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Cowpea [Vigna unguiculata (L.) Walp.] is a major legume crop and an important source of protein in Africa. The Kafr El-Sheikh University has a long history of cowpea breeding and improvement in Egypt. Two superior lines with high seed yield and quality were selected through mutation breeding and released to farmers as new varieties under the names Kafr El Sheikh-1 and Kaha-1. Crosses were made between these two varieties to further improve cowpea to meet farmers’ demand. Using the pedigree selection method, 13 new superior F10 lines were selected and evaluated over 2 years for seed yield and related traits, earliness, and protein content under low (16 plants/m2) and high (24 plants/m2) plant densities. The results showed that plants grown in narrower space produced significantly higher seed yield per unit area than the plants grown in wider space. All developed lines produced significantly higher seed yield than the two parental lines in the 2018 trial and Kaha-1 in the 2019 trial. Line number 6 proved to be the best genotype for earliness (73.5–73.9 days after sowing), seed yield (573–647 g/m2), and crude protein content (22.7–24.3%) in both trials. In addition, line 4 with bushy determinate growth habit and high seed quality was recently released as a new variety (Sakha-1). Several other cowpea lines have clear potential for release as new high-yielding varieties with early maturity and high seed quality for farmers in Egypt. Seeds of selected lines are available from Kafrelsheikh University. This shows that mutation breeding and pedigree selection methods are among the most promising breeding methods for cowpea improvement.
Elmahdy Metwally; Mohamed Sharshar; Ali Masoud; Ali Masry; Atef Fiad; Benjamin Kilian; Shivali Sharma; Paul D. Shaw; Sebastian Raubach; Mohamed Rakha. Development of High Yielding Cowpea [Vigna unguiculata (L.) Walp.] Lines with Improved Quality Seeds through Mutation and Pedigree Selection Methods. Horticulturae 2021, 7, 271 .
AMA StyleElmahdy Metwally, Mohamed Sharshar, Ali Masoud, Ali Masry, Atef Fiad, Benjamin Kilian, Shivali Sharma, Paul D. Shaw, Sebastian Raubach, Mohamed Rakha. Development of High Yielding Cowpea [Vigna unguiculata (L.) Walp.] Lines with Improved Quality Seeds through Mutation and Pedigree Selection Methods. Horticulturae. 2021; 7 (9):271.
Chicago/Turabian StyleElmahdy Metwally; Mohamed Sharshar; Ali Masoud; Ali Masry; Atef Fiad; Benjamin Kilian; Shivali Sharma; Paul D. Shaw; Sebastian Raubach; Mohamed Rakha. 2021. "Development of High Yielding Cowpea [Vigna unguiculata (L.) Walp.] Lines with Improved Quality Seeds through Mutation and Pedigree Selection Methods." Horticulturae 7, no. 9: 271.
Wheat yields are stagnating around the world and new sources of genes for resistance or tolerances to abiotic traits are required. In this context, the tetraploid wheat wild relatives are among the key candidates for wheat improvement. Despite of its potential huge value for wheat breeding, the tetraploid GGAtAt genepool is largely neglected. Understanding the population structure, native distribution range, intraspecific variation of the entire tetraploid GGAtAt genepool and its domestication history would further its use for wheat improvement. We report the first comprehensive survey of genomic and cytogenetic diversity sampling the full breadth and depth of the tetraploid GGAtAt genepool. We show that the extant GGAtAt genepool consists of three distinct lineages. We provide detailed insights into the cytogenetic composition of GGAtAt wheats, revealed group-, and population-specific markers and show that chromosomal rearrangements play an important role in intraspecific diversity of T. araraticum. We discuss the origin and domestication history of the GGAtAt lineages in the context of state-of-the-art archaeobotanical finds. We shed new light on the complex evolutionary history of the GGAtAt wheat genepool. We provide the basis for an increased use of the GGAtAt wheat genepool for wheat improvement. The findings have implications for our understanding of the origins of agriculture in southwest Asia.
Ekaterina D. Badaeva; Fedor A. Konovalov; Helmut Knüpffer; Agostino Fricano; Alevtina S. Ruban; Zakaria Kehel; Svyatoslav A. Zoshchuk; Sergei A. Surzhikov; Kerstin Neumann; Andreas Graner; Karl Hammer; Anna Filatenko; Amy Bogaard; Glynis Jones; Hakan Özkan; Benjamin Kilian. Genetic diversity, distribution and domestication history of the neglected GGAtAt genepool of wheat. 2021, 1 .
AMA StyleEkaterina D. Badaeva, Fedor A. Konovalov, Helmut Knüpffer, Agostino Fricano, Alevtina S. Ruban, Zakaria Kehel, Svyatoslav A. Zoshchuk, Sergei A. Surzhikov, Kerstin Neumann, Andreas Graner, Karl Hammer, Anna Filatenko, Amy Bogaard, Glynis Jones, Hakan Özkan, Benjamin Kilian. Genetic diversity, distribution and domestication history of the neglected GGAtAt genepool of wheat. . 2021; ():1.
Chicago/Turabian StyleEkaterina D. Badaeva; Fedor A. Konovalov; Helmut Knüpffer; Agostino Fricano; Alevtina S. Ruban; Zakaria Kehel; Svyatoslav A. Zoshchuk; Sergei A. Surzhikov; Kerstin Neumann; Andreas Graner; Karl Hammer; Anna Filatenko; Amy Bogaard; Glynis Jones; Hakan Özkan; Benjamin Kilian. 2021. "Genetic diversity, distribution and domestication history of the neglected GGAtAt genepool of wheat." , no. : 1.
Benjamin Kilian; Hannes Dempewolf; Luigi Guarino; Peter Werner; Clarice Coyne; Marilyn L. Warburton. Crop Science special issue: Adapting agriculture to climate change: A walk on the wild side. Crop Science 2020, 61, 32 -36.
AMA StyleBenjamin Kilian, Hannes Dempewolf, Luigi Guarino, Peter Werner, Clarice Coyne, Marilyn L. Warburton. Crop Science special issue: Adapting agriculture to climate change: A walk on the wild side. Crop Science. 2020; 61 (1):32-36.
Chicago/Turabian StyleBenjamin Kilian; Hannes Dempewolf; Luigi Guarino; Peter Werner; Clarice Coyne; Marilyn L. Warburton. 2020. "Crop Science special issue: Adapting agriculture to climate change: A walk on the wild side." Crop Science 61, no. 1: 32-36.
