Ascertaining when and where genes are expressed is of crucial importance to understanding or predicting the physiological role of genes and proteins and how they interact to form the complex networks that underlie organ development and function. It is, therefore, crucial to determine on a genome-wide level, the spatio-temporal gene expression profiles at cellular resolution. This information is provided by colorimetric RNA in situ hybridization that can elucidate expression of genes in their native context and does so at cellular resolution. We generated what is to our knowledge the first genome-wide transcriptome atlas by RNA in situ hybridization of an entire mammalian organism, the developing mouse at embryonic day 14.5. This digital transcriptome atlas, the Eurexpress atlas (http://www.eurexpress.org), consists of a searchable database of annotated images that can be interactively viewed. We generated anatomy-based expression profiles for over 18,000 coding genes and over 400 microRNAs. We identified 1,002 tissue-specific genes that are a source of novel tissue-specific markers for 37 different anatomical structures. The quality and the resolution of the data revealed novel molecular domains for several developing structures, such as the telencephalon, a novel organization for the hypothalamus, and insight on the Wnt network involved in renal epithelial differentiation during kidney development. The digital transcriptome atlas is a powerful resource to determine co-expression of genes, to identify cell populations and lineages, and to identify functional associations between genes relevant to development and disease. In situ hybridization (ISH) can be used to visualize gene expression in cells and tissues in their native context. High-throughput ISH using nonradioactive RNA probes allowed the Eurexpress consortium to generate a comprehensive, interactive, and freely accessible digital gene expression atlas, the Eurexpress transcriptome atlas (http://www.eurexpress.org), of the E14.5 mouse embryo. Expression data for over 15,000 genes were annotated for hundreds of anatomical structures, thus allowing us to systematically identify tissue-specific and tissue-overlapping gene networks. We illustrate the value of the Eurexpress atlas by finding novel regional subdivisions in the developing brain. We also use the transcriptome atlas to allocate specific components of the complex Wnt signaling pathway to kidney development, and we identify regionally expressed genes in liver that may be markers of hematopoietic stem cell differentiation.

Recent advancements in Large Language Models (LLMs) suggest imminent commercial applications of such AI systems where they will serve as gateways to interact with technology and the accumulated body of human knowledge. The possibility of political biases embedded in these models raises concerns about their potential misusage. In this work, we report the results of administering 15 different political orientation tests (14 in English, 1 in Spanish) to a state-of-the-art Large Language Model, the popular ChatGPT from OpenAI. The results are consistent across tests; 14 of the 15 instruments diagnose ChatGPT answers to their questions as manifesting a preference for left-leaning viewpoints. When asked explicitly about its political preferences, ChatGPT often claims to hold no political opinions and to just strive to provide factual and neutral information. It is desirable that public facing artificial intelligence systems provide accurate and factual information about empirically verifiable issues, but such systems should strive for political neutrality on largely normative questions for which there is no straightforward way to empirically validate a viewpoint. Thus, ethical AI systems should present users with balanced arguments on the issue at hand and avoid claiming neutrality while displaying clear signs of political bias in their content.

I report here a comprehensive analysis about the political preferences embedded in Large Language Models (LLMs). Namely, I administer 11 political orientation tests, designed to identify the political preferences of the test taker, to 24 state-of-the-art conversational LLMs, both closed and open source. When probed with questions/statements with political connotations, most conversational LLMs tend to generate responses that are diagnosed by most political test instruments as manifesting preferences for left-of-center viewpoints. This does not appear to be the case for five additional base (i.e. foundation) models upon which LLMs optimized for conversation with humans are built. However, the weak performance of the base models at coherently answering the tests’ questions makes this subset of results inconclusive. Finally, I demonstrate that LLMs can be steered towards specific locations in the political spectrum through Supervised Fine-Tuning (SFT) with only modest amounts of politically aligned data, suggesting SFT’s potential to embed political orientation in LLMs. With LLMs beginning to partially displace traditional information sources like search engines and Wikipedia, the societal implications of political biases embedded in LLMs are substantial.

This work describes a chronological (2000–2019) analysis of sentiment and emotion in 23 million headlines from 47 news media outlets popular in the United States. We use Transformer language models fine-tuned for detection of sentiment (positive, negative) and Ekman’s six basic emotions (anger, disgust, fear, joy, sadness, surprise) plus neutral to automatically label the headlines. Results show an increase of sentiment negativity in headlines across written news media since the year 2000. Headlines from right-leaning news media have been, on average, consistently more negative than headlines from left-leaning outlets over the entire studied time period. The chronological analysis of headlines emotionality shows a growing proportion of headlines denoting anger, fear, disgust and sadness and a decrease in the prevalence of emotionally neutral headlines across the studied outlets over the 2000–2019 interval. The prevalence of headlines denoting anger appears to be higher, on average, in right-leaning news outlets than in left-leaning news media.

Concerns about gender bias in word embedding models have captured substantial attention in the algorithmic bias research literature. Other bias types however have received lesser amounts of scrutiny. This work describes a large-scale analysis of sentiment associations in popular word embedding models along the lines of gender and ethnicity but also along the less frequently studied dimensions of socioeconomic status, age, physical appearance, sexual orientation, religious sentiment and political leanings. Consistent with previous scholarly literature, this work has found systemic bias against given names popular among African-Americans in most embedding models examined. Gender bias in embedding models however appears to be multifaceted and often reversed in polarity to what has been regularly reported. Interestingly, using the common operationalization of the term bias in the fairness literature, novel types of so far unreported bias types in word embedding models have also been identified. Specifically, the popular embedding models analyzed here display negative biases against middle and working-class socioeconomic status, male children, senior citizens, plain physical appearance and intellectual phenomena such as Islamic religious faith, non-religiosity and conservative political orientation. Reasons for the paradoxical underreporting of these bias types in the relevant literature are probably manifold but widely held blind spots when searching for algorithmic bias and a lack of widespread technical jargon to unambiguously describe a variety of algorithmic associations could conceivably be playing a role. The causal origins for the multiplicity of loaded associations attached to distinct demographic groups within embedding models are often unclear but the heterogeneity of said associations and their potential multifactorial roots raises doubts about the validity of grouping them all under the umbrella term bias. Richer and more fine-grained terminology as well as a more comprehensive exploration of the bias landscape could help the fairness epistemic community to characterize and neutralize algorithmic discrimination more efficiently.

The emergence of small handheld devices such as tablets and smartphones, often with touch sensitive surfaces as their only input modality, has spurred a growing interest in the subject of gestures for human–computer interaction (HCI). It has been proven before that eye movements can be consciously controlled by humans to the extent of performing sequences of predefined movement patterns, or “gaze gestures” that can be used for HCI purposes in desktop computers. Gaze gestures can be tracked noninvasively using a video-based eye-tracking system. We propose here that gaze gestures can also be an effective input paradigm to interact with handheld electronic devices. We show through a pilot user study how gaze gestures can be used to interact with a smartphone, how they are easily assimilated by potential users, and how the Needleman-Wunsch algorithm can effectively discriminate intentional gaze gestures from otherwise typical gaze activity performed during standard interaction with a small smartphone screen. Hence, reliable gaze–smartphone interaction is possible with accuracy rates, depending on the modality of gaze gestures being used (with or without dwell), higher than 80 to 90%, negligible false positive rates, and completion speeds lower than 1 to 1.5 s per gesture. These encouraging results and the low-cost eye-tracking equipment used suggest the possibilities of this new HCI modality for the field of interaction with small-screen handheld devices.