In the present work, we have studied daily rhythmicity of body temperature (Tb) in Arabian camels challenged with daily heat, combined or not with dehydration. We confirm that Arabian camels use heterothermy to reduce heat gain coupled with evaporative heat loss during the day. Here, we also demonstrate that this mechanism is more complex than previously reported, because it is characterized by a daily alternation (probably of circadian origin) of two periods of poikilothermy and homeothermy. We also show that dehydration induced a decrease in food intake plays a role in this process. Together, these findings highlight that adaptive heterothermy in the Arabian camel varies across the diurnal light–dark cycle and is modulated by timing of daily heat and degrees of water restriction and associated reduction of food intake. The changed phase relationship between the light–dark cycle and the Tb rhythm observed during the dehydration process points to a possible mechanism of internal desynchronization during the process of adaptation to desert environment. During these experimental conditions mimicking the desert environment, it will be possible in the future to determine if induced high‐amplitude ambient temperature (Ta) rhythms are able to compete with the zeitgeber effect of the light–dark cycle.

In mammals the light-dark (LD) cycle is known to be the major cue to synchronize the circadian clock. In arid and desert areas, the camel ( Camelus dromedarius) is exposed to extreme environmental conditions. Since wide oscillations of ambient temperature (T_a) are a major factor in this environment, we wondered whether cyclic T_a fluctuations might contribute to synchronization of circadian rhythms. The rhythm of body temperature (T_b) was selected as output of the circadian clock. After having verified that T_b is synchronized by the LD and free runs in continuous darkness (DD), we submitted the animals to daily cycles of T_a in LL and in DD. In both cases, the T_b rhythm was entrained to the cycle of T_a. On a 12-h phase shift of the T_a cycle, the mean phase shift of the T_b cycle ranged from a few hours in LD (1 h by cosinor, 4 h from curve peaks) to 7–8 h in LL and 12 h in DD. These results may reflect either true synchronization of the central clock by T_a daily cycles or possibly a passive effect of T_a on T_b. To resolve the ambiguity, melatonin rhythmicity was used as another output of the clock. In DD melatonin rhythms were also entrained by the T_a cycle, proving that the daily T_a cycle is able to entrain the circadian clock of the camel similar to photoperiod. By contrast, in the presence of a LD cycle the rhythm of melatonin was modified by the T_a cycle in only 2 (or 3) of 7 camels: in these specific conditions a systematic effect of T_a on the clock could not be evidenced. In conclusion, depending on the experimental conditions (DD vs. LD), the daily T_a cycle can either act as a zeitgeber or not.

Dromedary camels are unique in their morphological and physiological characteristics and are capable of providing milk and meat even under extreme environmental conditions. Like other species, the dromedary camel has also benefitted from the development of the molecular genetics to increase the knowledge about different aspect in camel genetics (genetic variation, molecular marker, parentage control, gene of interest, whole genome, dating…etc.). In this paper we review the different molecular genetic technics used in this particular species and future prospects. Dromedary genetic studies started in the end of the 1980s with phenotypic evaluation and the attempts to highlight the protein and biochemical diversity. In the 2000s, with the development of molecular markers such as microsatellites, genetic diversity of different types in several countries were estimated and microsatellites were also used for parentage control. In terms of genetic characterization, microsatellites revealed a defined global structure, differentiating East African and South Arabian dromedaries from North African, North Arabian, and South Asian individuals, respectively. Also, mitochondrialDNA sequence analysis of ancient DNA proved to be crucial in resolving domestication processes in dromedaries. Ancient and modern DNA revealed dynamics of domestication and cross-continental dispersion of the dromedary. Nuclear SNPs, single nucleotide polymorphisms changes that occur approximately each 1000 bps in the mammalian genome were also applied in some studies in dromedary. These markers are a very useful alternative to microsatellites and have been employed in some studies on genetic diversity and relevant phenotypic traits in livestock. Finally, thanks to the use of Next Generation Sequencing (NGS) the whole-genome assemblies of the dromedary (Camelus dromedarius) and a work to establish the organization of the dromedary genome at chromosome level were recently published.

To evaluate the humoral immune response to mixed Equine Influenza vaccination, a common practice in the field, an experimental study was carried out on 42 unvaccinated thoroughbred weanling foals divided into six groups of seven. Three groups were vaccinated using a non-mixed protocol (Equilis^® Prequenza-Te, Proteqflu-Te^® or Calvenza-03^®) and three other groups were vaccinated using a mix of the three vaccines mentioned previously. Each weanling underwent a primary EI vaccination schedule composed of two primary immunisations (V1 and V2) four weeks apart followed by a third boost immunisation (V3) six months later. Antibody responses were monitored until one-year post-V3 by single radial haemolysis (SRH). The results showed similar antibody responses for all groups using mixed EI vaccination and the group exclusively vaccinated with Equilis^® Prequenza-TE, which were significantly higher than the other two groups vaccinated with Proteqflu-TE^® and Calvenza-03^®. All weanlings (100%) failed to seroconvert after V1 and 21% (9/42) still had low or no SRH antibody titres two weeks post-V2. All weanlings had seroconverted and exceeded the clinical protection threshold one month after V3. The poor response to vaccination was primarily observed in groups exclusively vaccinated with Proteqflu-Te^® and Calvenza-03^®. A large window of susceptibility (3–4.5-month duration) usually called immunity gap was observed after V2 and prior to V3 for all groups. The SRH antibody level was maintained above the clinical protection threshold for three months post-V3 for the groups exclusively vaccinated with Proteqflu-Te^® and Calvenza-03^®, and six months to one year for groups using mixed EI vaccination or exclusively vaccinated with Equilis^® Prequenza-Te. This study demonstrates for the first time that the mix of EI vaccines during the primary vaccination schedule has no detrimental impact on the correlate of protection against EIV infection.

Background: The locomotor activity (LA) rhythm, widely studied in rodents, has not been fully investigated in large mammals. This is due to the high cost and the brittleness of the required devices. Alternatively, the locomotion scoring method (SM), consisting of attribution of a score to various levels of activity would be a consistent method to assess the circadian LA rhythm in such species. New method: To test this, a SM with a score ranging from 0 to 5 has been developed and used in two domestic large mammals, the camel and the goat. One minute interval scoring was performed using visual screening and monitoring of infra-red camera recording videos and carried out by two evaluators. Results: The SM provides a clear daily LA rhythm that has been validated using an automate device, the Actiwatch-Mini. The obtained curves and actograms were indeed highly similar to those acquired from the Actiwatch-Mini. Moreover, there were no statistical differences in the period and acrophase. The period was exactly of 24.0h and the acrophases occurred at 12h05 +/- 00h03 and 12h14 +/- 00h07 for the camel and at 13h13 +/- 00h09 and 12h57 +/- 00h09 for the goat using SM and Actiwatch-Mini respectively. Comparison with existing methods: Compared to the automatic system, the SM is inexpensive and has the advantage of describing all types of performed movements. Conclusions: The new developed SM is highly reliable and sufficiently accurate to assess conveniently the LA rhythm and specific behaviors in large mammals. This opens new perspectives to study chronobiology in animal models of desert, tropical and equatorial zones.