Details var a2a_config = a2a_config || {}; a2a_config.onclick = 1; a2a_config.locale = "pl"; a2a_config.color_main = "D7E5ED"; a2a_config.color_border = "9D9D9D"; a2a_config.color_link_text = "ffffff"; a2a_config.color_link_text_hover = "ffffff"; a2a_config.color_bg = "d9d9d9"; a2a_config.icon_color = "#4e4e4e"; a2a_config.exclude_services = ["dihitt", "delicious", "whatsapp", "dzone", "sms", "stumpedia", "viber", "yahoo_messenger", "google_plus"]; Details PDF BIBTEX RIS Title Changes in lipid metabolism during last month of pregnancy and first two months of lactation in primiparous cows – analysis of apolipoprotein expression pattern and changes in concentration of total cholesterol, HDL, LDL, triglycerides Journal title Polish Journal of Veterinary Sciences Yearbook 2015 Issue No 2 Authors Kurpińska, A.K. ; Jarosz, A. ; Ożgo, M. ; Skrzypczak, W.F. Divisions of PAS Nauki Biologiczne i Rolnicze Publisher Polish Academy of Sciences Committee of Veterinary Sciences ; University of Warmia and Mazury in Olsztyn Date 2015[2015.01.01 AD - 2015.12.31 AD] Identifier DOI: 10.1515/pjvs-2015-0038 ; ISSN 1505-1773 Source Polish Journal of Veterinary Sciences; 2015; No 2 References Cairoli (2006), Serum protein pattern during cow pregnancy : Acute - phase proteins increase in the peripartum period, Electrophoresis, 27, 1617, doi.org/10.1002/elps.200500742 ; Burkey (1992), Hepatic apolipoprotein J is secreted as a lipoprotein, J Lipid Res, 1517. ; Blum (1982), Dynamics of apolipoprotein E metabolism in humans, J Lipid Res, 1308. ; Uchida (1993), Appearance of haptoglobin in serum from cows at parturition, J Vet Med Sci, 893, doi.org/10.1292/jvms.55.893 ; Marcos (1990), The effect of pregnancy and lactation on serum lipid and apolipoprotein B and A - I levels in dairy cows, J Anim Physiol Anim Nutr, 133, doi.org/10.1111/j.1439-0396.1990.tb00214.x ; 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The present study was undertaken to determine blood plasma protein and lipid profile changes in healthy Polish Holstein-Fresian calves of Black-and-White variety. Blood was drawn immediately after birth, before first colostrum intake and at the 3rd, 6^th, 12^th, 24^th, 36^th, 48^thand 72^ndhour of life. Subsequent four blood samples were collected at 24 hour intervals until the 7th day of life. Plasma proteins within the isoelectric point ranging from 3.0 to 10.0 were separated using high resolution two-dimensional electrophoresis. Among the 74 protein spots detected and analyzed, 16 were significantly altered during the first week of life. Differentially expressed spots were excised from the gels and subjected to peptide mass fingerprinting using MALDI-TOF MS. In total, 12 spots were successfully identified, which correspond to three proteins, namely: apolipoprotein A-I, apolipoprotein A-IV and fibrinogen gamma-B chain. A gradual increase in plasma triglyceride, total cholesterol, HDL and LDL cholesterol values was shown during the first seven days of calves life. The lowest concentration of these indicators were observed at birth and was followed by a rapid increase during the first week of postnatal life. These changes appear to be related to the transition in energy sources, from a maternal nutrient supply comprising mainly carbohydrates and amino acids to a diet which was rich in fat - colostrum and milk. This was reflected by the intense up-regulation of plasma proteins related with lipid transport and lipoprotein metabolism during the first week of life.

The aim of this study was to determine the influence of age and ingested food (colostrum and mature milk) on the concentrations of selected blood biochemical components connected with nitrogen and mineral metabolism in dairy calves during their first week of life. The experiment was carried out on 13 Polish Black and White breed dairy calves. The animals were fed colostrum within the first 3 days of postnatal life and thereafter the mature milk of their dams until the end of the experiment (7 days). The obtained results showed that intensive catabolic and anabolic changes in nitrogen occur in the first week of life. These changes were particularly intense during the first 24-48 hours of life and may reflect dynamic tissue remodelling. The results of this experiment also show that healthy calves efficiently regulate water and electrolyte homeostasis.

The structural proteins of renal tubular epithelial cells may become a target for the toxic metabolites of immunosuppressants. These metabolites can modify the properties of the proteins, thereby affecting cell function, which is a possible explanation for the mechanism of immunosuppressive agents' toxicity. In our study, we evaluated the effect of two immunosuppressive strategies on protein expression in the kidneys of Wistar rats. Fragments of the rat kidneys were homogenized after cooling in liquid nitrogen and then dissolved in lysis buffer. The protein concentration in the samples was determined using a protein assay kit, and the proteins were separated by two-dimensional electrophoresis. The obtained gels were then stained with Coomassie Brilliant Blue, and their images were analyzed to evaluate differences in protein expression. Identification of selected proteins was then performed using mass spectrometry. We found that the immunosuppressive drugs used in popular regimens induce a series of changes in protein expression in target organs. The expression of proteins involved in drug, glucose, amino acid, and lipid metabolism was pronounced. However, to a lesser extent, we also observed changes in nuclear, structural, and transport proteins' synthesis. Very slight differences were observed between the group receiving cyclosporine, mycophenolate mofetil, and glucocorticoids (CMG) and the control group. In contrast, compared to the control group, animals receiving tacrolimus, mycophenolate mofetil, and glucocorticoids (TMG) exhibited higher expression of proteins responsible for renal drug metabolism and lower expression levels of cytoplasmic actin and the major urinary protein. In the TMG group, we observed higher expression of proteins responsible for drug metabolism and a decrease in the expression of respiratory chain enzymes (thioredoxin-2) and markers of distal renal tubular damage (heart fatty acid-binding protein) compared to expression in the CMG group. The consequences of the reported changes in protein expression require further study.