To explore the interaction between the residual antibiotic in animal manure and biological treatment, the effect of enrofloxacin (ENR) on the anaerobic digestion of chicken manure, and biodegradation rate of ENR was studied under the condition of actual residual ENR content of 0, 8, 16 and 32 mg/kg·TS. The results showed that the addition of ENR increased the total biogas production, especially 8 mg/kg·TS promoted the anaerobic reaction obviously, and the corresponding cumulative biogas production was increased by 15.33%. However, in the presence of 32 mg/kg·TS, the biogas production rate was reduced and the peak period of biogas production was delayed. The results of enzyme activities determination and 16S rRNA sequencing showed that ENR had different effects on archaea and bacteria. The residual ENR could promote hydrolysis reactions in the anaerobic system, but could inhibit acetoclastic methanogens, and the relative abundance of Methanosaeta declined by 7.22‒12.41%. The first-order kinetic model showed that the half-life period of ENR in the anaerobic digestion system was 9.16‒10.83 days, and the biodegradation rate exceeded 80% after the treatment. This study can bring important information for the management of animal manure in the future.

The anaerobic fermentation of crop straw and animal wastes is increasingly used for the biogas and green energy generation, as well as reduction of the environmental pollution. The anaerobic cofermentation of corn stalks inoculated by cow dung was found to achieve higher biogas production and cellulose biodegradation. In this study, the effect of mixing corn stalks with cow dung at five different fermentation stages (0, 7, 15, 23, and 31 days of the total fermentation cycle of 60 days) on the further cofermentation process was explored, in order to optimize the corn straw utilization rate and biogas production capacity. In addition, the straw microstructure evolution was investigated by the SEM and XRD methods to identify the optimal conditions for the straw biodegradation process enhancement. The five test groups exhibited nearly identical total biogas productivity values but strongly differed by daily biogas yields (the maximal biogas generation rate being 524.3 ml/d). Based on the degradation characteristics of total solids (TS), volatile solids (VS), and lignocellulose, groups #1 and #3 (0 and 15 days) had the most significant degradation rates of VS (43.73%) and TS (42.07%), respectively, while the largest degradation rates of cellulose (62.70%) and hemicellulose (50.49%) were observed in group #4 (23 days) and group #1 (0 days), respectively. The SEM analysis revealed strong microstructural changes in corn stalks after fermentation manifested by multiple cracks and striations, while the XRD results proved the decrease in peak intensity of cellulose 002 crystal surface and the reduced crystallinity after cofermentation. The results of this study are assumed to be quite instrumental to the further optimization of the corn stalk anaerobic digestion by inoculation with digested manure for lignocellulose degradation enhancement and biogas productivity improvement.

This article proposes a methane production approach through sequenced anaerobic digestion of kitchen waste, determines the hydrolysis constants and reaction orders at both low total solid (TS) concentrations and high TS concentrations using the initial rate method, and examines the population growth model and first-order hydrolysis model. The findings indicate that the first-order hydrolysis model better reflects the kinetic process of gas production. During the experiment, all the influential factors of anaerobic fermentation retained their optimal values. The hydrolysis constants and reaction orders at low TS concentrations are then employed to demonstrate that the first-order gas production model can describe the kinetics of the gas production process. At low TS concentrations, the hydrolysis constants and reaction orders demonstrated opposite trends, with both stabilizing after 24 days at 0.99 and 1.1252, respectively. At high TS concentrations, the hydrolysis constants and the reaction orders stabilized at 0.98 (after 18 days) and 0.3507 (after 14 days), respectively. Given sufficient reaction time, the hydrolysis involved in anaerobic fermentation of kitchen waste can be regarded as a first-order reaction in terms of reaction kinetics. This study serves as a good reference for future studies regarding the kinetics of anaerobic digestion of kitchen waste.

Purpose: To explore the methane production potential and microbial community changes of combined anaerobic digestion of chicken manure and corn straw. Increase methane production, reduce the environmental pollution caused by the burning of livestock manure and straw, and provide some theoretical references for the construction and operation of actual biogas projects. Methods: Different proportions (3%, 5%, 10%) of corn straw were added to the anaerobic digestion systems of chicken manure in order to improve the C/N ratio and to evaluate the feasibility and potential synergistic effect on the co-digestion. The key point was to use 16S rDNA sequencing to analyze the relationship between the microbial diversity and the hydrolase activity during the anaerobic digestion. Result: The results showed that the volumetric gas production of methane in the 3% straw addition group was 227.66 ml/gVS, which was 18% higher than the cumulative methane production in the pure chicken manure experimental group. However, with the increase of straw concentration, methane production and the utilization rate of the raw materials continued to decrease. The change in activity of each hydrolase was in agreement with changes in hydrolytic acidifying bacteria, and the activity of the main hydrolase also increased with the addition of straw; the correlation coefficient was 0.9943. Sequencing results showed that the dominant strains of methanogenic archaea were Methanosarcina, Methanosaeta, Methanobacterium, and Methanospirillum. Mainly for hydrogen-eating, acetic acid-eating methanogens, its role is to use H₂, methanol and acetic acid, and other substances to metabolize methane, and convert it into CH₄ and CO₂. Conclusion: The addition of a small amount of straw enhanced the production capacity of hydrogen-nutritive methane to some extent, and the species richness and evenness were also improved, reducing the pollution caused by livestock manure to the environment while controlling the pollution caused by straw burning. Graphical