Purpose: Traditional undergraduate radiographer training mixes academic lectures and clinical practice. Our goal is to bridge the current disconnection between theory and practice in a safe environment, avoiding the risk of radiation for both practitioners and patients. To this end, this research proposes a new software to teach diagnostic radiography using real-time interactive X-ray simulation and patient positioning. Methods: The proposed medical simulator is composed of three main modules. A fast and accurate character animation technique is in charge of simulating the patient positioning phase and adapts their internal anatomy accordingly. gVirtualXRay is an open-source X-ray simulation library and generates the corresponding radiographs in real time. Finally, the courseware allows going through all the diagnostic radiology steps from the patient positioning and the machine configuration to the final image enhancing. Results: A face and content validation study has been conducted; 18 radiology professionals were recruited to evaluate our software using a questionnaire. The results show that our tool is realistic in many ways (72% of the participants agreed that the simulations are visually realistic), useful (67%) and suitable (78%) for teaching X-ray radiography. Conclusions: The proposed tool allows simulating the most relevant steps of the projectional radiography procedure. The virtual patient posing system and X-ray simulation module execute at interactive rates. These features enable the lectures to show their students the results of good and bad practices in a classroom environment, avoiding radiation risk.

The morphological analysis of dendritic spines is an important challenge for the neuroscientific community. Most state-of-the-art techniques rely on user-supervised algorithms to segment the spine surface, especially those designed for light microscopy images. Therefore, processing large dendritic branches is costly and time-consuming. Although deep learning (DL) models have become one of the most commonly used tools in image segmentation, they have not yet been successfully applied to this problem. In this article, we study the feasibility of using DL models to automatize spine segmentation from confocal microscopy images. Supervised learning is the most frequently used method for training DL models. This approach requires large data sets of high-quality segmented images (ground truth). As mentioned above, the segmentation of microscopy images is time-consuming and, therefore, in most cases, neuroanatomists only reconstruct relevant branches of the stack. Additionally, some parts of the dendritic shaft and spines are not segmented due to dyeing problems. In the context of this research, we tested the most successful architectures in the DL biomedical segmentation field. To build the ground truth, we used a large and high-quality data set, according to standards in the field. Nevertheless, this data set is not sufficient to train convolutional neural networks for accurate reconstructions. Therefore, we implemented an automatic preprocessing step and several training strategies to deal with the problems mentioned above. As shown by our results, our system produces a high-quality segmentation in most cases. Finally, we integrated several postprocessing user-supervised algorithms in a graphical user interface application to correct any possible artifacts.

The bottleneck for progress in many research areas within neuroscience has shifted from the data acquisition to the data analysis stages. In the present article, we propose a method named InTool Explorer that we have developed to perform interactive exploratory data analysis, focusing on neuroanatomy as an example of its utility. This tool is freely-available software that has been designed to facilitate the study of complex neuroscience data. InTool Explorer requires no more than an internet connection and a web browser. The main contribution of this tool is to provide a user-designed canvas for data visualization and interaction, to perform specific exploratory tasks according to the user needs. Moreover, InTool Explorer permits visualization of the datasets in a very dynamic and versatile way using a linked-card approach. For this purpose, the tool allows the user to select among different predefined card types. Each card type offers an abstract data representation, a filtering tool or a set of statistical analysis methods. Additionally, InTool Explorer makes it possible linking raw images to the data. These images can be used by InTool Explorer to define new customized filtering cards. Another significant contribution of this tool is that it allows fast visualization of the data, error finding, and re-evaluation to establish new hypotheses or new lines of research. Thus, regarding its practical application in the laboratory, InTool Explorer provides a new opportunity to study and analyze neuroscience data prior to any statistical analysis being carried out.

The analysis and exploration of complex data sets are common problems in many areas, including scientific and business domains. This need has led to substantial development of the data visualization field. However, the diversity of problems to which visual data analysis is applied hinders the implementation of a universal solution that meets the current and future needs of all disciplines. In this paper, we present VMetaFlow, a graphical meta-framework to design interactive and coordinated views applications for data visualization. Our meta-framework is based on data flow diagrams since they have proved their value in simplifying the design of data visualizations. VMetaFlow operates as an abstraction layer that encapsulates and interconnects visualization frameworks in a web-based environment, providing them with interoperability mechanisms. The only requirement is that the visualization framework must be accessible through a JavaScript API. We propose a novel data flow model that allows users to define both interactions between multiple data views and how the data flows between visualization and data processing modules. In contrast with previous data-flow-based frameworks for visualization, we separate the view interactions from data items, broadening the expressiveness of our model and supporting the most common types of multi-view interactions. Our meta-framework allows visualization and data analysis experts to focus their efforts on creating data representations and transformations for their applications, whereas nonexperts can reuse previously developed components to design their applications through a user-friendly interface. We validate our approach through a critical inspection with visualization experts and two case studies. We have carefully selected these case studies to illustrate its capabilities. Finally, we compare our approach with the subset flow model designed for multiple coordinated views.

Technological advances enable the capture and management of complex data sets that need to be correctly understood. Visualisation techniques can help in complex data analysis and exploration, but sometimes the visual channel is not enough, or it is not always available. Some authors propose using the haptic channel to reinforce or substitute the visual sense, but the limited human haptic short-term memory still poses a challenge. We present the haptic tuning fork, a reference signal displayed before the haptic information for increasing the discriminability of haptic icons. With this reference, the user does not depend only on short-term memory. We have decided to evaluate the usefulness of the haptic tuning fork in impedance kinesthetic devices as these are the most common. Furthermore, since the renderable signal ranges are device-dependent, we introduce a methodology to select a discriminable set of signals called the haptic scale. Both the haptic tuning fork and the haptic scale proved their usefulness in the performed experiments regarding haptic stimuli varying in frequency.