Background: The use of sports supplements among adolescents is rising globally, driven by fitness trends and social media influence, yet knowledge gaps persist. This study aimed to assess supplement usage patterns, knowledge, attitudes, information sources, and the impact of a social media educational intervention among Serbian secondary school students. Methods: A single-group pre–post educational intervention study was conducted in secondary school students (aged 14–18) in Vojvodina, Serbia. A 21-question anonymous questionnaire was distributed to 1000 students along with parental informed consent forms. Pre-intervention survey assessed sociodemographics, physical activity and social media habits, supplement use information sources, and awareness of risks and banned substances. Based on the initial findings, an educational campaign delivered 56 short videos (≈70 s each) on Instagram and TikTok covering most frequently used supplements (e.g., creatine, proteins, caffeine, energy drinks). After, the intervention survey was repeated. The data were analyzed using the McNemar–Bowker test of symmetry. Results: In this study, 65% of Serbian secondary school adolescents reported being physically active, engaging predominantly in gym workouts and team sports. The majority of participants initiate dietary supplement use independently, without consulting healthcare professionals or adults. The most commonly used supplements were vitamins and minerals, while energy drinks ranked notably high. Social media intervention had a limited impact due to its short duration; however, certain changes were detected. Conclusions: Serbian adolescents frequently use sports supplements without adequate professional guidance. Long-term TikTok/Instagram interventions could be used in the future in order to influence behaviors and improve knowledge about sport supplement use.

Possible sustainable resources of beneficial compounds for various applications are agricultural crop and food production residues (ACFPR), which are supported by considerable efforts to characterize their compositions and biological activities. This knowledge can be utilized for the rational selection of agricultural crop residue extracts and their components and possible use in the development of value‐added products, such as anti‐ageing cosmetics. The appearance of wrinkles, pigmentation, and a reduction in skin elasticity are typical signs of ageing skin that are often alleviated by natural product‐based preparations. Here, we use in silico approaches to identify natural compounds from agricultural crop and food production residues with the potential to alleviate symptoms of or reverse the skin ageing process. Target predictions combined with extensive database and literature searches were utilized to identify compounds present in ACR and proteins linked to skin ageing. The binding affinity of natural products to selected proteins using molecular docking and the respective intermolecular interaction analyses are predicted to provide an indicative measure of the compounds' potential for skin anti‐ageing activity. A number of natural compounds with the potential to interact with protein targets such as collagenase, elastase, and hyaluronidase were identified. In addition to in silico findings, cytotoxicity assays were conducted using rose hip seed extracts against Hs294T (human metastatic melanoma, ATCC HTB‐140) and MRC‐5 (normal fetal lung fibroblasts, ATCC CCL 171), demonstrating selective cytotoxicity. ELISA assays revealed that rose hip seed extracts induced a significant increase in SIRT1 levels (160% of control) and a reduction in TGF‐β levels (80% of control). These experimental results support the potential of agricultural crop residue extracts in modulating key proteins involved in skin ageing, reinforcing their viability as ingredients in anti‐ageing cosmetic formulations. An analysis of the molecular relationships and pathways that organic substances from sustainable sources can affect offers the potential for developing formulations for skin rejuvenation with possible synergistic effects by utilizing the rational design of innovative skincare products and laying the framework for more effective screening of anti‐ageing compounds for different applications.

Black raspberry (Rubus occidentalis L., Rosaceae) is an important source of polyphenolic compounds, particularly anthocyanins and ellagitannins, which are associated with significant pharmacological activities. Due to its nutritional and therapeutic potential, this species is cultivated in many temperate regions worldwide, including Serbia. This study aimed to conduct a comparative analysis of the chemical composition and in vitro pharmacological activities of lyophilized infusion and decoction of black raspberry fruit. Spectrophotometric analyses revealed differences in the content of total phenolics (56.49 and 60.81 mg GAE/g), tannins (9.90 and 15.26 mg GAE/g), and anthocyanins (24.29 and 4.81 mg CGE/g) for infusion and decoction, respectively. HPLC analysis revealed anthocyanins (cyanidin-3-sambubioside, cyanidin-3-glucoside, and cyanidin-3-rutinoside) as the predominant polyphenolic compounds, along with phenol carboxylic acids (protocatechuic acid and ellagic acid), flavanol (catechin), and flavonols (rutin and isoquercitrin). Regarding pharmacological activities, black raspberry fruit teas showed pronounced antioxidant activity, confirmed by DPPH (IC50: 98.62 and 95.74 μg/mL), ABTS (IC50: 25.00 and 31.74 μg/mL), and FRAP (1.25 and 1.05 mmol Fe²⁺/g) assays for infusion and decoction, respectively. Significant inhibitory effects were also observed against α-amylase (IC50: 2.17 and 1.89 mg/mL), α-glucosidase (IC50: 57.77 and 31.53 μg/mL), and pancreatic lipase (IC50: 1.64 and 1.46 mg/mL). These findings suggest that black raspberry fruit teas are a valuable source of polyphenolic compounds with promising antioxidant, hypoglycemic, and hypolipidemic properties, supporting their potential application as functional beverages and natural therapeutic agents.