Several studies in humans indicate that low birth weight predisposes individuals to obesity in later life. Despite the constant increase in prevalence of obesity in the canine population and the major health consequences of this affection, few investigations have been carried out on the association between birth weight and the development of overweight in dogs. The purpose of the current study was to examine the association between birth weight and some other neonatal characteristics and overweight at adulthood in a population of purebred Labrador dogs. Information was collected about the sex, age, neuter status, birth weight, and growth rates (between 0–2 days and 2–15 days of age) in 93 Labrador dogs raised under similar environmental conditions until two months old. The body condition scores (BCS, scale of 1–9) of these dogs at adulthood were recorded, with BCS equal to or greater than 6 classified as overweight. Dogs were split into two groups based on the median birth weight in the population: lower than the median (LTM) and higher than the median (HTM). A logistic regression model was applied to analyse associations between the general characteristics of the dogs (sex, age, neuter status), early life parameters (birth weight, growth rates) and overweight at adulthood. Birth weight was the only early-life parameter found to be associated with overweight (p value = 0.032) with a prevalence of overweight of 70% among the dogs with LTM birth weight vs. 47% in dogs born with HTM birth weight. Overweight was also associated with age and neuter status (p value = 0.029 and 0.005 respectively). Our results suggest that, as in humans, dogs with the lowest birth weights are more likely to become overweight at adulthood. More studies are needed to further examine this relationship and to explore the underlying mechanisms. A subsequent objective could be to identify preventive strategies such as an adapted early nutrition programme for at-risk individuals.

It is generally accepted that microbes play a critical role in maintaining gut barrier function, making them ideal to target in order to mitigate the effects of intestinal diseases such as inflammatory bowel disease with specialist supplementations such as probiotic or postbiotic preparations. In this study, specific strains of Lactobacillus helvictus both live and inactivated and Lactobacillus plantarum inactivated were fed to zebrafish at an inclusion level of 6 × 10⁶ cells/g in order to assess the effects on gut barrier function and protection. Taken together, our results indicate that dietary administration of pro- or postbiotics strengthens the gut barrier function and innate immunity of healthy zebrafish in a strain-specific and process-dependent way. With some differences in the response intensity, the three treatments led to increased intestinal villi length and proportion of IELs, reinforcement of the GC population and up-regulated expression of biomarkers of AMP production and tight junction zona-occludin 2a (zo-2a). In addition, LPPost had an impact on the adaptive immune response, and we hypothesized that it conferred the potential to drive Th17/ILC3 immunity, as suggested by its effect on the gene expression of il22, of different AMPs, and the expression of zo2a. Moreover, LPPost showed the potential to drive Th1/ILC1-like immunity, with a higher percentage of CD8⁺ cells and higher ifnγ gene expression. In summary, the use of inactivated Lactobacilli species in this study represented a promising strategy for improving barrier function and regulating the immune fate of the intestinal mucosa in a strain-specific way.

Low birth weight (LBW) has been identified as a major risk factor for neonatal mortality in many species. The aim of this survey was to determine the profiles of canine and feline breeders concerning their perceptions of, and management practices relating to, LBW individuals. An anonymous online survey was addressed to French cat and dog breeders in September 2019 via social networks. Multiple correspondence analysis and hierarchical clustering were used to explore breeders’ profiles. Three clusters were identified among the 649 breeders included in this analysis. Cluster 1 (49%) included dog and cat breeders who weighed newborns (and thus identified LBW) and controlled nursing by the dam (controlled suckling) but did not warm them up. Cluster 2 breeders (21%) of both species did not weigh puppies or kittens to identify LBW or to monitor the evolution of their weight afterwards. Cluster 3 (30%) including mostly cat breeders who weighed neonates routinely as in Cluster 1, but they practiced artificial feeding rather than controlled suckling. This survey provides a basis for better understanding of perceptions and practices regarding LBW puppies and kittens. It will be useful to provide guidelines for neonatal management to increase their chances of survival.

