The work in different project positions provided me a deep insight in the technical aspects of OFM and other perfusion techniques, project managing and group managing. These experiences provided me with the expertise, leadership and motivation necessary to successfully carry out the proposed work. I have a broad background in biochemical engineering, inter-disciplinary medical research, in-vivo sampling techniques. I have specific training and expertise in open flow microperfusion, preclinical surgery, quality management systems and project management for this application. During my time as a PhD student, I developed a cerebral application of the open flow microperfusion system which has become a respected research tool for basic neuroscience and pharmaceutical industry. I successfully participated and led various big research projects grouped around the open flow microperfusion (e.g. EU projects BioNanoPharmaceutics, and national financed projects BMVIT projects (ProMes) and FFG (IDT4Brain) - all with a budget above 1 million Euro.