Introduction: This review gives an overview of interesting properties of nanoparticles finding potential applications in nanomedicines and their considerations that need to be made such as toxicity while developing a nanomedicine by providing an understanding of a relationship between nanocarrier, targeting moieties and drugs with optical and magnetic properties. Here, we correlate the interesting properties of nanomaterials to their applications in living cells/body simultaneously promises, prospects and toxicity challenges of nanomedicines have also been discussed in detail. Exemplifying the usage of gold nanoparticles and its derivatives such as hetero and homo hybrid nanostructures that allow their use as contrast agents, therapeutic entities and supports to attach functional molecules and targeting ligand along with molecular framework structures. Here, we present the future prospects for potential applications in nanomedicines. These nanomaterials have been used for varieties of biomedical applications such as targeted drug delivery, photothermal cancer therapies, MRI, optical imaging, etc. in vitro and in vivo. Conclusion: In summary, this review provides innumerable aspects in the emerging field of nanomedicine and possible nanotoxicity.

Extraction of RNA from Dioscorea is difficult because of rich mucilage and secondary metabolite content. High-quality RNA is required for RT-PCR and transcriptome analysis. Different protocols and common extraction kits were used for RNA extraction in Dioscorea, but the results were not satisfactory. A CTAB based protocol with lithium chloride precipitation yielded good quality RNA. The RNA isolated using this protocol was successfully used for RT-PCR and transcriptome sequencing experiments. Mucilage content varies at different developmental stages of Dioscorea and the present protocol was effective in isolating RNA from such samples. Although the protocol was originally modified for tuber tissues, it can be used also for extraction of RNA from rhizome, root, shoot and leaf.

Auxin levels are tightly regulated within the plant cell, and its storage in the isolated cavity of proteins is a measure adopted by cells to maintain the availability of auxin. We report the first crystal structure of Wrightia tinctoria 11S globulin (WTG) in complex with Indole-3-acetic acid (IAA), an auxin, at 1.7 Å resolution. WTG hexamers assemble as a result of the stacking interaction between the hydrophobic surfaces of two trimers, leaving space for the binding of charged ligands. The bound auxin is stabilized by non-covalent interactions, contributed by four chains in each cavity. The presence of bound ligand was confirmed by matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) and high-resolution mass spectrometry (HRMS). Here, we hypothesize that the cleavage of globulins by endopeptidases leads to the movement of the hydrophilic loop region from the surface to the periphery, leaving space for the binding of auxin, and promotes hexamer formation. As the process of germination proceeds, there is a change in the pH, which induces the dissociation of the hexamer and the release of auxin. The compact hexameric assembly ensures the long-term, stable storage of the hormone. This suggests a role for globulin as a novel player in auxin homeostasis.