The aim of this research was to determine the optimal medium for Exopolysaccharides (EPS) production by a Lactobacillus delbrueckii subsp. bulgaricus ropy strain isolated from a locally produced commercial fermented milk, in reconstituted skim milk (RSM) 10% (w/v), milk whey (MW), and soy milk whey (SMW), under optimal growth conditions for this strain. Milk whey was made by coagulating fresh milk using papaya latex 3% (v/v); soy milk whey was obtained from tofu household industry. The chemical composition of the substrate media was determined by proximate analysis, and sterilization was accomplished in an autoclave at 121°C for 15 min. Culture media were inoculated with 1% (v/v) of a starter culture of L. delbrueckii subsp. bulgaricus and then incubated at 30°C for 16 hr. EPS production, lactic acid content, cell counting, and pH were determined after the media were cooled at 5°C. Findings showed that on the basis of the growth characteristics of L. delbrueckii subsp. bulgaricus, the best medium for EPS production was RSM 10% (258.60 ± 26.86 mg/L) compared to the milk whey (69.60 ± 9.48 mg/L) and soy milk whey (49.80 ± 9.04 mg/L).

The objective of this study was to investigate yeast characteristics obtained from commercial grain kefir from Indonesian. Isolation based on macroscopic morphology and microscopic morphology. The first method of research is activation of kefir grain using 10% reconstitution milk, yeast growth on Potato Dextrose Agar (PDA-Agar) medium, yeast coloration by using Lactophenol cotton blue. Then, yeast identification was done by macroscopic and microscopic morphology. Macroscopic morphological observations are observations of colony morphology at the time of isolation and purification, including size, shape, texture, color, surface, elevation, and edges. Microscopic morphological observations include cell shape, budding (budding) which first make preparations with yeast coloring then observed with Zeiss Asio Imager A2 Microscope using Zeiss Axiocam HRC camera. Macroscopic observation of yeast size description colony very small, small, medium, large, colony form is round, the margin is raised, the elevation is entire, the texture is smooth and surface glistening, cream colony color, and yeast smell characteristic. Microscopic observation seen there is cell nucleus, oval, there is pseudohypa, budding, gram-positive, urea test negative, glucose, lactose, maltose fermentation test positive, sucrose fermentation test negative, growth test on liquid media growth in surface medium (pellicle), and bottom medium (sediment). Based on the morphological observations in macroscopic and microscopic yeast identified genus Saccharomyces.

Staphylococcus aureus causes foodborne disease in eggs. It is characteristic of pathogenicity and harmful to human health if contaminated eggs and products are contaminated from pre-production at the manufacturer until post-production. S. aureus is a Gram-positive bacterium normally present in the skin and mucosa of humans and other animals. Methicillin-resistant S. aureus (MRSA) is a strain of S. aureus that is resistant to several types of antibiotics, including tetracycline, amoxicillin, ampicillin, ciprofloxacin, ceftriaxone, beta-lactam, and azithromycin. Impure S. aureus infections in eggs and processed egg products have been reported in Indonesia for for 5 years. However, only a limited number of cases have been reported. Based on the Indonesian National Standard, S. aureus contamination in fresh poultry eggs is limited to <1×102 colony forming unit/g, eggs processed from salty eggs as much as <1 × 101 colonies/g, and food processed from base eggs, e.g., custard a must negative/gram. One Health is an approach to collaborative cross-recognized sector linkages between human health, animal health, and the environment, which should be managed holistically. It is expected that one health approach will be able to prevent and control the risk of S. aureus contamination in eggs and processed egg proucts. This review describes the incidence of S. aureus and MRSA in Indonesia compared with other countries. This review provides information on the One Health approach for preventing and controlling S. aureus contamination of eggs in Indonesia so that it can be adopted elsewhere. Keywords: eggs and egg products, foodborne, Indonesia, methicillin-resistant Staphylococcus aureus.