Climate change is affecting agricultural production in the coastal areas of the Mekong Delta through the intrusion of salinity into the rice fields, where farmers cultivate photoperiod‐sensitive rice varieties with long growth duration and low grain yields. A set of 12 stable Crop Wild Relative (CWR)‐derived rice lines with introgressions from wild rice Oryza rufipogon and O. nivara was evaluated for their phenotypic response to salinity tolerance by a participatory selection approach on farm in the Phuoc Long and Gia Rai districts, Bac Lieu province. The evaluation of the results showed that four lines derived from CWR are well adapted to the local environmental conditions, with high grain yield (>6.5 tons/ha), early maturity, and short plant height. These CWR‐derived lines were adopted by farmers and proposed for testing on a larger scale in different areas of the coastal zone. This article is protected by copyright. All rights reserved
Huynh Quang Tin; Nguyen Huu Loi; Åsmund Bjornstad; Benjamin Kilian. Participatory selection of CWR‐derived salt‐tolerant rice lines adapted to the coastal zone of the Mekong Delta. Crop Science 2020, 61, 277 -288.
AMA StyleHuynh Quang Tin, Nguyen Huu Loi, Åsmund Bjornstad, Benjamin Kilian. Participatory selection of CWR‐derived salt‐tolerant rice lines adapted to the coastal zone of the Mekong Delta. Crop Science. 2020; 61 (1):277-288.
Chicago/Turabian StyleHuynh Quang Tin; Nguyen Huu Loi; Åsmund Bjornstad; Benjamin Kilian. 2020. "Participatory selection of CWR‐derived salt‐tolerant rice lines adapted to the coastal zone of the Mekong Delta." Crop Science 61, no. 1: 277-288.
Breeding for salt tolerance or abiotic stress, in general, requires rapid but reliable screening protocols that reflect the actual field situation as much as possible. A collection of 200 CWR‐derived BC3F3‐4 rice (Oryza sativa L.) lines developed at IRRI were evaluated by farmers in the Mekong Delta over two seasons for agronomic performance. Fifty stable BC3F5 lines were selected and subsequently screened in hydroponics using three NaCl concentrations to assess their phenotypic response to salt stress. The lines and check varieties were grown in a salinized Yoshida nutrient solution at three concentrations: 68 mM, 102 mM, and 136 mM NaCl. Several lines were identified to be tolerant to salinity stress and ANOVA showed significant differences among Genotypes and NaCl concentrations. Root and shoot growth parameters showed an inverse relationship with increasing NaCl concentration. Population genetic analysis suggested four groups of genotypes, where the median salt injury score across the three NaCl concentrations was identified as the main clustering factor. Lines from Cluster 3 were identified as the most promising donors of salt tolerance. This article is protected by copyright. All rights reserved
Huynh Quang Tin; Nguyen Huu Loi; Sandy Jan E. Labarosa; Kenneth L. McNally; Susan McCouch; Benjamin Kilian. Phenotypic response of farmer‐selected CWR‐derived rice lines to salt stress in the Mekong Delta. Crop Science 2020, 61, 201 -218.
AMA StyleHuynh Quang Tin, Nguyen Huu Loi, Sandy Jan E. Labarosa, Kenneth L. McNally, Susan McCouch, Benjamin Kilian. Phenotypic response of farmer‐selected CWR‐derived rice lines to salt stress in the Mekong Delta. Crop Science. 2020; 61 (1):201-218.
Chicago/Turabian StyleHuynh Quang Tin; Nguyen Huu Loi; Sandy Jan E. Labarosa; Kenneth L. McNally; Susan McCouch; Benjamin Kilian. 2020. "Phenotypic response of farmer‐selected CWR‐derived rice lines to salt stress in the Mekong Delta." Crop Science 61, no. 1: 201-218.
Changing climates and associated increased variability pose risks to alfalfa cultivation, with the requirement to establish, survive and maintain production under water stress. Crop wild relatives (CWR) of alfalfa include populations that have evolved to survive in a number of different, extreme environments, but until recently have had limited use in breeding programs. Here we report on the phenotypic diversity of alfalfa crop wild relatives (Medicago sativa ) that were selected to represent extremes in drought tolerance (by sourcing germplasm from environments with extremes in low rainfall, high temperature, shallow soils and winter freezing) with the aim of providing germplasm with drought tolerance and improved forage yield traits for breeding programs in both warm and cool dry temperate environments. Newly formed hybrids created between M. sativa , M. arborea (a woody shrub) and M. truncatula (an annual species from the Mediterranean region) were developed or acquired to introduce new genetic diversity from the tertiary genepool. Preliminary characterization and evaluation was used for taxonomic classification, and to identify wild accessions and pre‐bred (hybrid) lines that offer new diversity for growth habit, seed size, fall dormancy and forage yield. The accessions and pre‐breeding lines described have been donated to the Australian Pastures Genebank for conservation and distribution. This article is protected by copyright. All rights reserved
Alan W. Humphries; Carlos Ovalle; Steve Hughes; Alejandro Del Pozo; Luis Inostroza; Viviana Barahona; Linqing Yu; Sakysh Yerzhanova; Trevor Rowe; Jeff Hill; Galiolla Meiirman; Serik Abayev; E. Charles Brummer; David M. Peck; Saltanat Toktarbekova; Bauyrzhan Kalibayev; Soledad Espinoza; Jorge Ivelic‐Saez; Edwin Bingham; Ernest Small; Benjamin Kilian. Characterization and pre‐breeding of diverse alfalfa wild relatives originating from drought‐stressed environments. Crop Science 2020, 61, 69 -88.
AMA StyleAlan W. Humphries, Carlos Ovalle, Steve Hughes, Alejandro Del Pozo, Luis Inostroza, Viviana Barahona, Linqing Yu, Sakysh Yerzhanova, Trevor Rowe, Jeff Hill, Galiolla Meiirman, Serik Abayev, E. Charles Brummer, David M. Peck, Saltanat Toktarbekova, Bauyrzhan Kalibayev, Soledad Espinoza, Jorge Ivelic‐Saez, Edwin Bingham, Ernest Small, Benjamin Kilian. Characterization and pre‐breeding of diverse alfalfa wild relatives originating from drought‐stressed environments. Crop Science. 2020; 61 (1):69-88.
Chicago/Turabian StyleAlan W. Humphries; Carlos Ovalle; Steve Hughes; Alejandro Del Pozo; Luis Inostroza; Viviana Barahona; Linqing Yu; Sakysh Yerzhanova; Trevor Rowe; Jeff Hill; Galiolla Meiirman; Serik Abayev; E. Charles Brummer; David M. Peck; Saltanat Toktarbekova; Bauyrzhan Kalibayev; Soledad Espinoza; Jorge Ivelic‐Saez; Edwin Bingham; Ernest Small; Benjamin Kilian. 2020. "Characterization and pre‐breeding of diverse alfalfa wild relatives originating from drought‐stressed environments." Crop Science 61, no. 1: 69-88.
