Soybean is an important cash crop in the world, and drought is the main reason for the loss of soybean plants productivity, with drought stress during the most water-sensitive flowering period of soybeans. In this article, drought-tolerant variety Heinong 44 (HN44) and drought-sensitive variety Heinong 65 (HN65) were used as experimental materials. Drought treatment was carried out at the early flowering stage. The method of controlling soil moisture content was used to simulate different degrees of drought, and the physiological changes of these two varieties of soybean under different soil moisture contents were studied. The results showed that with a decrease in soil moisture content, the content of malondialdehyde (MDA) in soybean leaves increased significantly; the activities of peroxidase (POD), catalase (CAT), and ascorbic acid peroxidase (APX) increased first and then decreased; the content of proline, soluble sugar, and soluble protein increased; and the total antioxidant capacity (T-AOC) increased significantly. When the soil moisture content was 15.5%, the degree of membrane lipid peroxidation, osmotic regulatory substances, antioxidant enzyme activity, and T-AOC increased the most, and the decrease in drought-tolerant variety HN44 was significantly less than that of drought-sensitive variety HN65. Our research reveals the response law of soybean crops to physiological characteristics under water deficit and provides theoretical basis and guiding significance for drought-resistant cultivation and breeding of soybean.

Drought is one of the major abiotic stress factors limiting soybean growth and yield, and it frequently occur globally. Therefore, exploring resistant varieties from soybean germplasm is important under climate change. To screen drought resistant spring soybean varieties at seedling stage, pot experiment was used to detect the Survival percentage after drought stress of 60 soybean varieties at seedling stage, twice drought rehydration treatments on seedlings, to evaluate the drought tolerance of spring soybean. The results showed that at the seedling stage, seven varieties were considered drought tolerant, 17 varieties were considered drought sensitive, and 36 varieties were considered to be moderately drought tolerant. Based on this experiment, number 44 (heinong37), 48 (heinong44), 49 (heinong45), 52 (heinong48) is considered the best drought resistant, and number 3 (dongnong48), 4 (dongnong52), 27 (suinong25), 60 (heinong65) is the most sensitive. These varieties provide a reference for further study on drought tolerance and stress resistance gene screening of soybean at the molecular level. The selected soybean varieties can be planted in areas with suitable climates and frequent drought to meet the local soybean demand. In other regions, although cannot be directly grown, they can still be used as parents of selected varieties or as materials for gene screening and extraction, to assist crop breeding at the molecular level in response to increasingly severe drought stress problems under the current climate trends.