Lactobacillus plantarum TWK10 (LP10) is a probiotic known to improve endurance exercise performance. Here, we analyze the proteomics and metagenomic changes in a LP10 supplemented mouse model. Male ICR mice were divided into two groups (n = 8) to receive by oral gavage either vehicle or of LP10 for 6 weeks. Proteins changes by LP10 treatment were subjected to the Ingenuity Pathway Analysis (IPA) to provide corroborative evidence for differential regulation of molecular and cellular functions affecting metabolic processes. Fecal samples were obtained from each mouse, and the microbial community profile analyzed by pyrosequencing of the 16S rRNA genes. Of the 880 identified proteins, 25 proteins were significantly downregulated and 44 proteins were significantly upregulated in the LP10 treated compared to vehicle group. LP10 supplementation shift in the gut microbiota to butyrate‐producing members and provided from lipid oxidation since peroxisomal fatty acid oxidation in liver.

The risk of non-union and prolonged periods of protected weight-bearing still remain unsolved issues after distal femur osteotomy (DFO). To improve the stability, we developed the double chevron-cut technique, which is a modified medial closing-wedge DFO guided by a patient-specific instrument. The purpose of this study was to investigate the feasibility and outcome of this operative approach. Twenty-five knees in twenty-three consecutive patients with genu valgum and lateral compartment osteoarthritis that received double chevron-cut DFO were included. The target of correction was 50% on the weight-bearing line (WBL) ratio. Patient-reported outcomes included the Oxford Knee Score (OKS) and the 2011 Knee Society Score (KSS). The mean of the WBL ratio was corrected from 78.7% ± 12.0% to 48.7% ± 2.9% postoperatively. The mean time to full weight bearing was 3.7 ± 1.4 weeks. Union of the osteotomy was achieved at 11.3 ± 2.8 weeks. At a mean follow-up of 17 months, the OKS improved from a mean of 27.6 ± 11.7 to 39.1 ± 7.5 (p = 0.03), and the KSS from a mean of 92.1 ± 13.0 to 143.9 ± 10.2 (p < 0.001). Three patients developed complications, including one case of peri-implant fracture, one of loss of fixation, and one of non-union. The double chevron-cut DFO followed by immediate weight-bearing as tolerated is effective in treating genu valgum deformity and associated lateral compartment osteoarthritis.

Gouty arthritis is the most common form of inflammatory arthritis and flares frequently after surgeries. Such flares impede early patient mobilization and lengthen hospital stays; however, little has been reported on gout flares after spinal procedures. This study reviewed a database of 6439 adult patients who underwent thoracolumbar spine surgery between January 2009 and June 2021, and 128 patients who had a history of gouty arthritis were included. Baseline characteristics and operative details were compared between the flare-up and no-flare groups. Multivariate logistic regression was used to analyze predictors and construct a predictive model of postoperative flares. This model was validated using a receiver operating characteristic (ROC) curve analysis. Fifty-six patients (43.8%) had postsurgical gout flares. Multivariate analysis identified gout medication use (odds ratio [OR], 0.32; 95% confidence interval [CI], 0.14–0.75; p = 0.009), smoking (OR, 3.23; 95% CI, 1.34–7.80; p = 0.009), preoperative hemoglobin level (OR, 0.68; 95% CI, 0.53–0.87; p = 0.002), and hemoglobin drop (OR, 1.93; 95% CI, 1.25–2.96; p = 0.003) as predictors for postsurgical flare. The area under the ROC curve was 0.801 (95% CI, 0.717–0.877; p < 0.001). The optimal cut-off point of probability greater than 0.453 predicted gout flare with a sensitivity of 76.8% and specificity of 73.2%. The prediction model may help identify patients at an increased risk of gout flare.

BackgroundDistal femur osteotomy (DFO) has been gaining popularity in treating subjects with genu valgum and the associated lateral compartment osteoarthritis. However, the risk of non-union and a period of protective weight bearing still remain unsolved even with the advent of locking plates. To improve the inherent stability of medial close-wedge DFO, we create bone cut in chevron shape with the guidance of a patient-specific instrument (PSI). The patients were allowed immediate weight-bearing as tolerated. The objective of the study was to assess the results of this technique.MethodsTwenty-five knees in twenty-three consecutive patients with genu valgum and lateral compartment osteoarthritis received chevron-cut medial close-wedge DFO. The alignment parameters of the knee, including the weight-bearing line (WBL) ratio, hip-knee-ankle angle (HKA), and mechanical lateral distal femoral angle (mLDFA) were assessed. We defined outliers as those with a difference of more than 10% from the targeted 50% WBL ratio. Patient-reported evaluation included the Oxford Knee Score (OKS), Knee Society Score (KSS), and a visual analog scale (VAS) pain scale.ResultsThe WBL ratio, HKA, and mLDFA were corrected from a mean of 78.7% ± 12.0%, 9.3° ± 2.8° valgus, and 83.6° ± 1.9° to 48.7% ± 2.9%, 0.5° ± 1.1° varus, and 91.4° ± 3.5° (respectively) postoperatively. A mean operative time of 58.8 ± 18.3 minutes, and 6.2 ± 1.3 intraoperative radiographs were taken. A mean Hb drop of 1.4 g/dl was found, while no patient required transfusion. Only one knee (4%) postoperatively fell in the defined range of correction outliers. Consolidation of the osteotomy was achieved at 11.3 ± 2.8 weeks. The OKS, KSS, and VAS pain scale were significantly improved compared with the pre-operative data. Complications developed in three patients, including one periprosthetic fracture, one loss of fixation, and a case of non-union.ConclusionThe PSI-guided chevron-cut close-wedge DFO, followed by immediate weight-bearing as tolerated is accurate, safe, and effective in the correction of genu valgum deformity and the associated lateral compartment osteoarthritis.Level of evidence IV