In barley, the transition from the vegetative to reproductive phase is complex and under the control of photoperiodic and temperature conditions. One major gene involved isPPD-H1, aPSEUDO-RESPONSE REGULATOR 7(PRR7) that encodes a component of the circadian clock. Mutation atPPD-H1resulted in the photoperiod non-responsiveppd-H1alleles that are beneficial under high latitudinal environments as they allow vegetative growth during the long-day summer conditions whereby higher yields are harvested by farmers. Utilizing a diverse GWAS panel of world-wide origin and a genome-wide gene-based set of 50K SNP markers, a strong association of days to heading with thePPD-H1gene was detected in multi-location field trials. Re-sequencing of the gene spanning putative causative SNPs, SNP22 (Turner et al. 2005) and SNP48 (Jones et al. 2008), detected recombination between the two, previously reported to be in complete LD. Phenotyping of the recombinants and phylogenetic relationships among haplotypes supported the original conclusion of Turner et al. (2005) that SNP22, present in the CCT domain, is the most likely causative SNP. To infer the origin of non-responsiveness, thePPD-H1gene was re-sequenced in a geo-referenced collection of 2057 wild and domesticated barleys and compared with the allelic status of the 6000-year-old barley sample from the Yoram cave in the Masada cliff. A monophyletic and post-domestication origin in the Fertile Crescent was found in contrast to the pre-domestication origin proposed by Jones et al. (2008). We show that the photoperiod non-responsiveness originated from Desert type wild barley in the Southern Levant.
Rajiv Sharma; Salar Shaaf; Kerstin Neumann; Yu Guo; Martin Mascher; Michal David; Adnan Al-Yassin; Hakan Özkan; Tom Blake; Sariel Hübner; Nora P Castañeda-Álvarez; Stefania Grando; Salvatore Ceccarelli; Michael Baum; Andreas Graner; George Coupland; Klaus Pillen; Ehud Weiss; Ian J Mackay; Wayne Powell; Benjamin Kilian. On the origin of photoperiod non-responsiveness in barley. 2020, 1 .
AMA StyleRajiv Sharma, Salar Shaaf, Kerstin Neumann, Yu Guo, Martin Mascher, Michal David, Adnan Al-Yassin, Hakan Özkan, Tom Blake, Sariel Hübner, Nora P Castañeda-Álvarez, Stefania Grando, Salvatore Ceccarelli, Michael Baum, Andreas Graner, George Coupland, Klaus Pillen, Ehud Weiss, Ian J Mackay, Wayne Powell, Benjamin Kilian. On the origin of photoperiod non-responsiveness in barley. . 2020; ():1.
Chicago/Turabian StyleRajiv Sharma; Salar Shaaf; Kerstin Neumann; Yu Guo; Martin Mascher; Michal David; Adnan Al-Yassin; Hakan Özkan; Tom Blake; Sariel Hübner; Nora P Castañeda-Álvarez; Stefania Grando; Salvatore Ceccarelli; Michael Baum; Andreas Graner; George Coupland; Klaus Pillen; Ehud Weiss; Ian J Mackay; Wayne Powell; Benjamin Kilian. 2020. "On the origin of photoperiod non-responsiveness in barley." , no. : 1.
Many conflicting hypotheses regarding the relationships among crops and wild species closely related to wheat (the genera Aegilops, Amblyopyrum, and Triticum) have been postulated. The contribution of hybridization to the evolution of these taxa is intensely discussed. To determine possible causes for this, and provide a phylogeny of the diploid taxa based on genome‐wide sequence information, independent data was obtained from genotyping‐by‐sequencing and a target‐enrichment experiment that returned 244 low‐copy nuclear loci. The data were analyzed with Bayesian, likelihood and coalescent‐based methods. D statistics were used to test if incomplete lineage sorting alone or together with hybridization is the source for incongruent gene trees. Here we present the phylogeny of all diploid species of the wheat wild relatives. We hypothesize that most of the wheat‐group species were shaped by a primordial homoploid hybrid speciation event involving the ancestral Triticum and Am. muticum lineages to form all other species but Ae. speltoides. This hybridization event was followed by multiple introgressions affecting all taxa but Triticum. Mostly progenitors of the extant species were involved in these processes, while recent interspecific gene flow seems insignificant. The composite nature of many genomes of wheat‐group taxa results in complicated patterns of diploid contributions when these lineages are involved in polyploid formation, which is, for example, the case in the tetra‐ and hexaploid wheats. Our analysis provides phylogenetic relationships and a testable hypothesis for the genome compositions in the basic evolutionary units within the wheat group of Triticeae.
Nadine Bernhardt; Jonathan Brassac; Xue Dong; Eva‐Maria Willing; C. Hart Poskar; Benjamin Kilian; Frank R. Blattner. Genome‐wide sequence information reveals recurrent hybridization among diploid wheat wild relatives. The Plant Journal 2019, 102, 493 -506.
AMA StyleNadine Bernhardt, Jonathan Brassac, Xue Dong, Eva‐Maria Willing, C. Hart Poskar, Benjamin Kilian, Frank R. Blattner. Genome‐wide sequence information reveals recurrent hybridization among diploid wheat wild relatives. The Plant Journal. 2019; 102 (3):493-506.
Chicago/Turabian StyleNadine Bernhardt; Jonathan Brassac; Xue Dong; Eva‐Maria Willing; C. Hart Poskar; Benjamin Kilian; Frank R. Blattner. 2019. "Genome‐wide sequence information reveals recurrent hybridization among diploid wheat wild relatives." The Plant Journal 102, no. 3: 493-506.
Genebanks are valuable sources of genetic diversity, which can help to cope with future problems of global food security caused by a continuously growing population, stagnating yields and climate change. However, the scarcity of phenotypic and genotypic characterization of genebank accessions severely restricts their use in plant breeding. To warrant the seed integrity of individual accessions during periodical regeneration cycles in the field phenotypic characterizations are performed. This study provides non-orthogonal historical data of 12,754 spring and winter wheat accessions characterized for flowering time, plant height, and thousand grain weight during 70 years of seed regeneration at the German genebank. Supported by historical weather observations outliers were removed following a previously described quality assessment pipeline. In this way, ready-to-use processed phenotypic data across regeneration years were generated and further validated. We encourage international and national genebanks to increase their efforts to transform into bio-digital resource centers. A first important step could consist in unlocking their historical data treasures that allows an educated choice of accessions by scientists and breeders.
Norman Philipp; Stephan Weise; Markus Oppermann; Andreas Börner; Jens Keilwagen; Benjamin Kilian; Daniel Arend; Yusheng Zhao; Andreas Graner; Jochen C. Reif; Albert W. Schulthess. Historical phenotypic data from seven decades of seed regeneration in a wheat ex situ collection. Scientific Data 2019, 6, 137 .
