Following the increase in the employment of women in conflicts around the world, the federal government of Brazil enacted a law which determines the participation of women in the military. The aim of this study was to analyze the effect of six months of physical training (PT) on the physical fitness of young Brazilian Army cadets to carry out the physical assessments provided in military training. Sixty-eight members of the (19.4 ± 1.0 years) military from the Brazilian Army (BA), with BMI of (23.61 ± 2.17/21.81 ± 2.26) respectively and divided in two groups (men/women) participated in the study. PT was conducted by Manual EB20-MC10.350. Anthropometric measurements and assessment of body composition by dual X-ray absorptiometry were performed. The Student’s t test, percentage evolution equation, and Levene test were used. Results showedasignificant increase in anthropometric variables and cardiorespiratory fitness in both groups. Bone health variables and visceral fat presented a significant increase in the malegroup. In terms of muscle fitness handgrip and isometric strength there was no significant variation between the groups and push-up and pull-up there was significant variation between the groups. Percentage evolution was greater in female group. The conclusion shows PT was able to cause beneficial changes, promoting positive improvement in bone health, especially in women. Also, PT was shown to enhance cardiorespiratory capacity, and muscle fitness of the upper limbs in all participants.