The primary objective of Tissue engineering is a regeneration or replacement of tissues or organs damaged by disease, injury, or congenital anomalies. At present, Tissue engineering repairs damaged tissues and organs with artificial supporting structures called scaffolds. These are used for attachment and subsequent growth of appropriate cells. During the cell growth gradual biodegradation of the scaffold occurs and the final product is a new tissue with the desired shape and properties. In recent years, research workplaces are focused on developing scaffold by bio-fabrication techniques to achieve fast, precise and cheap automatic manufacturing of these structures. Most promising techniques seem to be Rapid prototyping due to its high level of precision and controlling. However, this technique is still to solve various issues before it is easily used for scaffold fabrication. In this article we tested printing of clinically applicable scaffolds with use of commercially available devices and materials. Research presented in this article is in general focused on “scaffolding” on a field of bone tissue replacement. Commercially available 3D printer and Polylactic acid were used to create originally designed and possibly suitable scaffold structures for bone tissue engineering. We tested printing of scaffolds with different geometrical structures. Based on the osteosarcoma cells proliferation experiment and mechanical testing of designed scaffold samples, it will be stated that it is likely not necessary to keep the recommended porosity of the scaffold for bone tissue replacement at about 90%, and it will also be clarified why this fact eliminates mechanical properties issue. Moreover, it is demonstrated that the size of an individual pore could be double the size of the recommended range between 0.2–0.35 mm without affecting the cell proliferation. Rapid prototyping technique based on Fused deposition modelling was used for the fabrication of designed scaffold structures. All the experiments were performed in order to show how to possibly solve certain limitations and issues that are currently reported by research workplaces on the field of scaffold bio-fabrication. These results should provide new valuable knowledge for further research.

The aim of the research is to analyze the possibility of the development and realization of a common laser triangulation sensor arrangement-based probe for the measurement of slots and bore sides with the help of a mirror attachment. The analysis shows the feasibility and limits of the solution with respect to the maximum measurement depth and surface distance measurement working range. We propose two possible solutions: one for maximizing the ratio of the measurement depth to the measured bore size and the second for maximizing the total depth, intended for the measurement of slots and large bore sizes. We analyzed measurement error sources. We found that the errors related to the reflection mirror misalignment can be fully compensated. We proved the validity of the proposed solution with the realization of a commercial laser triangulation sensor-based probe and demonstrated a slot side and a bore side surface distance scanning measurement. The probe working range was assessed with regard to the obscuration effect of optical beams.

We propose a miniaturised light stage for measuring the bidirectional reflectance distribution function (BRDF) and the bidirectional texture function (BTF) of surfaces on site in real world application scenarios. The main principle of our lightweight BTF acquisition gantry is a compact hemispherical skeleton with cameras along the meridian and with light emitting diode (LED) modules shining light onto a sample surface. The proposed device is portable and achieves a high speed of measurement while maintaining high degree of accuracy. While the positions of the LEDs are fixed on the hemisphere, the cameras allow us to cover the range of the zenith angle from 0 ${}^{\circ }$ to 75 ${}^{\circ }$ and by rotating the cameras along the axis of the hemisphere we can cover all possible camera directions. This allows us to take measurements with almost the same quality as existing stationary BTF gantries. Two degrees of freedom can be set arbitrarily for measurements and the other two degrees of freedom are fixed, which provides a tradeoff between accuracy of measurements and practical applicability. Assuming that a measured sample is locally flat and spatially accessible, we can set the correct perpendicular direction against the measured sample by means of an auto-collimator prior to measuring. Further, we have designed and used a marker sticker method to allow for the easy rectification and alignment of acquired images during data processing. We show the results of our approach by images rendered for 36 measured material samples.

Recent developments in optoelectronics and material processing techniques make it possible to design and produce a portable and compact measurement instrument for bidirectional texture function (BTF). Parallelized optics, on-board data processing, rapid prototyping, and other nonconventional production techniques and materials were the key to building an instrument capable of in situ measurements with fast data acquisition. We designed, built, and tested a prototype of a unique portable and compact multi-camera system for BTF measurement which is capable of in situ measurement of temporally unstable samples. In this paper, we present its optomechanical design.

Optical systems are traditionally used for accurate recording and measurement of the real world’s appearance. Present techniques allow us to form a computer-based virtual world, which is used in a variety of technical fields. The crucial issue for future applications of virtual reality is the fidelity of rendered images to real-world objects. This is strongly affected by the appearance of the rendered object’s surfaces. Currently, the most applied method of describing a surface’s visual appearance of spatially nonuniform surfaces is bidirectional texture function (BTF). We have designed, optimized, built, and tested a unique portable instrument based on a rotary kaleidoscope principle for BTF acquisition in situ. To the best of our knowledge, such an instrument has never been used before to measure BTF of a surface. We enhanced a common static kaleidoscope by adding rotation, which allows us to get a larger number of images of the sample for more combinations of illumination directions and viewing directions. This results in a higher directional and spatial resolution of measured BTF data. In this paper, we focus on the optomechanical design of the rotary BTF measurement instrument and issues related to its alignment to keep the desired mechanical precision.

The task of imaging optics design starts with choosing a subset of imaging parameters such as the object distance, the image magnification, the image resolution, and the depth of field. It is clear that the imaging parameters are inter-related and their appropriate trade-off is the key problem of final optical design. We provide a novel analy-sis of the optical design considering diffractive imaging phenomena and thin lens approximation, and we derive formulas for the solution of individual parameters. We show that combinations of three independent optical design input variables determine all other optical and geometrical parameters under diffraction-limited optical imaging. We also formulate an invariant relation in the object space if the depth of field is one of the design's target parameters. (c) 2022 Optica Publishing Group