Nanofiber membranes were successfully synthesized from expanded polystyrene (EPS) waste with the addition of poly(vinylpyrrolidone) (PVP) for water microfiltration using the electrospinning method. The EPS-based nanofiber membranes exhibited a smooth morphology and were uniform in size. The concentration of the EPS/PVP solution changed some of the physical parameters of the nanofiber membrane, such as viscosity, conductivity, and surface tension. Greater viscosity and surface tension increase the nanofiber membrane diameter, whereas the addition of PVP results in hydrophilicity. Additionally, increasing the pressure increased the flux value of each variation of the nanofiber membranes. Furthermore, the rejection value was 99.99% for all variations. Finally, the use of EPS waste for nanofiber membranes is also beneficial for decreasing the amount of EPS waste in the environment and is an alternative to the current membranes available in the market for water filtration applications.

Docking analysis of propolis's natural compound was successfully performed against SARS-CoV-2 main protease (Mpro) and spike protein subunit 2 (S2). Initially, the propolis's protein was screened using chromatography analysis and successfully identified 22 compounds in the propolis. Four compounds were further investigated, i.e., neoblavaisoflavone, methylophiopogonone A, 3′-Methoxydaidzin, and genistin. The binding affinity of 3′-Methoxydaidzin was −7.7 kcal/mol, which is similar to nelfinavir (control), while the others were −7.6 kcal/mol. However, we found the key residue of Glu A:166 in the methylophiopogonone A and genistin, even though the predicted binding energy slightly higher than nelfinavir. In contrast, the predicted binding affinity of neoblavaisoflavone, methylophiopogonone A, 3′-Methoxydaidzin, and genistin against S2 were −8.1, −8.2, −8.3, and −8.3 kcal/mol, respectively, which is far below of the control (pravastatin, −7.3 kcal/mol). Instead of conventional hydrogen bonding, the π bonding influenced the binding affinity against S2. The results reveal that this is the first report about methylophiopogonone A, 3′-Methoxydaidzin, and genistin as candidates for anti-viral agents. Those compounds can then be further explored and used as a parent backbone molecule to develop a new supplementation for preventing SARS-CoV-2 infections during COVID-19 outbreaks.