The food production system needs to be sustainable including poultry sector to feed the increasing global population. An accepted economical and environmental approach of broiler production is to produce larger broilers faster while using less feed. Broiler production is aimed at producing consumable meat and meat products. The global broiler meat market has evolved over the years with increasing selection pressure shifted toward attaining yield characteristics for increased cut-up parts such as breast and thighs. There is a shift toward a big bird market in the U.S. with approximately 70% of the broiler meat produced from large birds (>2.72 kg). Genetic selection of broilers for quantitative traits such as growth rate and lean muscle mass without increasing the fat mass has altered broiler physiological homeostasis to adapt toward the larger rates of muscle protein turnover. Physiological stresses created due to selection pressures in broilers have produced several muscle myopathies including an emerging one called woody breast myopathy. The sustainable broiler production practice may require humane consideration of raising broilers in less stressful grow-out regimes that will have minimal impact on broiler metabolic health. Another sustainability approach of broiler production toward feed efficiency lies on understanding dietary formulation approach of amino acids and energy that promote optimal nutrient utilization and minimal nutrient output to environment while also fulfilling the growth demands and body composition changes associated with increased protein gain in current meat broilers brought by the genetic progress.

The study of neurochemical-based interkingdom signaling and its impact on host-microbe interaction is called microbial endocrinology. Neurochemicals play a recognized role in determining bacterial colonization and interaction with the gut epithelium. While much attention has been devoted to the determination of neurochemical concentrations in the mammalian gut to better understand tissue and region-specific microbial endocrinology-based mechanisms of host-microbe interaction, little is known regarding the biogeography of neurochemicals in the avian gut. Greater resolution of avian gut neurochemical concentrations is needed especially as recent microbial endocrinology-based investigations into bacterial foodborne pathogen colonization of the chicken gut have demonstrated neurochemicals to affect Campylobacter jejuni and Salmonella spp. in vivo and in vitro. The aim of the present study was to determine the concentrations of stress-related neurochemicals in the tissue and luminal content of the duodenum, jejunum, ileum, cecum, and colon of the broiler intestinal tract, and to investigate if this biogeography changes with age of the bird. While all neurochemicals measured were detected in the intestinal tract, many displayed differences in regional concentrations. Whereas the catecholamine norepinephrine was detected in each region of the intestinal tract, epinephrine was present only in the cecum and colon. Likewise, dopamine, and its metabolite 3,4-dihydroxyphenylacetic acid were found in the greatest quantities in the cecum and colon. Serotonin and histamine were identified in each gut region. Region-specific age-related changes were observed (P < 0.05) for serotonin, its metabolite 5-hydroxyindole acetic acid as well as for histamine. Several neurochemicals, including norepinephrine, were found in the contents of each gut region. Epinephrine was not detected in the gut content of any region. Salsolinol, a microbial-produced neuroactive compound was detected in the gut content but not in tissue. Together, our data establish a neurochemical biogeography of the broiler chicken intestinal tract. By providing researchers with a region-by-region map of in vivo gut neurochemical concentrations of a modern broiler chicken breed, this neurochemical map is expected to inform future investigations that seek to utilize avian enteric neurochemistry.

Background and Objective: Several studies confirm that the age of hens has a tremendous impact on external and internal egg quality characteristics. Egg production could be at serious risk if egg quality characteristics and age of hens are not seriously considered. This study was conducted to analyze the phenotypic correlations between some internal and external egg quality characteristics in old laying hens. Materials and Methods: A total of 288 eggs of 85-week-old Hy-Line Brown laying hens were collected during 3 weeks and their internal and external egg characteristics were evaluated. Results: Phenotypic correlations between egg quality characteristics in old laying hens indicate a negative impact on shell and albumen quality but not affected yolk quality characteristics. Conclusion: This study helps to understand that raising laying hens above 80 weeks would have a negative impact on egg quality characteristics.

