Yeast can be isolated from tofu wastewater and the cell wall in the form of β-glucan can act as a natural decontaminant agent. This study aimed to isolate and characterize native yeast from tofu wastewater, which can be extracted to obtain β-glucan and then identify the yeast and its β-glucan activity regarding antifungal ability against Aspergillus flavus and aflatoxin-reducing activity towards aflatoxin B1 (AFB1) and B2 (AFB2). Tofu wastewater native yeast was molecularly identified, and the growth observed based on optical density for 96 h and the pH also measured. β-glucan was extracted from native yeast cell walls with the acid-base method and then the inhibition activity towards A. flavus was tested using the well diffusion method and microscopic observation. AFB1 and AFB2 reduction were identified using HPLC LC-MS/MS. The results showed that the native yeast isolated was Pichia norvegensis with a β-glucan yield of 6.59%. Pichia norvegensis and its β-glucan showed an inhibition zone against Aspergillus flavus of 11.33 ± 4.93 and 7.33 ± 3.51 mm, respectively. Total aflatoxin-reducing activity was also shown by Pichia norvegensis of 26.85 ± 2.87%, and β-glucan of 27.30 ± 1.49%, while AFB1- and AFB2-reducing activity by Pichia norvegensis was 36.97 ± 3.07% and 27.13 ± 1.69%, and β-glucan was 27.13 ± 1.69% and 32.59 ± 4.20%, respectively.

The proliferation of commercialization in the development of urban agriculture (UA) within the Great Bandung region has precipitated the dislocation of numerous small-scale farmers, consequently engendering a reduction in the accessibility of locally cultivated sustenance. The resultant effect of this phenomenon is the exacerbation of food insecurity among low-income inhabitants who lack the financial wherewithal to procure the relatively expensive imported food items. Furthermore, the dearth of availability of unprocessed, high-quality sustenance has resulted in suboptimal dietary intake among a considerable proportion of the populace. A survey was conducted utilizing questionnaires to gather data from 137 units of respondents consisting of both subsistence and commercial urban farmers. The comparative analysis of the earnings of urban farmers was conducted vis-à-vis the minimum wages projected for the year 2023 in West Java and Bandung City. The utilization of the Food Insecurity Experience Scale (FIES) is a prevalent method for evaluating food insecurity through the analysis of self-reported food-related behaviors and experiences, both pre- and post-UA operations. A 24 h Food Recall Instrument examined nutrition adequacy in urban farmer households, which was analyzed using chi-square with descriptive presentation. Urban agriculture could help solve commercialization, food insecurity, and nutrition issues in the Great Bandung Region. Commercial urban farmers (51.09%) earned 280.46% of West Java provincial minimum wages or 136.82% of Bandung City minimum wages in 2023. The subsistence farmers’ cohort (48.91%) earned 14.64% of West Java provincial minimum earnings or 7.14% of Bandung City minimum wages in 2023. In general, the prevalence of ties to food insecurity was predominantly observed subsequent to UA practices. The study revealed a prevalence of vitamin A surplus among urban farmers, while a preponderance of inadequacy in other nutritional aspects was also observed.