Edible insects such as the black soldier fly Hermetia illucens L. represent a potential and sustainable source of nutrients for food and feed due to their valuable nutritional composition, which can be modulated through dietary enrichment. The high content of saturated fatty acid (FA) of Hermetia illucens larvae fats can be modulated through dietary enrichment as a result of adding vegetable oils in the rearing substrate. Therefore, the present research aims to highlight the effects of a 10% addition of vegetable oils from five dietary fat sources (linseed oil, soybean oil, sunflower oil, rapeseed oil, and hempseed oil) on the growth, development, reproductive performance, and the fat and fatty acids profile of H. illucens. Oil inclusion in the larval diet improved (p < 0.05) the weight of larvae, prepupae, pupae, and imago without influencing (p > 0.05) the egg clutch weight and the number of eggs in the clutch. In addition, the larvae fatty acid profile was different (p < 0.001) according to the oil type, because the unsaturated FAs (UFA) increased from 11.23 to 48.74% of FAME, as well as according to the larvae age, because the saturated FAs decreased from 85.86 to 49.56% of FAME. Linseed oil inclusion led to the improvement of the FA profile at 10 days age of larvae, followed by hempseed and rapeseed oil. These three dietary treatments recorded the highest concentrations in UFA (29.94–48.74% of FAME), especially in polyunsaturated FA (18.91–37.22% of FAME) from the omega-3 series (3.19–15.55% of FAME) and the appropriate n–6/n–3 ratio. As a result, the degree of the lipid polyunsaturation index increased (17.76–41.44) and the value of the atherogenic (3.22–1.22) and thrombogenic (1.43–0.48) indices decreased. Based on the obtained results, it can be concluded that enriching the larval diet with these oils rich in UFA can modulate the larvae FA profile, making them suitable sources of quality fats for feed and indirectly for food.

Hermetia illucens is a sustainable and an increasingly bioeconomical source of nutrients for farm animals. It is still necessary, however, to improve our knowledge of the biological features of this species in order to maximize its use. The aims of this research were to evaluate the effect of the energy level of rearing diets on its body weight and fat-body reserves. The quantity and quality of the fats storred by the non-feeding stages of this insect and its reproductive performances were also studied. A control diet (CD - Gainesville diet) and three diets with progressively greater energy contents (kcal/kg ME) were formulated. The increase was achieved by including different amounts of maize, 40% (ED₁), 60% (ED₂) and 80% (ED₃), in these three diets. Abother diet (ED₄) consisted of fruit and vegetable waste. The results indicate that the body weight of larvae, prepupae, pupae and adult flies, as well as fat content of the larvae increased significantly (p ˂ 0.01) with increase in the energy content of the diets. There was a positive Pearson correlation between energy content of diets and body weight of adult flies. The heaviest egg clutches with the highest number of eggs/clutch (p ˂ 0.01) were laid by flies reared on the diets with the highest energy content. The quality of the fat stored by larvae did not influence the weight and number of egg laid.

The research objective was to evaluate the effect of dietary incorporation of hemp seeds in goats fed with hay or mixed shrubs–grass rangeland on the production, FA profile and health-related lipid indices, antioxidant content and total antioxidant capacity of milk, with the purpose to increase the content of beneficial ingredients in milk and to improve its functional activity. Forty indigenous Carpathian goats were allocated into two groups according to the type of basic forage in their diet: hay (H) or mixed shrubs–grass rangeland (SG); each of them was further divided into two subgroups according to the presence of Hs in the concentrate mixture (250 g/kg) or not. Milk production was determined, and milk samples were collected and analyzed for fat, protein, lactose, and cholesterol content, as well as FA profile, lipophilic antioxidant content (α-tocopherol and retinol), and milk TAC. SG goats gave less milk (p < 0.01) but with higher milk fat (p < 0.001) and lower cholesterol content (p < 0.01) than H goats, while milk protein and lactose contents were not affected. Supplementing the diet with Hs caused a significant increase in milk production (p < 0.05) and milk fat content (p < 0.001) and a decrease in cholesterol content (p < 0.05). Grazing compared to indoor feeding but also supplementing the diet with Hs had the effect of decreasing the proportion of SFAs and increasing the concentrations of polyunsaturated FA (PUFA) in goat milk fat (p < 0.01). Fats in the milk of SG goats compared to H, but also in those supplemented with Hs, showed significantly higher proportions of vaccenic acid (VA), rumenic acid (CLA c-9,t-11) and omega-3 FA (α-linolenic acid (ALA), eicosapentaenoic acid (EPA), and docosapentaenoic acid (DPA)) which are considered healthy for consumers. The feeding system based on SG and the diet supplementation with Hs ensured the best nutritional and functional quality of milk, confirmed by the FA profile, antioxidant content, and by the value of health-related lipid indices (n-6/n-3 FA ratio and hypo-/hypercholesterolemia, atherogenic index (AI), thrombogenic index (TI), and health promotion index (HPI)). The results of our work will be useful for the development of optimal nutritional strategies that improve the FA profile and the antioxidants content in goat milk, with beneficial effects on human health.

