Quinoa (Chenopodium quinoa Willd., Amaranthaceae) is an environmental stress-resilient crop of increasing global importance. Sowing density is a critical factor in the quinoa cultivation protocol. We evaluated the row-spacing effect on quinoa growth, yield, and grain quality under Mediterranean conditions. We hypothesized that lower row spacing would reduce quinoa stem diameter and increase yield but may reduce grain quality. Two quinoa accessions were sown in northern Israel with 16, 26, or 80 cm between rows during two consecutive years, in November and January each year. Plant density at harvest ranged from 22 to 260 plants m⁻². Plant height and stem diameter ranged from 77 to 126 and 6.3 to 10.5 cm, respectively. Hay, grain, and straw yield ranged from 2259 to 17,979, 1604 to 4266, and 1212 to 3660 kg DM ha⁻¹, respectively. Grain protein content (PC) ranged from 5.2 to 14.2 and thousand grain weight (TGW) from 2033 to 3446 mg. Plant density, hay, grain, and straw yield were negatively correlated to row spacing. Stem diameter was positively correlated to row spacing, while there were no correlations between this parameter and plant height, grain PC, or TGW. Results indicated that 16 cm between rows may be optimal, as this produced the greatest yields with no effect on grain quality. However, as it may result in plant lodging, 26 cm row spacing should also be considered. The effects of additional management-related parameters on quinoa production should be examined.

Chickpea (Cicer arietinum L.) is an important crop in crop-rotation management in Israel. Imidazolinone herbicides have a wide spectrum of weed control, but chickpea plants are sensitive to acetohydroxyacid synthase (AHAS; also known as acetolactate synthase [ALS]) inhibitors. Using the chemical mutagen ethyl methanesulfonate (EMS), we developed a chickpea line (M2033) that is resistant to imidazolinone herbicides. A point mutation was detected in one of the two genes encoding the AHAS catalytic subunit of M2033. The transition of threonine to isoleucine at position 192 (203 according to Arabidopsis) conferred resistance of M2033 to imidazolinones, but not to other groups of AHAS inhibitors. The role of this substitution in the resistance of line M2033 was proven by genetic transformation of tobacco plants. This resistance showed a single-gene semidominant inheritance pattern. Conclusion: A novel mutation, T192I (T203I according to Arabidopsis), providing resistance to IMI herbicides but not to other groups of AHAS inhibitors, is described in the AHAS1 protein of EMS-mutagenized chickpea line M2033.

Young vegetative quinoa (YVQ) has gained attention as a high-protein leafy crop for human consumption with potential for cultivation in Mediterranean and semiarid regions. We investigated the effects of inter-row spacing and genotype on YVQ fresh and dry matter (DM) yield, protein content (PC), and protein yield during summer cultivation in northern Israel in two separate, independent, randomized field experiments over two consecutive years (2020–2021). We hypothesized that row spacing and genotypic differences would significantly impact yield and PC. Inter-row spacing significantly affected plant density, ranging from 55 to 366 plants m⁻². Fresh and DM yields ranged from 4957 to 28,469 kg ha⁻¹ and 661 to 3737 kg DM ha⁻¹, respectively. PC ranged from 20.5 to 26.6% and was not significantly influenced by row spacing. Total protein yield ranged from 147 to 884 kg ha⁻¹. Among the five tested genotypes, no significant differences were observed in fresh (7477–17,776 kg ha⁻¹) or dry (1122–2199 kg DM ha⁻¹) biomass, PC (21.2–26.5%), or protein yield (260–579 kg ha⁻¹), suggesting limited genetic differentiation under the specific environmental and agronomic conditions tested. Amino acid analysis confirmed the presence of all nine essential amino acids, fulfilling over 30% of the recommended daily intake per 100 g DM. These findings highlight YVQ as a promising, sustainable, and protein-rich leafy crop for Mediterranean agriculture. Further research should explore multi-harvest potential, mechanical weeding, and optimized agronomic practices for commercial-scale production.

Tomato (Solanum lycopersicum L.) is extremely sensitive to inhibitors of acetohydroxyacid synthase (AHAS; also known as acetolactate synthase [ALS]). Utilizing ethyl methanesulfonate mutagenesis of seeds of the commercial tomato line ‘M82’, we developed a tomato mutant, HRT1, that showed high resistance to imidazolinone herbicides (which act by inhibiting AHAS) in the greenhouse and under field conditions. The activity of AHAS extracted from HRT1 was significantly less affected by imidazolinone herbicides than that from the parental line M82. Following imazapic treatment, no differences were found in the content of free branched-chain amino acids in HRT1 tissues as compared to a dramatic decrease in M82 tissues. No differences were found in the susceptibility of AHAS to sulfonylurea herbicides. A single point transition mutation of C to T in the AHAS1 gene located on chromosome 3 was detected. This mutation resulted in substitution of alanine by valine at amino acid position 194, corresponding to 205-Alal in Arabidopsis. Ligand–protein contact analysis showed that replacement of alanine by the larger hydrophobic valine residue results in increased repulsion, hindering herbicide binding. Segregation analysis indicated that the resistance to imidazolinones in line HRT1 is due to a single recessive gene.

Young vegetative quinoa (YVQ) has gained attention as a high-protein leafy crop for human consumption with potential for cultivation in Mediterranean and semiarid regions. We investigated the effects of inter-row spacing and genotype on YVQ fresh and dry matter (DM) yield, protein content (PC), and protein yield during summer cultivation in northern Israel in two separate, independent, randomized field experiments over two consecutive years (2020–2021). We hypothesized that row spacing and genotypic differences would significantly impact yield and PC. Inter-row spacing significantly affected plant density, ranging from 55–366 plants m ^–2. Fresh and DM yields ranged from 4,957–28,469 kg ha^–1 and 661–3,737 kg DM ha^–1, respectively. PC ranged from 20.5%–26.6% and was not significantly influenced by row spacing. Total protein yield ranged from 147–884 kg ha^–1. Among the five tested genotypes, no significant differences were observed in fresh (7,477–17,776 kg ha^–1) or dry (1,122–2,199 kg DM ha^–1) biomass, PC (21.2%–26.5%), or protein yield (260–579 kg ha^–1), suggesting limited genetic differentiation under the specific environmental and agronomic conditions tested. Amino acid analysis confirmed the presence of all nine essential amino acids, fulfilling over 30% of the recommended daily intake per 100 g DM. These findings highlight YVQ as a promising, sustainable, and protein-rich leafy crop for Mediterranean agriculture. Further research should explore multi-harvest potential, mechanical weeding, and optimized agronomic practices for commercial-scale production.