The essential oil (EO) was extracted from aerial parts with insecticidal and fungicidal activity. Herein, the hydro-distilled essential oils of Seseli mairei H. Wolff roots were determined by GC-MS. A total of 37 components were identified, (E)-beta-caryophyllene (10.49%), β-geranylgeranyl (6.64%), (E)-2-decenal (6.17%) and germacrene-D (4.28%). The essential oil of Seseli mairei H. Wolff had nematicidal toxicity against Bursaphelenchus xylophilus with a LC₅₀ value of 53.45 μg/mL. The subsequent bioassay-guided investigation led to the isolation of three active constituents: falcarinol, (E)-2-decenal, and octanoic acid. The falcarinol demonstrated the strongest toxicity against B. Xylophilus (LC₅₀ = 8.52 μg/mL). The octanoic acid and (E)-2-decenal also exhibited moderate toxicity against B. xylophilus (LC₅₀ = 65.56 and 176.34 μg/mL, respectively). The LC₅₀ of falcarinol for the toxicity of B. xylophilus was 7.7 and 21 times than that of octanoic acid and (E)-2-decenal, respectively. Our findings demonstrate that the essential oil from Seseli mairei H. Wolff roots and their isolates may be developed as a promising natural nematicide.

The amalgamation of ideological and political theory into the significant courses in the curriculum offered by the educational institutions has gained more attention in recent years. The primary goal of this process is to enhance the learners’ comprehensive quality not only in knowledge education but also in ideological education. This work focuses on the study of integration of curriculum ideology and politics into the biostatistics course. In the section of data collection and sorting, calculation of data characteristic number and hypothesis testing, this paper introduces detailed analysis of specific problems and the main and secondary aspects of contradictions in the teaching content. At the same time, it also carries out the education of cultural self-confidence and integrates the ideological content of the curriculum into the teaching practice. This work also proposed questionnaire survey-based method to find the relationship between biostatistics course and ideological and political education.