Background: The recent deforestation for agricultural, mining, and human re-settlement has significantly reduced the habitat of many non-human primates (NHPs) in Indonesia and intensifies interaction between the NHPs and humans and thus opening the possibility of pathogen spill-over. The emergence of zoonotic malaria, such as Plasmodium knowlesi, poses an immense threat to the current malaria control and elimination that aims for the global elimination of malaria by 2030. As malaria in humans and NHPs is transmitted by the female Anopheles mosquito, malaria vector control is very important to mitigate the spill-over of the malaria parasite to humans. The present study aims to explore the Anopheles species diversity in human settlements adjacent to the wildlife sanctuary forest in Buton Utara Regency, Southeast Sulawesi, Indonesia, and identify the species that potentially transmit the pathogen from monkey to human in the area. Methods: Mosquito surveillance was conducted using larval and adult collection, and the collected mosquitoes were identified morphologically and molecularly using the barcoding markers, cytochrome oxidase subunit I (COI), and internal transcribed species 2 (ITS2) genes. Plasmodium sporozoite carriage was conducted on mosquitoes collected through human landing catch (HLC) and human-baited double net trap (HDNT). Results: The results revealed several Anopheles species, such as Anopheles flavirostris (16.6%), Anopheles sulawesi (3.3%), Anopheles maculatus (3.3%), Anopheles koliensis (1.2%), and Anopheles vagus (0.4%). Molecular analysis of the sporozoite carriage using the primate-specific malaria primers identified An. sulawesi, a member of the Leucosphyrus group, carrying Plasmodium inui sporozoite. Conclusions: This study indicates that the transmission of zoonotic malaria in the area is possible and alerts to the need for mitigation efforts through a locally-tailored vector control intervention and NHPs habitat conservation.

Exopolysaccharide (EPS) is an extracellular slime polysaccharide from bacteria and one of EPS produced by Alcaligenesfaecalis var. myxogenes strain 10C3 which found by Harada for the first time in 1965. In this study, it was used Lactobacillus delbrueckii subsp. bulgaricus B-5b, which obtained from Japan Milk Product Technology Association, Tokyo, Japan. During the course of the investigation, the culture was routinely propagated in 10% RSM. The RSM was added with 0, 0.5 and 1% of EPS, autoclaved at 121°C for 15 min and tempered to 37°C prior to inoculation. A 0,1% inoculum was added to the RSM and the culture was allowed to incubate at 37°C overnight. Milk curd investigated toward growth curve of cell bacteria numbers, pH, acidity, viscosity, rheometric properties. The result indicated that increasing EPS concentration cause of increases the pH and the pH of milk increases its viscosity perhaps by swelling of casein micelles, but a more drastic pH decreases causes the viscosity to increase, which is caused by aggregation of casein. On the other side, increasing the EPS concentration decreases acidity as expected. It is well established that milk which has been heated at a temperature above 70°C has a longer coagulation time and forms a weaker curd than the original unheated milk.

The study was conducted to determine the effect of Lactobacillus sp. in intestinal histology, total amount of Escherichia coli in broiler chicken excreta and broiler performance. Research has been conducted using a completely randomized design with 4 treatments and 5 replications utilize 15 chickens. Lactobacillus sp. 5.8 x 107 CFU/mL was mixed in drinking water and also feed commercial concentrates together ad libitum. The results showed that treatment had significant effect (P 0.05) ration consumption on doses of Lactobacillus sp. treatment 0 mL/days, 1 ml/days, 3 ml/days, and 5 ml/days. In general, the probiotic treatments of 1, 3, and 5 mL/days of lactobacillus sp. were better at villus height, villus surface area, weight gain, final weight, and feed conversion compared to control. Treatment a dose of 3 mL / d (1.7 x 108 CFU) is the best dose in providing optimal response to histology of the small intestine, and broiler performance. Treatment 5 mL/days has the lowest amount of Escherichia coli in manure of broiler. In conclusion, Lactobacillus sp. can be used as one candidate probiotic for broiler.