Time around parturition is a stressful period for both bitches and their puppies. The use of probiotics has been proposed, e.g., in pigs, to improve health status of sows, their reproductive performances and in turn, the health and performance of their progeny. The objective of the present study was to evaluate the impact, on both dams and puppies, of a supplementation of bitches with the live yeast Saccharomyces cerevisiae var. boulardii CNCM I-1079 (SB-1079) during the second part of the gestation and the lactation period. A total of 36 bitches of medium and large-sized breeds were enrolled. They were divided into two groups, one of which received 1.3 × 10⁹ colony forming units of live yeast per day. At dam’s level, SB-1079 yeast shaped a different microbiota structure between the two groups just after whelping, impacted alpha diversity and some plasma metabolites related to energy metabolism. Regarding reproductive performances, SB-1079 improved gross energy of the colostrum (1.4 vs. 1.2 kcal of ME/g) as well as the concentration of protein in milk at Day 7 after parturition (10.4 vs. 7.6%). SB-1079 also reduced the odds of having low birth weight in the litter. At puppy’s level, a modulation of immunometabolic phenotype is suggested by the observation of increased growth rates during the early pediatric period (i.e., between 21 and 56 days of life, 225 vs. 190%) and a decrease of the IL-8:IL-10 ratio after vaccination against rabies (4.2 vs. 16.9). Our findings suggest that SB-1079 supplementation during gestation and lactation has the potential to enhance health of bitches and in turn health of puppies through maternal programming.

Birth weight is one of the earliest health parameters with short (neonatal period) and long term (adulthood) implications for an individual. The present work was conducted on the domestic cat, with the objective of determining factors affecting kitten birth weight. Data voluntarily shared by 139 French breeders allowed building a large dataset of purebred kittens (n = 3,547) from 15 breeds. A linear mixed model with queen and cattery as random terms was used to investigate variation factors of kitten birth weight such as breed, litter size, season of birth, age of the queen, presence of stillborn in the litter, sex of the kitten. The most important factor was breed which explained 25% of the variation in birth weight observed in the study population. The five other parameters were also significant but explained only a small additional part of the variance (less than 3% each). Analyses showed that kitten birth weight increased with the age of the queen, was higher in males than females and in litters without stillbirth than in litters with at least one stillborn. In addition, lower birth weights were recorded in summer and autumn compared to other seasons, and birth weight values decreased as the number of kittens in the litter increased. In order to group feline breeds according to their average birth weights and litter sizes, a K-means algorithm was used to identify three clusters among the 15 breeds represented (Group 1: small litter sizes/low birth weights; Group 2: large litter sizes/intermediate birth weights; Group 3: large litter sizes/high birth weights). This study, based on a large dataset established at the national scale, provides reference values of feline birth weights for breeders and veterinarians. The next step could be to explore the relationship between birth weight and neonatal mortality to help identify neonates requiring specific care.

The aim of this study was to evaluate the diagnostic accuracy of 5 indirect methods that may be used in veterinary practices to assess the success of maternal immunoglobulins transfer in neonatal calves. Blood samples (n = 245) were collected from 2- to 6-d-old calves. Serum immunoglobulin concentrations were determined by radial immunodiffusion. Serum total protein and albumin concentrations and gamma-glutamyl transferase activity were determined by veterinary practitioners using their own biochemistry analyzer. Globulin concentration was calculated. Veterinary practitioners were also asked to assess serum total protein using a digital Brix refractometer (%Brix) and an optical refractometer (g/L). Test sensitivity (Se) and specificity (Sp) were calculated using radial immunodiffusion as the reference standard, with failure of transfer of passive immunity defined as IgG <10.0 g/L. Receiver operating characteristics curves were created and optimal cutoff values for each of the 5 indirect measurement methods were selected based on the maximization of the Youden's J statistic. In this study, Globulin concentration yielded the highest accuracy to identify failure of transfer of passive immunity (Se = 89.4%; Sp = 89.3% at 34 g/L). For %Brix, the optimal combination of Se and Sp was at 8.4% Brix (Se = 86.5% and Sp = 83.8%). The use of an optical refractometer was associated with the lowest diagnostic accuracy (Se = 69.7% and Sp = 81.6% at 52 g/L). For gamma-glutamyl transferase activity, the optimal cut-points were different for 2- to 3-d-old calves (Se = 87.5% and Sp = 87.8% at 393 IU/L) or 4- to 6-d-old calves (Se = 90.0% and Sp = 86.4% at 254 IU/L). Because it does not require any specialized laboratory equipment, digital Brix refractometer can be a valuable and inexpensive tool for on-farm use, to help producers and veterinary practitioners in their calf health management programs.