Pathogenic bacteria and antibiotic resistance genes (ARGs) in bioaerosols are major threats to human health. In this study, the microbial community structure and ARG distribution characteristics of airborne bacteria in total suspended particulates (TSP) and PM2.5 were investigated under different air quality levels in Xinxiang, Central China. The results revealed that with the deterioration of air quality, the concentrations of airborne bacteria in both TSP and PM2.5 decreased; however, the relative amounts of pathogenic bacteria increased. The predominant genera in pathogenic bacteria of Bacillus, Sphingomonas, Corynebacterium, Rhodococcus, and Staphylococcus were identified in both TSP and PM2.5. Although the airborne bacteria concentrations and absolute abundances of ARGs in TSP were higher than those in PM2.5 under identical air quality conditions, the bacterial community structure and relative amounts of pathogenic bacteria were similar. In addition, the relationship between environmental factors of ions, metal elements, and meteorological parameters and the community structures of airborne bacteria and pathogenic bacteria were also analyzed. The effects of soluble ions and metal elements on several dominant genera of total bacteria and pathogenic bacteria differed, probably due to the strong tolerance of pathogenic bacteria to harsh atmospheric environments Different subtypes of ARGs showed various distribution characteristics with variations in air quality. The deterioration of air quality can inhibit the dissemination of ARGs, as the minimum values of all ARGs and class 1 integrase intI1 were observed under Severely Polluted conditions. This study provides a comprehensive understanding of the effect of air pollution levels on the airborne bacteria community composition and ARG distribution.
Municipal wastewater treatment plants (MWWTPs) are considered significant artificial sources of greenhouse gas (GHG) emissions, in the forms of methane (CH4) and nitrous oxide (N2O), during their normal operations. In this study, we used an emission factor method to determine the spatial and temporal distribution characteristics of GHG emissions from MWWTPs in China during the period 2005–2014; influencing factors and uncertainties were also analyzed. Our results show that total GHG emissions from Chinese MWWTPs increased from 326.54 to 1294.03 Gg CO2‐eq between 2005 and 2014, and that regional distribution was extremely variable. During this decade, the proportion of CH4 in the total GHG emissions decreased from 74.1 to 59.4% while that of N2O increased from 25.9 to 40.6%. The observed increase in N2O was probably due to the enhancement of wastewater discharge standards for nitrogen discharge, resulting in lower waterborne but higher atmospheric levels of nitrogen oxides. Our comparison of GHG emissions from wastewater discharging directly to the aquatic environment with that treated at MWWTPs in 2014 indicate that the latter disposal method resulted in an 18‐fold drop in GHG emissions. Regional economic development level and wastewater treatment capacity were the factors most closely related to GHG emissions from MWWTPs; the per‐capita protein supply was closely related to N2O emissions.
1 February 2016 in Biochemical Engineering Journal