The importance of polarization aberrations has been recognized and studied in numerous optical systems and related applications. It is known that polarization aberrations are particularly crucial in certain photogrammetry and microscopy techniques that are related to vectorial information—such as polarization imaging, stimulated emission depletion microscopy, and structured illumination microscopy. Hence, a reduction in polarization aberrations would be beneficial to different types of optical imaging/sensing techniques with enhanced vectorial information. In this work, we first analyzed the intrinsic polarization aberrations induced by a high-NA lens theoretically and experimentally. The aberrations of depolarization, diattenuation, and linear retardance were studied in detail using the Mueller matrix polar-decomposition method. Based on an analysis of the results, we proposed strategies to compensate the polarization aberrations induced by high-NA lenses for hardware-based solutions. The preliminary imaging results obtained using a Mueller matrix polarimeter equipped with multiple coated aspheric lenses for polarization-aberration reduction confirmed that the conclusions and strategies proposed in this study had the potential to provide more precise polarization information of the targets for applications spanning across classical optics, remote sensing, biomedical imaging, photogrammetry, and vectorial optical-information extraction.

The characterization and evaluation of skin tissue structures are crucial for dermatological applications. Recently, Mueller matrix polarimetry and second harmonic generation microscopy have been widely used in skin tissue imaging due to their unique advantages. However, the features of layered skin tissue structures are too complicated to use a single imaging modality for achieving a comprehensive evaluation. In this study, we propose a dual-modality imaging method combining Mueller matrix polarimetry and second harmonic generation microscopy for quantitative characterization of skin tissue structures. It is demonstrated that the dual-modality method can well divide the mouse tail skin tissue specimens’ images into three layers of stratum corneum, epidermis, and dermis. Then, to quantitatively analyze the structural features of different skin layers, the gray level co-occurrence matrix is adopted to provide various evaluating parameters after the image segmentations. Finally, to quantitatively measure the structural differences between damaged and normal skin areas, an index named Q-Health is defined based on cosine similarity and the gray-level co-occurrence matrix parameters of imaging results. The experiments confirm the effectiveness of the dual-modality imaging parameters for skin tissue structure discrimination and assessment. It shows the potential of the proposed method for dermatological practices and lays the foundation for further, in-depth evaluation of the health status of human skin.

Mueller matrix polarimetry is widely used in biomedical studies and applications, for it can provide abundant microstructural information about tissues. Recently, several methods have been proposed to decompose the Mueller matrix into groups of parameters related to specific optical properties which can be used to reveal the microstructural information of tissue samples more clearly and quantitatively. In this study, we thoroughly compare the differences among the parameters derived from the Mueller matrix polar decomposition (MMPD) and Mueller matrix transformation (MMT), which are two popular methods in tissue polarimetry studies and applications, while applying them on different tissue samples for both backscattering and transmission imaging. Based on the Mueller matrix data obtained using the setups, we carry out a comparative analysis of the parameters derived from both methods representing the same polarization properties, namely depolarization, linear retardance, fast axis orientation and diattenuation. IN particular, we propose several modified MMT parameters, whose abilities are also analyzed for revealing the information about the specific type of tissue samples. The results presented in this study evaluate the applicability of the original and modified MMT parameters, then give the suggestions for appropriate parameter selection in tissue polarimetry, which can be helpful for future biomedical and clinical applications.