AMA StyleNorman Philipp, Stephan Weise, Markus Oppermann, Andreas Börner, Jens Keilwagen, Benjamin Kilian, Daniel Arend, Yusheng Zhao, Andreas Graner, Jochen C. Reif, Albert W. Schulthess. Historical phenotypic data from seven decades of seed regeneration in a wheat ex situ collection. Scientific Data. 2019; 6 (1):137.
Chicago/Turabian StyleNorman Philipp; Stephan Weise; Markus Oppermann; Andreas Börner; Jens Keilwagen; Benjamin Kilian; Daniel Arend; Yusheng Zhao; Andreas Graner; Jochen C. Reif; Albert W. Schulthess. 2019. "Historical phenotypic data from seven decades of seed regeneration in a wheat ex situ collection." Scientific Data 6, no. 1: 137.
Many conflicting hypotheses regarding the relationships among crops and wild species closely related to wheat (the genera Aegilops, Amblyopyrum, and Triticum) have been postulated. The contribution of hybridization to the evolution of these taxa is intensely discussed. To determine possible causes for this, and provide a phylogeny of the diploid taxa based on genome-wide sequence information, independent data was obtained from genotyping-by-sequencing and a target-enrichment experiment that returned 244 low-copy nuclear loci. The data were analyzed with Bayesian, likelihood and coalescent-based methods. D statistics were used to test if incomplete lineage sorting alone or together with hybridization is the source for incongruent gene trees. Here we present the phylogeny of all diploid species of the wheat wild relatives. We hypothesize that most of the wheat-group species were shaped by a primordial homoploid hybrid speciation event involving the ancestral Triticum and Am. muticum lineages to form all other species but Ae. speltoides. This hybridization event was followed by multiple introgressions affecting all taxa but Triticum. Mostly progenitors of the extant species were involved in these processes, while recent interspecific gene flow seems insignificant. The composite nature of many genomes of wheat group taxa results in complicated patterns of diploid contributions when these lineages are involved in polyploid formation, which is, for example, the case in the tetra-and hexaploid wheats. Our analysis provides phylogenetic relationships and a testable hypothesis for the genome compositions in the basic evolutionary units within the wheat group of Triticeae.
Nadine Bernhardt; Jonathan Brassac; Xue Dong; Eva-Maria Willing; C. Hart Poskar; Benjamin Kilian; Frank R. Blattner. Genome-wide sequence information reveals recurrent hybridization among diploid wheat wild relatives. 2019, 678045 .
AMA StyleNadine Bernhardt, Jonathan Brassac, Xue Dong, Eva-Maria Willing, C. Hart Poskar, Benjamin Kilian, Frank R. Blattner. Genome-wide sequence information reveals recurrent hybridization among diploid wheat wild relatives. . 2019; ():678045.
Chicago/Turabian StyleNadine Bernhardt; Jonathan Brassac; Xue Dong; Eva-Maria Willing; C. Hart Poskar; Benjamin Kilian; Frank R. Blattner. 2019. "Genome-wide sequence information reveals recurrent hybridization among diploid wheat wild relatives." , no. : 678045.
Adaptation to drought-prone environments requires robust root architecture. Genotypes with a more vigorous root system have the potential to better adapt to soils with limited moisture content. However, root architecture is complex at both, phenotypic and genetic level. Customized mapping panels in combination with efficient screenings methods can resolve the underlying genetic factors of root traits. A mapping panel of 233 spring barley genotypes was evaluated for root and shoot architecture traits under non-stress and osmotic stress. A genome-wide association study elucidated 65 involved genomic regions. Among them were 34 root-specific loci, eleven hotspots with associations to up to eight traits and twelve stress-specific loci. A list of candidate genes was established based on educated guess. Selected genes were tested for associated polymorphisms. By this, 14 genes were identified as promising candidates, ten remained suggestive and 15 were rejected. The data support the important role of flowering time genes, including HvPpd-H1, HvCry2, HvCO4 and HvPRR73. Moreover, seven root-related genes, HERK2, HvARF04, HvEXPB1, PIN5, PIN7, PME5 and WOX5 are confirmed as promising candidates. For the QTL with the highest allelic effect for root thickness and plant biomass a homologue of the Arabidopsis Trx-m3 was revealed as the most promising candidate. This study provides a catalogue of hotspots for seedling growth, root and stress-specific genomic regions along with candidate genes for future potential incorporation in breeding attempts for enhanced yield potential, particularly in drought-prone environments. Root architecture is under polygenic control. The co-localization of well-known major genes for barley development and flowering time with QTL hotspots highlights their importance for seedling growth. Association analysis revealed the involvement of HvPpd-H1 in the development of the root system. The co-localization of root QTL with HERK2, HvARF04, HvEXPB1, PIN5, PIN7, PME5 and WOX5 represents a starting point to explore the roles of these genes in barley. Accordingly, the genes HvHOX2, HsfA2b, HvHAK2, and Dhn9, known to be involved in abiotic stress response, were located within stress-specific QTL regions and await future validation.
Adel H. Abdel-Ghani; Rajiv Sharma; Celestine Wabila; Sidram Dhanagond; Saed J. Owais; Mahmud A. Duwayri; Saddam A. Al-Dalain; Christian Klukas; Dijun Chen; Thomas Lübberstedt; Nicolaus Von Wirén; Andreas Graner; Benjamin Kilian; Kerstin Neumann. Genome-wide association mapping in a diverse spring barley collection reveals the presence of QTL hotspots and candidate genes for root and shoot architecture traits at seedling stage. BMC Plant Biology 2019, 19, 216 .
AMA StyleAdel H. Abdel-Ghani, Rajiv Sharma, Celestine Wabila, Sidram Dhanagond, Saed J. Owais, Mahmud A. Duwayri, Saddam A. Al-Dalain, Christian Klukas, Dijun Chen, Thomas Lübberstedt, Nicolaus Von Wirén, Andreas Graner, Benjamin Kilian, Kerstin Neumann. Genome-wide association mapping in a diverse spring barley collection reveals the presence of QTL hotspots and candidate genes for root and shoot architecture traits at seedling stage. BMC Plant Biology. 2019; 19 (1):216.
Chicago/Turabian StyleAdel H. Abdel-Ghani; Rajiv Sharma; Celestine Wabila; Sidram Dhanagond; Saed J. Owais; Mahmud A. Duwayri; Saddam A. Al-Dalain; Christian Klukas; Dijun Chen; Thomas Lübberstedt; Nicolaus Von Wirén; Andreas Graner; Benjamin Kilian; Kerstin Neumann. 2019. "Genome-wide association mapping in a diverse spring barley collection reveals the presence of QTL hotspots and candidate genes for root and shoot architecture traits at seedling stage." BMC Plant Biology 19, no. 1: 216.
