Since the advent of biosensing, structuring of electrode surfaces for the improvement of cell‐coupling and electrophysiological properties has been extensively investigated. Most of these methods result in structures with predefined dimensions and regular organization. Nevertheless, natural adhesion surfaces of the cells are hardly uniform. Therefore, this study focusses on fabricating randomly organized nanostructures mimicking the irregular distribution of natural collagen fibers coated on a planar surface. Fiber geometries are replicated by using a spin‐coating process followed by thermal nanoimprint lithography and gold electroplating. Microscopic studies reveal the width of these biomimetic collagen‐like gold nanostructures ranging between 200 nm and 5 µm, with a uniform height of ≈35 nm. In comparison to unstructured gold surfaces, nanostructured surfaces display a decrease in impedance magnitude by 50% for frequencies below 1 kHz and show an increase in critical free surface energy by 35%, the latter translating to an increased surface wettability. Culturing enteric neurons from postnatal mice, a relative hard to handle type of neurons, results in an improved spreading of the neural networks on the nanostructured surfaces.

Tip-enhanced Raman spectroscopy (TERS) is an emerging technique with a large number of potential applications in different fields such as chemistry, physics, biology, life sciences, and materials science. TERS in liquids offers nondestructive and label-free topographical and chemical imaging of molecules and chemical reactions under native conditions at a nanometer scale. It combines Raman spectroscopy with scanning probe microscopy, providing a unique combination of chemical information and high spatial resolution. The versatility of TERS increases even further if it is modified for simultaneous electrochemical measurements. This paper reviews the recent progress of TERS in liquid, with a focus on molecular structures, interfaces, and chemical reactions studied to date. For example, TERS studies revealed nanoscale chemical images of various molecules containing a benzene ring such as thiophenol or carbon nanotubes. Additionally, heterogeneous catalytic reactions at the solid–liquid interface were investigated in a new way to gain a deeper insight into electrochemical processes. Furthermore, TERS measurements expanded our knowledge of several redox compounds and enabled new insights into various chemical transformations. Despite substantial progress, TERS in liquids is not yet a routine measurement method, and there are still several challenges to solve before TERS can unleash its full potential.