The incorporation of hydrophobic ingredients, such as resveratrol (a fat-soluble phytochemical), in nanoemulsions can increase the water solubility and stability of these hydrophobic ingredients. The nanodelivery of resveratrol can result in a marked improvement in the bioavailability of this health-promoting ingredient. The current study hypothesized that resveratrol can bind to caprine casein, which may result in the preservation of the biological properties of resveratrol. The fluorescence spectra provided proof of this complex formation by demonstrating that resveratrol binds to caprine casein in the vicinity of tryptophan amino acid residues. The caprine casein/resveratrol complex is stabilized by hydrophobic interactions and hydrogen bonds. Hence, to study the rate of resveratrol degradation during processing/storage, resveratrol losses were determined by reversed-phase high performance liquid chromatography (RP-HPLC) in nanoemulsions stabilized by bovine and caprine caseins individually and in combination with polysorbate-20. At 48 h oxidation, 88.33% and 89.08% was left of resveratrol in the nanoemulsions stabilized by caprine casein (α_s1-I)/polysorbate-20 complex and caprine (α_s1-II)/polysorbate-20 complex, while there was less resveratrol left in the nanoemulsions stabilized by bovine casein/polysorbate-20 complex, suggesting that oxygen degradation was involved. The findings of this study are crucial for the food industry since they imply the potential use of caprine casein/polysorbate-20 complex to preserve the biological properties of resveratrol.

Lutein is an important antioxidant that quenches free radicals. The stability of lutein and hence compatibility for food fortification is a big challenge to the food industry. Encapsulation can be designed to protect lutein from the adverse environment (air, heat, light, pH). In this study, we determined the impact of mixed biopolymer systems based on bovine and caprine caseins, yeast β-glucan, and maltodextrin as wall systems for microencapsulating lutein dispersed in emulsified lipid carriers by spray drying. The performance of these wall systems at oil/water interfaces is a key factor affecting the encapsulation of lutein. The highest encapsulation efficiency (97.7%) was achieved from the lutein microcapsules prepared with the mixed biopolymer system of caprine α_s1-II casein, yeast β-glucan, and maltodextrin. Casein type and storage time affected the stability of lutein. The stability of lutein was the highest (64.57%) in lutein microcapsules prepared with the mixed biopolymer system of caprine α_s1-II casein, yeast β-glucan, and maltodextrin, whereas lutein microcapsules prepared with the biopolymer system of bovine casein, yeast β-glucan, and maltodextrin had the lowest (56.01%). The stability of lutein in the lutein microcapsules dramatically decreased during storage time. The antioxidant activity of lutein in the lutein microcapsules was closely associated with the lutein concentration.

The fungus Monosporascus cannonballus is a pathogen that causes a severe disease called vine decline, which is found in areas where melons and watermelons are cultivated worldwide. Nevertheless, few studies have examined the hormones involved in host-pathogen interactions. Thus, this study was conducted to identify and quantify these metabolites. The varieties USA PI 124,104 (resistant) and TAM-Uvalde (susceptible) were inoculated with the pathogen, and the hormones were quantified before inoculation (0 h) and 24, 48, and 72 h after inoculation employing ultra-performance liquid chromatography analysis. The hormone levels varied over time. Peculiarly, gibberellic acid and kinetin were detected 24 and 48 h after inoculation, which may be indicative of their synthesis by the fungus. Likewise, methyl jasmonate was detected 48 h after inoculation, a time at which the pathogen could also have produced it. Additionally, salicylic and jasmonic acids were detected, and their concentrations varied antagonistically over time. Interestingly, jasmonic acid could also have been synthesized by the fungus, suggesting a hemibiotrophic lifestyle. Hence, further studies are needed to identify the origins of kinetin, methyl jasmonate, gibberellic and jasmonic acids.