Broadening the genetic base of crops is crucial for developing varieties to respond to global agricultural challenges such as climate change. Here, we analysed a diverse panel of 371 domesticated lines of the model crop of barley to explore the genetics of crop adaptation. We first collected exome sequence data and phenotypes of key life history traits from contrasting multi‐environment common garden trials. Then we applied refined statistical methods, including based on exomic haplotype states, for genotype‐by‐environment (G×E) modelling. Sub‐populations defined from exomic profiles were coincident with barley's biology, geography and history, and explained a high proportion of trial phenotypic variance. Clear G×E interactions indicated adaptation profiles that varied for landraces and cultivars. Exploration of circadian clock‐related genes, associated with the environmentally‐adaptive days to heading trait (crucial for the crop's spread from the Fertile Crescent), illustrated complexities in G×E effect directions, and the importance of latitudinally‐based genic context in the expression of large effect alleles. Our analysis supports a gene‐level scientific understanding of crop adaption and leads to practical opportunities for crop improvement, allowing the prioritisation of genomic regions and particular sets of lines for breeding efforts seeking to cope with climate change and other stresses.
Daniela Bustos‐Korts; Ian K. Dawson; Joanne Russell; Alessandro Tondelli; Davide Guerra; Chiara Ferrandi; Francesco Strozzi; Ezequiel L. Nicolazzi; Marta Molnar‐Lang; Hakan Ozkan; Maria Megyeri; Peter Miko; Esra Çakır; Enes Yakışır; Noemi Trabanco; Stefano Delbono; Stylianos Kyriakidis; Allan Booth; Davide Cammarano; Martin Mascher; Peter Werner; Luigi Cattivelli; Laura Rossini; Nils Stein; Benjamin Kilian; Robbie Waugh; Fred A. Van Eeuwijk. Exome sequences and multi‐environment field trials elucidate the genetic basis of adaptation in barley. The Plant Journal 2019, 99, 1172 -1191.
AMA StyleDaniela Bustos‐Korts, Ian K. Dawson, Joanne Russell, Alessandro Tondelli, Davide Guerra, Chiara Ferrandi, Francesco Strozzi, Ezequiel L. Nicolazzi, Marta Molnar‐Lang, Hakan Ozkan, Maria Megyeri, Peter Miko, Esra Çakır, Enes Yakışır, Noemi Trabanco, Stefano Delbono, Stylianos Kyriakidis, Allan Booth, Davide Cammarano, Martin Mascher, Peter Werner, Luigi Cattivelli, Laura Rossini, Nils Stein, Benjamin Kilian, Robbie Waugh, Fred A. Van Eeuwijk. Exome sequences and multi‐environment field trials elucidate the genetic basis of adaptation in barley. The Plant Journal. 2019; 99 (6):1172-1191.
Chicago/Turabian StyleDaniela Bustos‐Korts; Ian K. Dawson; Joanne Russell; Alessandro Tondelli; Davide Guerra; Chiara Ferrandi; Francesco Strozzi; Ezequiel L. Nicolazzi; Marta Molnar‐Lang; Hakan Ozkan; Maria Megyeri; Peter Miko; Esra Çakır; Enes Yakışır; Noemi Trabanco; Stefano Delbono; Stylianos Kyriakidis; Allan Booth; Davide Cammarano; Martin Mascher; Peter Werner; Luigi Cattivelli; Laura Rossini; Nils Stein; Benjamin Kilian; Robbie Waugh; Fred A. Van Eeuwijk. 2019. "Exome sequences and multi‐environment field trials elucidate the genetic basis of adaptation in barley." The Plant Journal 99, no. 6: 1172-1191.
For more than 10,000 years, the selection of plant and animal traits that are better tailored for human use has shaped the development of civilizations. During this period, bread wheat (Triticum aestivum) emerged as one of the world’s most important crops. We use exome sequencing of a worldwide panel of almost 500 genotypes selected from across the geographical range of the wheat species complex to explore how 10,000 years of hybridization, selection, adaptation and plant breeding has shaped the genetic makeup of modern bread wheats. We observe considerable genetic variation at the genic, chromosomal and subgenomic levels, and use this information to decipher the likely origins of modern day wheats, the consequences of range expansion and the allelic variants selected since its domestication. Our data support a reconciled model of wheat evolution and provide novel avenues for future breeding improvement.
Caroline Pont; Wheat and Barley Legacy for Breeding Improvement (WHEALBI) consortium; Thibault Leroy; Michael Seidel; Alessandro Tondelli; Wandrille Duchemin; David Armisen; Daniel Lang; Daniela Bustos-Korts; Nadia Goué; François Balfourier; Márta Molnár-Láng; Jacob Lage; Benjamin Kilian; Hakan Özkan; Darren Waite; Sarah Dyer; Thomas Letellier; Michael Alaux; Joanne Russell; Beat Keller; Fred Van Eeuwijk; Manuel Spannagl; Klaus F. X. Mayer; Robbie Waugh; Nils Stein; Luigi Cattivelli; Georg Haberer; Gilles Charmet; Jérôme Salse. Tracing the ancestry of modern bread wheats. Nature Structural Biology 2019, 51, 905 -911.
AMA StyleCaroline Pont, Wheat and Barley Legacy for Breeding Improvement (WHEALBI) consortium, Thibault Leroy, Michael Seidel, Alessandro Tondelli, Wandrille Duchemin, David Armisen, Daniel Lang, Daniela Bustos-Korts, Nadia Goué, François Balfourier, Márta Molnár-Láng, Jacob Lage, Benjamin Kilian, Hakan Özkan, Darren Waite, Sarah Dyer, Thomas Letellier, Michael Alaux, Joanne Russell, Beat Keller, Fred Van Eeuwijk, Manuel Spannagl, Klaus F. X. Mayer, Robbie Waugh, Nils Stein, Luigi Cattivelli, Georg Haberer, Gilles Charmet, Jérôme Salse. Tracing the ancestry of modern bread wheats. Nature Structural Biology. 2019; 51 (5):905-911.
Chicago/Turabian StyleCaroline Pont; Wheat and Barley Legacy for Breeding Improvement (WHEALBI) consortium; Thibault Leroy; Michael Seidel; Alessandro Tondelli; Wandrille Duchemin; David Armisen; Daniel Lang; Daniela Bustos-Korts; Nadia Goué; François Balfourier; Márta Molnár-Láng; Jacob Lage; Benjamin Kilian; Hakan Özkan; Darren Waite; Sarah Dyer; Thomas Letellier; Michael Alaux; Joanne Russell; Beat Keller; Fred Van Eeuwijk; Manuel Spannagl; Klaus F. X. Mayer; Robbie Waugh; Nils Stein; Luigi Cattivelli; Georg Haberer; Gilles Charmet; Jérôme Salse. 2019. "Tracing the ancestry of modern bread wheats." Nature Structural Biology 51, no. 5: 905-911.
