Background: Anopheles mosquitoes are the vectors of malaria, one of the most important infectious diseases in the tropics. More than 500 Anopheles species have been described worldwide, and more than 30 are considered a public health problem. In Honduras, information on the distribution of Anopheles spp. and its genetic diversity is scarce. This study aimed to describe the distribution and genetic diversity of Anopheles mosquitoes in Honduras. Methods: Mosquitoes were captured in 8 locations in 5 malaria endemic departments during 2019. Two collection methods were used. Adult anophelines were captured outdoors using CDC light traps and by aspiration of mosquitoes at rest. Morphological identification was performed using taxonomic keys. Genetic analyses included the sequencing of a partial region of the cytochrome c oxidase 1 gene (cox1) and the ribosomal internal transcribed spacer 2 (ITS2). Results: A total of 1320 anophelines were collected and identified through morphological keys. Seven Anopheles species were identified. Anopheles albimanus was the most widespread and abundant species (74.02%). To confirm the morphological identification of the specimens, 175 and 122 sequences were obtained for cox1 and ITS2, respectively. Both markers confirmed the morphological identification. cox1 showed a greater nucleotide diversity than ITS2 in all species. High genetic diversity was observed within the populations of An. albimanus while An. darlingi proved to be a highly homogeneous population. Phylogenetic analyses revealed clustering patterns in An. darlingi and An. neivai in relation to specimens from South America. New sequences for An. crucians, An. vestitipennis and An. neivai are reported in this study. Conclusions: Here we report the distribution and genetic diversity of Anopheles species in endemic areas of malaria transmission in Honduras. According to our results, both taxonomic and molecular approaches are useful tools in the identification of anopheline mosquitoes. However, both molecular markers differ in their ability to detect intraspecific genetic diversity. These results provide supporting data for a better understanding of the distribution of malaria vectors in Honduras.

Malaria remains a life-threatening disease in many tropical countries. Honduras has successfully reduced malaria transmission as different control methods have been applied, focusing mainly on indoor mosquitoes. The selective pressure exerted by the use of insecticides inside the households could modify the feeding behavior of the mosquitoes, forcing them to search for available animal hosts outside the houses. These animal hosts in the peridomicile could consequently become an important factor in maintaining vector populations in endemic areas. Herein, we investigated the blood meal sources and Plasmodium spp. infection on anophelines collected outdoors in endemic areas of Honduras. Individual PCR reactions with species-specific primers were used to detect five feeding sources on 181 visibly engorged mosquitoes. In addition, a subset of these mosquitoes was chosen for pathogen analysis by a nested PCR approach. Most mosquitoes fed on multiple hosts (2 to 4), and 24.9% of mosquitoes had fed on a single host, animal or human. Chicken and bovine were the most frequent blood meal sources (29.5% and 27.5%, respectively). The average human blood index (HBI) was 22.1%. None of the mosquitoes were found to be infected with Plasmodium spp. Our results show the opportunistic and zoophilic behavior of Anopheles mosquitoes in Honduras.

Gracias al acceso al genoma completo de Coffea arabica y el Desarrollo de multiples herramientas de bioinformartica que permite la búsqueda de genes de resistencia de plantas (R-genes), ha sido posible implementar estas estrategias en programas de mejora genética. En las plantas, los R-genes codifican proteínas involucradas en mecanismos de defensa contra patógenos. Los genes con dominios tipo Nucleotide-Binding-Site Leucine-Rich-Repeat (NBS-LRR) forman la familia de R-genes de plantas más grande. El objetivo de este estudio fue identificar genes de proteínas NBS-LRR en el genoma de C. arabica utilizando un enfoque bioinformático. Identificamos motivos conservados de R-genes de C. arabica relacionados con genes similares encontrados en Coffea canephora y Coffea eugenoides, dos especies evolutivas relacionadas con C. arabica. Los resultados de estos análisis revelaron proteínas con origen evolutivo provenientes de dicotiledóneo ancestrales, así como proteínas de resistencia específicas del género Coffea. Además, todas las secuencias de los R-genes de C. arabica mostraron una gran similitud con proteína CNL de Arabidopsis thaliana. Finalmente, la presencia de motivos altamente conservados, la distribución cromosómica y las relaciones filogenéticas de los R-genes de C. arabica muestran procesos de coevolución con patógenos adaptados, demostrando de esta manera la importancia del estudio de estos genes en la inmunidad del café. Palabras clave: Café, NBS-LRR, Proteínas de Resistencia, Bioinformática.

Biotechnology provides sustainable solutions for soil and ecological deterioration resulting from human activities. This study investigated the biotechnological potential of strains isolated from environmental samples collected in protected areas and mines of Honduras, focusing on their application for producing biofertilizers and bioremediation. 63 samples were collected from: soil, soil covers, mine waters, and hot springs. From these, 118 bacterial strains were isolated and characterized using microbiological and molecular techniques. The genera Pseudomonas, Bacillus, Azospirillum, and Azotobacter were associated with some of the isolated strains and stood out for their ability to solubilize potassium, fix nitrogen and produce bioactive compounds. Isolates related to bacteria of the genera Bacillus and Pseudomonas demonstrated potential use in biotechnology. Furthermore, some strains exhibited significant genetic diversity and biological similarity to bacterial genera and species utilized in pollution remediation and soil fertility improvement. A small group shows atypical morphologies, including pleomorphic, filamentous structures, features with vacuole-like traits, pigment production, iridescence, and metallic sheen. These results highlight the microbial biodiversity present in soil covers of protected Honduran ecosystems and their importance as reservoirs of microorganisms with biotechnological applications. This study emphasizes the importance of preserving these natural regions, not only for biodiversity preservation but also for utilizing their potential in formulating sustainable solutions to environmental and agricultural challenges.

Arabidopsis and other model plants can help improve crops by providing a reference framework for assembling omics data from plants. Arabidopsis is extensively studied and has a well-curated and annotated genome, making it an excellent reference organism for comparative analysis. When there is no reference genome of the organism being investigated, using the genome of Arabidopsis may be an option thanks to the extensive knowledge we have about it. Crop improvement can help to increase crop yields, improve crop quality, and enhance resistance to pests, diseases, and environmental stresses. It can also help to reduce the use of pesticides and fertilizers, which can have negative impacts on the environment and human health. Therefore, crop improvement is essential to ensure food security and sustainability in the face of global challenges such as climate change, land degradation, and water scarcity. The assembly process of genomic, transcriptomic, and metabolomic data from plants using carefully selected information on Arabidopsis can lead to a deeper understanding of organisms and their potential for genetic improvement for crops. However, the successful integration of data requires expertise and experience to design more effective and precise crop improvement strategies.