volkov, T. B. Bukharova, P. V. Zelenkov, N. A. Konovalov, A. A. Rzhaninova, I. N. Shevelev, D. V. Goldshtein. Injection form of tissue-engineering construct on the basis of autogenous chondroblasts for regeneration of the cartilaginous tissue. Bulletin of Experimental Biology and Medicine. 2008; 146 (4):501-505.
Chicago/Turabian StyleI. V. Arutjunjan; A. V. Volkov; T. B. Bukharova; P. V. Zelenkov; N. A. Konovalov; A. A. Rzhaninova; I. N. Shevelev; D. V. Goldshtein. 2008. "Injection form of tissue-engineering construct on the basis of autogenous chondroblasts for regeneration of the cartilaginous tissue." Bulletin of Experimental Biology and Medicine 146, no. 4: 501-505.
The use of synthetic osteoplastic materials not always provides the required amount of the bone tissue. Transplantation of tissue-engineering constructs containing osteogenic precursor cells can be an alternative high-technology implantation method. Here we present the results of a pilot clinical study demonstrating safety of this method, accelerated healing of the operation wound, formation of young bone tissue after transplantation, and the possibility of mounting implants after 3 months in case of sufficient amount of the bone for primary fixation.
A. A. Kulakov; D. V. Goldshtein; A. S. Grigoryan; A. A. Rzhaninova; I. S. Alekseeva; I. V. Arutyunyan; A. V. Volkov. Clinical Study of the Efficiency of Combined Cell Transplant on the Basis of Multipotent Mesenchymal Stromal Adipose Tissue Cells in Patients with Pronounced Deficit of the Maxillary and Mandibulary Bone Tissue. Bulletin of Experimental Biology and Medicine 2008, 146, 522 -525.
AMA StyleA. A. Kulakov, D. V. Goldshtein, A. S. Grigoryan, A. A. Rzhaninova, I. S. Alekseeva, I. V. Arutyunyan, A. V. Volkov. Clinical Study of the Efficiency of Combined Cell Transplant on the Basis of Multipotent Mesenchymal Stromal Adipose Tissue Cells in Patients with Pronounced Deficit of the Maxillary and Mandibulary Bone Tissue. Bulletin of Experimental Biology and Medicine. 2008; 146 (4):522-525.
Chicago/Turabian StyleA. A. Kulakov; D. V. Goldshtein; A. S. Grigoryan; A. A. Rzhaninova; I. S. Alekseeva; I. V. Arutyunyan; A. V. Volkov. 2008. "Clinical Study of the Efficiency of Combined Cell Transplant on the Basis of Multipotent Mesenchymal Stromal Adipose Tissue Cells in Patients with Pronounced Deficit of the Maxillary and Mandibulary Bone Tissue." Bulletin of Experimental Biology and Medicine 146, no. 4: 522-525.