Phylogenetically‐related groups of species contain lineage‐specific genes, which exhibit no sequence similarity to any genes outside of the lineage. We describe here that the Jekyll gene, required for sexual reproduction, exists in two much diverged allelic variants, Jek1 and Jek3. Despite low similarity, the Jek1 and Jek3 proteins share identical signal peptides, conserved cysteine positions and direct repeats. The Jek1/Jek3 sequences are located at the same chromosomal locus and inherited by monogenic Mendelian fashion. Jek3 has similar expression as Jek1 and complements the Jek1 function in Jek1‐defficient plants. Jek1 and Jek3 allelic variants were almost equally distributed in a collection of 485 wild and domesticated barley accessions. All domesticated barleys harboring the Jek1 allele belong to single haplotype J1‐H1 indicating a genetic bottleneck during domestication. Domesticated barleys harboring the Jek3 allele consisted of three haplotypes. Jekyll‐like sequences were found only in species of the closely related tribes Bromeae and Triticeae but not in other Poaceae. Non‐invasive magnetic resonance imaging revealed intrinsic grain structure in Triticeae and Bromeae, associated with the Jekyll function. The emergence of Jekyll suggests its role in separation of the Bromeae and Triticeae lineages within the Poaceae and identifies the Jekyll genes as lineage‐specific. This article is protected by copyright. All rights reserved.
Volodymyr Radchuk; Rajiv Sharma; Elena Potokina; Ruslana Radchuk; Diana Weier; Eberhard Munz; Miriam Schreiber; Martin Mascher; Nils Stein; Thomas Wicker; Benjamin Kilian; Ljudmilla Borisjuk. The highly divergent Jekyll genes, required for sexual reproduction, are lineage specific for the related grass tribes Triticeae and Bromeae. The Plant Journal 2019, 98, 961 -974.
AMA StyleVolodymyr Radchuk, Rajiv Sharma, Elena Potokina, Ruslana Radchuk, Diana Weier, Eberhard Munz, Miriam Schreiber, Martin Mascher, Nils Stein, Thomas Wicker, Benjamin Kilian, Ljudmilla Borisjuk. The highly divergent Jekyll genes, required for sexual reproduction, are lineage specific for the related grass tribes Triticeae and Bromeae. The Plant Journal. 2019; 98 (6):961-974.
Chicago/Turabian StyleVolodymyr Radchuk; Rajiv Sharma; Elena Potokina; Ruslana Radchuk; Diana Weier; Eberhard Munz; Miriam Schreiber; Martin Mascher; Nils Stein; Thomas Wicker; Benjamin Kilian; Ljudmilla Borisjuk. 2019. "The highly divergent Jekyll genes, required for sexual reproduction, are lineage specific for the related grass tribes Triticeae and Bromeae." The Plant Journal 98, no. 6: 961-974.
The domestication of wild emmer wheat led to the selection of modern durum wheat, grown mainly for pasta production. We describe the 10.45 gigabase (Gb) assembly of the genome of durum wheat cultivar Svevo. The assembly enabled genome-wide genetic diversity analyses revealing the changes imposed by thousands of years of empirical selection and breeding. Regions exhibiting strong signatures of genetic divergence associated with domestication and breeding were widespread in the genome with several major diversity losses in the pericentromeric regions. A locus on chromosome 5B carries a gene encoding a metal transporter (TdHMA3-B1) with a non-functional variant causing high accumulation of cadmium in grain. The high-cadmium allele, widespread among durum cultivars but undetected in wild emmer accessions, increased in frequency from domesticated emmer to modern durum wheat. The rapid cloning of TdHMA3-B1 rescues a wild beneficial allele and demonstrates the practical use of the Svevo genome for wheat improvement.
Marco Maccaferri; Neil S. Harris; Sven O. Twardziok; Raj K. Pasam; Heidrun Gundlach; Manuel Spannagl; Danara Ormanbekova; Thomas Lux; Verena M. Prade; Sara G. Milner; Axel Himmelbach; Martin Mascher; Paolo Bagnaresi; Primetta Faccioli; Paolo Cozzi; Massimiliano Lauria; Barbara Lazzari; Alessandra Stella; Andrea Manconi; Matteo Gnocchi; Marco Moscatelli; Raz Avni; Jasline Deek; Sezgi Biyiklioglu; Elisabetta Frascaroli; Simona Corneti; Silvio Salvi; Gabriella Sonnante; Francesca Desiderio; Caterina Mare; Cristina Crosatti; Erica Mica; Hakan Özkan; Benjamin Kilian; Pasquale De Vita; Daniela Marone; Reem Joukhadar; Elisabetta Mazzucotelli; Domenica Nigro; Agata Gadaleta; Shiaoman Chao; Justin D. Faris; Arthur T. O. Melo; Michael Pumphrey; Nicola Pecchioni; Luciano Milanesi; Krystalee Wiebe; Jennifer Ens; Ron P. MacLachlan; John M. Clarke; Andrew G. Sharpe; Chu Shin Koh; Kevin Y. H. Liang; Gregory J. Taylor; Ron Knox; Hikmet Budak; Anna M. Mastrangelo; Steven S. Xu; Nils Stein; Iago Hale; Assaf Distelfeld; Matthew J. Hayden; Roberto Tuberosa; Sean Walkowiak; Klaus F. X. Mayer; Aldo Ceriotti; Curtis J. Pozniak; Luigi Cattivelli. Durum wheat genome highlights past domestication signatures and future improvement targets. Nature Genetics 2019, 51, 885 -895.
AMA StyleMarco Maccaferri, Neil S. Harris, Sven O. Twardziok, Raj K. Pasam, Heidrun Gundlach, Manuel Spannagl, Danara Ormanbekova, Thomas Lux, Verena M. Prade, Sara G. Milner, Axel Himmelbach, Martin Mascher, Paolo Bagnaresi, Primetta Faccioli, Paolo Cozzi, Massimiliano Lauria, Barbara Lazzari, Alessandra Stella, Andrea Manconi, Matteo Gnocchi, Marco Moscatelli, Raz Avni, Jasline Deek, Sezgi Biyiklioglu, Elisabetta Frascaroli, Simona Corneti, Silvio Salvi, Gabriella Sonnante, Francesca Desiderio, Caterina Mare, Cristina Crosatti, Erica Mica, Hakan Özkan, Benjamin Kilian, Pasquale De Vita, Daniela Marone, Reem Joukhadar, Elisabetta Mazzucotelli, Domenica Nigro, Agata Gadaleta, Shiaoman Chao, Justin D. Faris, Arthur T. O. Melo, Michael Pumphrey, Nicola Pecchioni, Luciano Milanesi, Krystalee Wiebe, Jennifer Ens, Ron P. MacLachlan, John M. Clarke, Andrew G. Sharpe, Chu Shin Koh, Kevin Y. H. Liang, Gregory J. Taylor, Ron Knox, Hikmet Budak, Anna M. Mastrangelo, Steven S. Xu, Nils Stein, Iago Hale, Assaf Distelfeld, Matthew J. Hayden, Roberto Tuberosa, Sean Walkowiak, Klaus F. X. Mayer, Aldo Ceriotti, Curtis J. Pozniak, Luigi Cattivelli. Durum wheat genome highlights past domestication signatures and future improvement targets. Nature Genetics. 2019; 51 (5):885-895.
