The recent outbreak of respiratory syndrome-coronavirus-2 (SARS-CoV-2), which causes coronavirus disease (COVID-19), has led to the widespread use of therapeutics, including dexamethasone (DEXA). DEXA, a synthetic glucocorticoid, is among the widely administered drugs used to treat hospitalized COVID-19 patients. The global COVID-19 surge in infections, consequent increasing hospitalizations, and other DEXA applications have raised concerns on eminent adverse ecological implications to aquatic ecosystems. Here, we aim to summarize published studies on DEXA occurrence, fate, and effects on organisms in natural and engineered systems as, pre-COVID, the drug has been identified as an emerging environmental contaminant. The results demonstrated a significant reduction of DEXA in wastewater treatment plants, with a small portion, including its transformation products (TPs), being released into downstream waters. Fish and crustaceans are the most susceptible species to DEXA exposure in the parts-per-billion range, suggesting potential deleterious ecological effects. However, there are data deficits on the implications of DEXA to marine and estuarine systems and wildlife. To improve DEXA management, toxicological outcomes of DEXA and formed TPs should entail long-term studies from whole organisms to molecular effects in actual environmental matrices and at realistic exposure concentrations. This can aid in striking a fine balance of saving human lives and protecting ecological integrity.

Antiretroviral (ARVs) drugs are used to manage the human immunodeficiency virus (HIV) disease and are increasingly being detected in the aquatic environment. However, little is known about their effects on non-target aquatic organisms. Here, Daphnia magna neonates were exposed to Efavirenz (EFV) and Tenofovir (TFV) ARVs at 62.5–1000 µg/L for 48 h in river water. The endpoints assessed were mortality, immobilization, and biochemical biomarkers (catalase (CAT), glutathione S-transferase (GST), and malondialdehyde (MDA)). No mortality was observed over 48 h. Concentration- and time-dependent immobilization was observed for both ARVs only at 250–1000 µg/L after 48 h, with significant immobilization observed for EFV compared to TFV. Results for biochemical responses demonstrated that both ARVs induced significant changes in CAT and GST activities, and MDA levels, with effects higher for EFV compared to TFV. Biochemical responses were indicative of oxidative stress alterations. Hence, both ARVs could potentially be toxic to D. magna.

Cerium oxide engineered nanoparticles (nCeO₂) are widely used in various applications and are, also, increasingly being detected in different environmental matrixes. However, their impacts on the aquatic environment remain poorly quantified. Hence, there is a need to investigate their effects on non-target aquatic organisms. Here, we evaluated the cytotoxic and genotoxic effects of <25 nm uncoated-nCeO₂ on algae Pseudokirchneriella subcapitata. Apical (growth and chlorophyll a (Chl a) content) and genotoxic effects were investigated at 62.5–1000 µg/L after 72 and 168 h. Results demonstrated that nCeO₂ induced significant growth inhibition after 72 h and promotion post 96–168 h. Conversely, nCeO₂ induced enhanced Chl a content post 72 h, but no significant changes were observed between nCeO₂–exposed and control samples after 168 h. Hence, the results indicate P. subcapitata photosynthetic system recovery ability to nCeO₂ effects under chronic-exposure conditions. RAPD-PCR profiles showed the appearance and/or disappearance of normal bands relative to controls; indicative of DNA damage and/or DNA mutation. Unlike cell recovery observed post 96 h, DNA damage persisted over 168 h. Thus, sub-lethal nCeO₂-induced toxicological effects may pose a more serious threat to algae than at present anticipated.

The study investigated the interactions of coated-gold engineered nanoparticles (nAu) with the aquatic higher plant Salvinia minima Baker in 2,7, and 14 d. Herein, the nAu concentration of 1000 µg/L was used; as in lower concentrations, analytical limitations persisted but >1000 µg/L were deemed too high and unlikely to be present in the environment. Exposure of S. minima to 1000 µg/L of citrate (cit)- and branched polyethyleneimine (BPEI)-coated nAu (5, 20, and 40 nm) in 10% Hoagland’s medium (10 HM) had marginal effect on biomass and growth rate irrespective of nAu size, coating type, or exposure duration. Further, results demonstrated that nAu were adsorbed on the plants’ roots irrespective of their size or coating variant; however, no evidence of internalization was apparent, and this was attributed to high agglomeration of nAu in 10 HM. Hence, adsorption was concluded as the basic mechanism of nAu accumulation by S. minima. Overall, the long-term exposure of S. minima to nAu did not inhibit plant biomass and growth rate but agglomerates on plant roots may block cell wall pores, and, in turn, alter uptake of essential macronutrients in plants, thus potentially affecting the overall ecological function.