Journal of Medical Internet Research - International Scientific Journal for Medical Research, Information and Communication on the Internet #Preprint #PeerReviewMe: Warning: This is a unreviewed preprint. Readers are warned that the document has not been peer-reviewed by expert/patient reviewers or an academic editor, may contain misleading claims, and is likely to undergo changes before final publication, if accepted, or may have been rejected/withdrawn. Readers with interest and expertise are encouraged to sign up as peer-reviewer, if the paper is within an open peer-review period. Please cite this preprint only for review purposes or for grant applications and CVs (if you are the author). Background: EHealth technology has significantly impacted HIV prevention strategies, but reach and utilization among African American women has been scarce. Objective: To explore the feasibility, acceptability, and preference of an avatar, eHealth video to increase awareness and knowledge of HIV post and pre-exposure prophylaxis (PEP and PrEP) among a sample of African American women. Methods: A cross-sectional, descriptive, online study was conducted with 116 African American women ages 18 to 61. We explored: 1) consumer-awareness, 2) intention to use, and 3) preference for post and pre-exposure prophylaxis after watching the eHealth video. Results: Eighty-nine percent rated the video as good or higher. A higher rating of the educational video was significantly predicted by: no current use of drugs/alcohol (β = -.814, p = .004); not having unprotected sex in the last 3 months (β =-.488, p = .025); higher income (β =.149, p = .026); lower level of education (β =-.267, p = .005); and, a lower exposure to sexual assault since age 18 (β =-.313, p = .004). After watching the eHealth video, interest in both, PEP and PrEP was high, with intentions to use PEP and PrEP being 96.7% and 75.6% respectively. Ninety-three percent reported recommending PEP and 90.1% PrEP to other women. Conclusions: Utilization of an avatar-led, eHealth, online video fostered education about PEP and PrEP. We can leverage this approach to increase awareness and usage among African American women.