Chicago/Turabian StyleMarco Maccaferri; Neil S. Harris; Sven O. Twardziok; Raj K. Pasam; Heidrun Gundlach; Manuel Spannagl; Danara Ormanbekova; Thomas Lux; Verena M. Prade; Sara G. Milner; Axel Himmelbach; Martin Mascher; Paolo Bagnaresi; Primetta Faccioli; Paolo Cozzi; Massimiliano Lauria; Barbara Lazzari; Alessandra Stella; Andrea Manconi; Matteo Gnocchi; Marco Moscatelli; Raz Avni; Jasline Deek; Sezgi Biyiklioglu; Elisabetta Frascaroli; Simona Corneti; Silvio Salvi; Gabriella Sonnante; Francesca Desiderio; Caterina Mare; Cristina Crosatti; Erica Mica; Hakan Özkan; Benjamin Kilian; Pasquale De Vita; Daniela Marone; Reem Joukhadar; Elisabetta Mazzucotelli; Domenica Nigro; Agata Gadaleta; Shiaoman Chao; Justin D. Faris; Arthur T. O. Melo; Michael Pumphrey; Nicola Pecchioni; Luciano Milanesi; Krystalee Wiebe; Jennifer Ens; Ron P. MacLachlan; John M. Clarke; Andrew G. Sharpe; Chu Shin Koh; Kevin Y. H. Liang; Gregory J. Taylor; Ron Knox; Hikmet Budak; Anna M. Mastrangelo; Steven S. Xu; Nils Stein; Iago Hale; Assaf Distelfeld; Matthew J. Hayden; Roberto Tuberosa; Sean Walkowiak; Klaus F. X. Mayer; Aldo Ceriotti; Curtis J. Pozniak; Luigi Cattivelli. 2019. "Durum wheat genome highlights past domestication signatures and future improvement targets." Nature Genetics 51, no. 5: 885-895.
Breeding new crop cultivars with efficient root systems carries great potential to enhance resource use efficiency and plant adaptation to unstable climates. Here, we evaluated the natural variation of root system architectural traits in a diverse spring barley association panel and conducted genome-wide association mapping to identify genomic regions associated with root traits. For six studied traits, root system depth, root spreading angle, seminal root number, total seminal root length, and average seminal root length 1.9- to 4.2-fold variations were recorded. Using a mixed linear model, 55 QTLs were identified cumulatively explaining between 12.1% of the phenotypic variance for seminal root number to 48.1% of the variance for root system depth. Three major QTLs controlling root system depth, root spreading angle and total seminal root length were found on Chr 2H (56.52 cM), Chr 3H (67.92 cM), and Chr 2H (76.20 cM) and explained 12.4%, 18.4%, and 22.2% of the phenotypic variation, respectively. Meta-analysis and allele combination analysis indicated that root system depth and root spreading angle are valuable candidate traits for improving grain yield by pyramiding of favorable alleles.
Zhongtao Jia; Ying Liu; Benjamin D. Gruber; Kerstin Neumann; Benjamin Kilian; Andreas Graner; Nicolaus Von Wirén. Genetic Dissection of Root System Architectural Traits in Spring Barley. Frontiers in Plant Science 2019, 10, 400 .
AMA StyleZhongtao Jia, Ying Liu, Benjamin D. Gruber, Kerstin Neumann, Benjamin Kilian, Andreas Graner, Nicolaus Von Wirén. Genetic Dissection of Root System Architectural Traits in Spring Barley. Frontiers in Plant Science. 2019; 10 ():400.
Chicago/Turabian StyleZhongtao Jia; Ying Liu; Benjamin D. Gruber; Kerstin Neumann; Benjamin Kilian; Andreas Graner; Nicolaus Von Wirén. 2019. "Genetic Dissection of Root System Architectural Traits in Spring Barley." Frontiers in Plant Science 10, no. : 400.
Seeds of domesticated barley are grouped into two distinct types, which differ in morphology. Caryopses covered by adaxial (palea) and abaxial (lemma) hulls that tightly adhere to the pericarp at maturity give rise to hulled seeds whereas caryopses without adhering hulls give rise to naked seeds. The naked caryopsis character is an essential trait regarding the end use of barley. To uncover the genetic basis of the trait, a genome-wide association study (GWAS) has been performed in a panel comprising 222 2-rowed and 303 6-rowed spring barley landrace accessions. In addition to the well-described Nud locus on chromosome 7H, three novel loci showed strong associations with the trait: the first locus on 2H was specifically detected in 6-rowed accessions, the second locus on 3H was found in 2-rowed accessions from Eurasia and the third locus on 6H was revealed in 6-rowed accessions from Ethiopia. PCR analysis of naked accessions also confirmed the absence of a 17 kb region harboring the Nud gene on chromosome 7H for all but one naked accession. The latter was characterized by a slightly variant phenotype of the caryopsis. Our findings provide evidence of the pervasiveness of the 17 kb deletion in spring barley from different geographic regions and at the same time reveal genomic footprints of selection in naked barley, which follow both geographic and morphological patterns.
Celestine Wabila; Kerstin Neumann; Benjamin Kilian; Volodymyr Radchuk; Andreas Graner. A tiered approach to genome-wide association analysis for the adherence of hulls to the caryopsis of barley seeds reveals footprints of selection. BMC Plant Biology 2019, 19, 95 .
AMA StyleCelestine Wabila, Kerstin Neumann, Benjamin Kilian, Volodymyr Radchuk, Andreas Graner. A tiered approach to genome-wide association analysis for the adherence of hulls to the caryopsis of barley seeds reveals footprints of selection. BMC Plant Biology. 2019; 19 (1):95.
Chicago/Turabian StyleCelestine Wabila; Kerstin Neumann; Benjamin Kilian; Volodymyr Radchuk; Andreas Graner. 2019. "A tiered approach to genome-wide association analysis for the adherence of hulls to the caryopsis of barley seeds reveals footprints of selection." BMC Plant Biology 19, no. 1: 95.