Two meat-type broiler lines, line A and line B were fed experimental diets from 22-42 d with objectives to determine the effects of dietary metabolizable energy (ME) levels on feed intake (FI), performance, body composition, and processing yield as affected by environmental grow-out temperatures. Two thousand fifty male chicks from line A and 2,050 male chicks from line B were reared in 90-floor pens, 45 chicks per pen utilizing primary breeder nutrition and husbandry guidelines for starter (1-10 d) and grower (11-21 d) phases. Experimental finisher diets consisted of 5 increasing levels of apparent nitrogen corrected ME (2,800, 2,925, 3,050, 3,175, and 3,300 kcal/kg set at 19.5% crude protein and 1.0% dLys at each level) to represent 80, 90, 100, 110, and 120% ME of Evonik AminoChick energy level giving 2 × 5 factorial design and were fed from 22-42 d. All other amino acid levels in diets were formulated to a fixed ratio of dLys level. There were nine replicate pens for each diet and each line. The experiment was conducted twice-once in hot season (barn averages: 77.55 ˚F and 86.04% RH) and another in cool season (barn averages: 69.91 ˚F and 63.98% RH) of the year. Results showed that FI and feed conversion ratios (FCR) decreased (P < 0.05) linearly (R2 = 0.9) by 61.25 g and 0.073 units for every 10% increase in dietary ME for combined analysis of lines and seasons. The % fat mass of total body mass increased by 0.57%, whereas % protein mass decreased by 0.21% across ME levels (R2 > 0.9). However, there was no difference (P > 0.05) in % weights (of live weight) for wings, breast filet, tenders, or leg quarters across ME levels for both lines except % fat pad that increased (P < 0.05) by 0.20% for each 10% increment in dietary ME level. Line B had higher cumulative FI, BW gain, % lean, and protein mass of body mass than line A in hot season (P < 0.05). Feed intake was not different between lines in cool season (P > 0.05), whereas higher BW and improved FCR were observed for line A. Line A had higher % fat mass in both seasons. In summary, performance and yield results as affected by dietary ME levels were line specific and were affected by grow-out seasons. The optimal dietary ME level for the ME range studied (2,800-3,000 kcal/kg) at a constant recommended amino acid level lies in determining the best performance and profitability indices by taking into account the grow-out production inputs and processing yield outputs.

Microbial endocrinology, which is the study of neurochemical-based host-microbe interaction, has demonstrated that neurochemicals affect bacterial pathogenicity. A variety of neurochemicals, including norepinephrine, were shown to enhance intestinal epithelial colonization by Campylobacter jejuni. Yet, little is known whether serotonin, an abundant neurochemical produced in the gut, affects the physiology of C. jejuni and its interaction with the host gut epithelium. Considering the avian gut produces serotonin and serves as a major reservoir of C. jejuni, we sought to investigate whether serotonin can affect C. jejuni physiology and gut epithelial colonization in vitro. We first determined the biogeographical distribution of serotonin concentrations in the serosa, mucosa, as well as the luminal contents of the broiler chicken ileum, cecum, and colon. Serotonin concentrations were greater (P < 0.05) in the mucosa and serosa compared to the luminal content in each gut region examined. Among the ileum, colon, and cecum, the colon was found to contain the greatest concentrations of serotonin. We then investigated whether serotonin may effect changes in C. jejuni growth and motility in vitro. The C. jejuni used in this study was previously isolated from the broiler chicken ceca. Serotonin at concentrations of 1mM or below did not elicit changes in growth (P > 0.05) or motility (P > 0.05) of C. jejuni. Next, we utilized liquid chromatography tandem mass spectrometry to investigate whether serotonin affected the proteome of C. jejuni. Serotonin caused (P < 0.05) the downregulation of a protein (CJJ81176_1037) previously identified to be essential in C. jejuni colonization. Based on our findings, we evaluated whether serotonin would cause a functional change in C. jejuni adhesion and invasion of the HT29MTX-E12 colonic epithelial cell line. Serotonin was found to cause a reduction in adhesion (P < 0.05) but not invasion (P > 0.05). Together, we have identified a potential role for serotonin in modulating C. jejuni colonization in the gut in vitro. Further studies are required to understand the practical implications of these findings for the control of C. jejuni enteric colonization in vivo.