The industrial rearing of Hermetia illucens offers sustainable solutions to the acute challenges of modern society associated with the accumulation of increasing amounts of organic waste, the substantial reduction of natural ocean fish stocks, and the imminent food crisis. Detailed knowledge of the reproductive particularities and reproductive behavior of the species is essential for increasing the efficiency of the breeding technology. This study aimed to identify the affinity shown by females regarding the size of the oviposition slots (1, 2, 3, 4 and 5 mm), the vertical distribution of the ovipositing rate, and the influence of the substrate’s moisture on ovipositing behavior (dry matter/water: 1:0.5; 1:1; 1:1.5; 1:2). Over 90% of females oviposited in the 1-, 2- and 3-mm slots, with most of the eggs (58.57%) being oviposited in the 1-mm slots. There was a positive correlation between the size of the oviposition slots and the average weight of the clutches (r = 0.985). The vertical distribution of ovipositing followed a fluctuating trend, with a tendency to oviposit closer to the attractive substrate. The females avoided ovipositing close to substrates with low humidity (1:0.5); indeed, only 6.8% oviposited under these conditions, the differences being significant compared to substrates with higher humidity (p < 0.001). The vast majority of females (43.2%) oviposited on substrates with an average humidity of 1:1 (p < 0.001). These results provide new insights into the ovipositing behavior of H. illucens, which allow for a differentiated harvest of large-sized clutches, providing practical applications with significant impact on the economic efficiency of the species’ industrial growth technology.

Lupinus albus seeds from low-alkaloid varieties are a valuable alternative source of protein for reducing the dependence on soybean meal for the feeding of monogastric animals. Therefore, the aim of this research was to evaluate the dehulling influence of lupine seeds (L. albus, cv. Amiga) and the effect of their use in the laying quails feed on live performance and egg quality. A total of 200 laying quails (Coturnix japonica), with an age of 10 weeks, were randomly assigned to five experimental groups (five replicates/group). Each group was randomly assigned to one of five dietary treatments: the control group (C) diet was based on soybean meal, while the experimental groups received either 200 g/kg (WLS₂₀) or 250 g/kg (WLS₂₅) of whole lupine seeds in their diets, or 200 g/kg (DLS₂₀) or 250 g/kg (DLS₂₅) of dehulled lupine seeds in their diets. The results show that by dehulling the lupine seeds, the nutritional value of the seeds increased. The use of dehulled lupine seeds in the diet of laying quails did not affect the live performance (egg production, feed intake, feed conversion ratio), yolk cholesterol content, and physico-chemical quality indices of the eggs, compared with control. The presence of dehulled lupine seeds in the diet improved the nutritional quality of the yolk lipids because of the increase in the content of polyunsaturated fatty acids. Thus, the lipid quality indices of the yolk became more favorable to consumer health because of an increase in the h/H ratio (hypocholesterolemic/Hypercholesterolemic FA) and a decrease in the atherogenic index and thrombogenic index values. The higher content in carotenoids also contributed to the higher nutritional quality of the eggs obtained by lupine utilization. In conclusion, dehulling of lupine seeds had a positive influence on the nutritional quality of the seeds, the live performance of quails, and the quality of eggs.

The significant momentum received by Hermetia illucens as a worldwide species is due to its biological traits and large applicability in scientific research, environmental entomoremediation, insect meal production, and for biodiesel yield. The aim of this research is to develop a method for the preparation and precise egg counting of the H. illucens egg clutch, as well as an accurate technique for evaluating egg biometric parameters. The precise proposed method for egg preparation and counting consists in dispersing the eggs clutch under a stereo microscope and counting the eggs on a photographic capture using the Clickmaster software. Five solution types were used to disperse the egg clutches: glycerin 50%, ethanol 70%, ethanol 80%, physiological serum 0.9% and purified water. The efficiency of the estimation method for eggs number evaluation was also tested by using the estimated egg weight as a conversion factor. The biometric parameters of single eggs (length and width) were determined using the free Toupview software. The precise method of egg preparation and counting allows for the registration of the eggs number manually identified by the operator. The appropriate dispersion solutions were glycerin 50% and ethanol 70%. The method has an error of 1.4 eggs for each 500 counted eggs, thus an accuracy of over 99.4%. The eggs number estimation method is not applicable without significant errors, the accuracy being less than 32%, due to egg heterogeneity in the clutch. Biometric parameters (length and width) are positively correlated with egg weight (r = 0.759) and with the number of eggs in the clutch (r = 0.645). In conclusion, the results clearly suggest the method of egg preparation and precise counting for an accurate evaluation of quality parameters of the H. illucens clutches, as well as the technique for evaluating egg biometric parameters.