Wild and cultivated barley are characterized by a high level of genetic diversity and a pronounced geographic population structure. Numerous studies using a diversity of markers showed that the centre of diversity of both wild and cultivated barley is in the Western part of the Fertile Crescent where the species was presumably domesticated. Comparisons of geographic diversity patterns suggested additional centres of domestication, of which the Eastern part of the Fertile Crescent (Iran or Himalaya), are most strongly supported. In wild barley, the geographic distribution of genetic and phenotypic diversity largely follows a neutral isolation by distance pattern, but common-garden experiments and environmental association studies indicate that local adaptation by natural selection also had a significant influence on these patterns but, so far no strong candidate genes for local adaptation were identified. Cultivated barley landraces and elite material have a significantly reduced level of genetic diversity compared to wild barley which also shows significant geographic differentiation and evidence for local adaptation. Several major domestication genes have already been cloned and patterns of diversity largely confirm the hypotheses that these genes were exposed to strong domestication-related selection that caused a reduction of diversity in these genes. Ex situ genebank collections of wild and domesticated barley were used to define core collections that have been phenotyped and genotyped to facilitate allele mining and introgression into elite varieties. The future utilization of barley genetic diversity will be facilitated by a good reference genome. The rapid progress of sequencing technologies and modern breeding methods like genomic selection and genome editing will contribute to an efficient utilization of barley genetic diversity.
Karl Schmid; Benjamin Kilian; Joanne Russell. Barley Domestication, Adaptation and Population Genomics. Compendium of Plant Genomes 2018, 317 -336.
AMA StyleKarl Schmid, Benjamin Kilian, Joanne Russell. Barley Domestication, Adaptation and Population Genomics. Compendium of Plant Genomes. 2018; ():317-336.
Chicago/Turabian StyleKarl Schmid; Benjamin Kilian; Joanne Russell. 2018. "Barley Domestication, Adaptation and Population Genomics." Compendium of Plant Genomes , no. : 317-336.
Genebanks are a rich source of genetic variation. Most of this variation is absent in breeding programs but may be useful for further crop plant improvement. However, the lack of phenotypic information forms a major obstacle for the educated choice of genebank accessions for research and breeding. A promising approach to fill this information gap is to exploit historical information gathered routinely during seed regeneration cycles. Still, this data is characterized by a high non-orthogonality hampering their analysis. By examining historical data records for flowering time, plant height, and thousand grain weight collected during 70 years of regeneration of 6,207 winter wheat (Triticum aestivum L.) accessions at the German Federal ex situ Genebank, we aimed to elaborate a strategy to analyze and validate non-orthogonal historical data in order to charge genebank information platforms with high quality ready-to-use phenotypic information. First, a three-step quality control assessment considering the plausibility of trait values and a standard as well as a weather parameter index based outlier detection was implemented, resulting in heritability estimates above 0.90 for all three traits. Then, the data was analyzed by estimating best linear unbiased estimations (BLUEs) applying a linear mixed-model approach. An in silico resampling study mimicking different missing data patterns revealed that accessions should be regenerated in a random fashion and not blocked by origin or acquisition date in order to minimize estimation biases in historical data sets. Validation data was obtained from multi-environmental orthogonal field trials considering a random subsample of 3,083 accessions. Correlations above 0.84 between BLUEs estimated for historical data and validation trials outperformed previous approaches and confirmed the robustness of our strategy as well as the high quality of the historical data. The results indicate that the IPK winter wheat collection reveals an extraordinary high phenotypic diversity compared to other collections. The quality checked ready-to-use phenotypic information resulting from this study is the first brick to extend traditional, conservation driven genebanks into bio-digital resource centers.
Norman Philipp; Stephan Weise; Markus Oppermann; Andreas Börner; Andreas Graner; Jens Keilwagen; Benjamin Kilian; Yusheng Zhao; Jochen C. Reif; Albert W. Schulthess. Leveraging the Use of Historical Data Gathered During Seed Regeneration of an ex Situ Genebank Collection of Wheat. Frontiers in Plant Science 2018, 9, 609 .
AMA StyleNorman Philipp, Stephan Weise, Markus Oppermann, Andreas Börner, Andreas Graner, Jens Keilwagen, Benjamin Kilian, Yusheng Zhao, Jochen C. Reif, Albert W. Schulthess. Leveraging the Use of Historical Data Gathered During Seed Regeneration of an ex Situ Genebank Collection of Wheat. Frontiers in Plant Science. 2018; 9 ():609.
Chicago/Turabian StyleNorman Philipp; Stephan Weise; Markus Oppermann; Andreas Börner; Andreas Graner; Jens Keilwagen; Benjamin Kilian; Yusheng Zhao; Jochen C. Reif; Albert W. Schulthess. 2018. "Leveraging the Use of Historical Data Gathered During Seed Regeneration of an ex Situ Genebank Collection of Wheat." Frontiers in Plant Science 9, no. : 609.
A collection of 379 Hordeum vulgare cultivars, comprising all combinations of spring and winter growth habits with two and six row ear type, was screened by genome wide association analysis to discover alleles controlling traits related to grain yield. Genotypes were obtained at 6,810 segregating gene-based single nucleotide polymorphism (SNP) loci and corresponding field trial data were obtained for eight traits related to grain yield at four European sites in three countries over two growth years. The combined data were analyzed and statistically significant associations between the traits and regions of the barley genomes were obtained. Combining this information with the high resolution gene map for barley allowed the identification of candidate genes underlying all scored traits and superposition of this information with the known genomics of grain trait genes in rice resulted in the assignation of 13 putative barley genes controlling grain traits in European cultivated barley. Several of these genes are associated with grain traits in both winter and spring barley. Copyright © 2018. . Copyright © 2018 Crop Science Society of America
Xin Xu; Rajiv Sharma; Alessandro Tondelli; Joanne Russell; Jordi Comadran; Florian Schnaithmann; Klaus Pillen; Benjamin Kilian; Luigi Cattivelli; William T. B. Thomas; Andrew J. Flavell. Genome‐Wide Association Analysis of Grain Yield‐Associated Traits in a Pan‐European Barley Cultivar Collection. The Plant Genome 2018, 11, 170073 .
AMA StyleXin Xu, Rajiv Sharma, Alessandro Tondelli, Joanne Russell, Jordi Comadran, Florian Schnaithmann, Klaus Pillen, Benjamin Kilian, Luigi Cattivelli, William T. B. Thomas, Andrew J. Flavell. Genome‐Wide Association Analysis of Grain Yield‐Associated Traits in a Pan‐European Barley Cultivar Collection. The Plant Genome. 2018; 11 (1):170073.
Chicago/Turabian StyleXin Xu; Rajiv Sharma; Alessandro Tondelli; Joanne Russell; Jordi Comadran; Florian Schnaithmann; Klaus Pillen; Benjamin Kilian; Luigi Cattivelli; William T. B. Thomas; Andrew J. Flavell. 2018. "Genome‐Wide Association Analysis of Grain Yield‐Associated Traits in a Pan‐European Barley Cultivar Collection." The Plant Genome 11, no. 1: 170073.