The fungus Coleosporium zanthoxyli causes leaf rust in Chinese pepper (Zanthoxylum armatum). To investigate the control effect of elicitor treatment on leaf rust in this species, the impact of salicylic acid (SA) on the spores and growth of C. zanthoxyli and the induced resistance to leaf rust by Z. armatum were analyzed, and the possible defense mechanisms involved in SA induction were evaluated. The results showed that SA had no effect on C. zanthoxyli spore germination and growth; however, rust resistance was induced in Z. armatum. The optimal SA treatment concentration was 0.4 mg/ml, and the relative cure effect reached 44.56%. SA-induced disease resistance was evident for up to 10 days, while the optimal induction interval was 48 h after stimulation. Consistent with the induced resistance, H₂O₂, total protein, total phenol, and lignin concentrations and polyphenol oxidase (PPO), peroxidase (POD), phenylalanine ammonia lyase (PAL), superoxide dismutase (SOD), and catalase (CAT) activities were significantly increased with the SA treatment, whereas the malondialdehyde content was significantly decreased. In addition, exogenous SA promoted defense-related enzyme activities, including those of POD, CAT, and PAL, and increased H₂O₂, lignin, and endogenous SA contents. Furthermore, SA induced the expression of SA signaling pathway genes such as ZaPR1 and ZaNPR1, and silencing ZaPR1 enhanced the sensitivity of Z. armatum to leaf rust. Our results demonstrated that 0.4 mg/ml SA priming increased the activities of CAT, POD, and PAL, elevated the contents of H₂O₂, lignin, and endogenous SA, and upregulated the expression of the SA-related gene ZaPR1, thereby enhancing the resistance of Z. armatum to leaf rust.

In this study, we investigated the physiological response to low-temperature stress and comprehensively evaluated the cold resistance of green prickly ash germplasms. One-year-old branches of 23 green prickly ash varieties were used as experimental materials, three branches were selected from each variety, and three replicates of single branches were established. Subsequently, the physiological responses of the 23 green prickly ash germplasm resources to low-temperature stress were analyzed based on the relative conductivity (REC), osmotic adjustment substances, and antioxidant enzyme activities. We found that as the temperature decreased, the REC of each germplasm increased. The most rapid REC increase occurred from 0 to −10 °C and continued to gradually gently increase from −10 to −30 °C. The lethal dose-50 temperature (LT₅₀) of the germplasms was between 3.16 and −12.37 °C. The top three cold-resistant varieties were TJ, SCHJ, and CJ, and the last three cold-resistant varieties were EWJ, DYSJ, and HYXJ. The results of a correlation analysis revealed a significant correlation between superoxide dismutase (SOD) activity and REC, between REC and free protein, and between SOD activity and catalase. The results of the PCA revealed that the first category includes 5 pepper varieties of CJ, LFJ, and XYTJ with higher cold-resistance score indexes; the second category includes 13 pepper varieties of YQ2 H, WCFZ, and TZJ with appropriate scores; and the third category includes 5 pepper varieties of HYXJ, HNJ, and DYSJ with lower scores. Cluster analysis was performed to classify the cold-resistance physiological indices, and four groups were obtained. The most cold-resistant groups included CJ and LFHJ, whereas the most cold-sensitive groups included HYXJ, DYSJ, and HNJ. Finality, the subordinate function method was used to determine the cold resistance from strong to weak. The varieties with the strongest cold resistance were LFJ, EWJ, and CJ, and the weakest varieties were DYSJ, YQ1H, and HYXJ. The strongest cold-resistant varieties were LFHJ and CJ, followed by DYSJ, YQ1H, and HYXJ. Based on multiple lines of evidence, the most cold-resistant varieties were CJ and LFJ, while the most cold-sensitive varieties were DYSJ and HYXJ. In conclusion, in this study, we elucidated the low-temperature tolerance of different varieties of green prickly ash, and varieties with high cold resistance were selected. These findings provide theoretical guidance and technical support for the screening of cold-resistant green prickly ash germplasms, which will facilitate their introduction to northern China.

In this study, we prepared high-nitrogen self-doped porous carbons (NPC1 and NPC2) derived from the pruned branches and seeds of Zanthoxylum bungeanum using a simple one-step method. NPC1 and NPC2 exhibited elevated nitrogen contents of 3.56% and 4.22%, respectively, along with rich porous structures, high specific surface areas of 1492.9 and 1712.7 m² g⁻¹ and abundant surface groups. Notably, both NPC1 and NPC2 demonstrated remarkable adsorption abilities for the pollutant methylene blue (MB), with maximum monolayer adsorption capacities of 568.18 and 581.40 mg g⁻¹, respectively. The adsorption kinetics followed the pseudo-second-order kinetics and the adsorption isotherms conformed to the Langmuir isotherm model. The adsorption mechanism primarily relied on the hierarchical pore structures of NPC1 and NPC2 and their diverse strong interactions with MB molecules. This study offers a new approach for the cost-effective design of nitrogen self-doped porous carbons, facilitating the efficient removal of MB from wastewater.

The rhizome is an economically important part of ginger (Zingiber officinale Rosc.). However, the mechanism of ginger rhizome enlargement remains unclear. In this study, we performed an integrated analysis of the hormone content and transcriptome of ginger at three rhizome enlargement stages: initial enlargement (S1), middle enlargement (S2), and peak enlargement (S3). With rhizome enlargement, the levels of the hormones zeatin (ZT), gibberellic acid (GA), indole acetic acid (IAA), and jasmonic acid (JA) were significantly increased, and this increase was positively correlated with rhizome diameter. Transcriptomic analysis identified a large number of differentially expressed genes (DEGs); the number of DEGs were 2,206 in the transition from S1 to S2, and 1,151 in the transition from S2 to S3. The expression of several genes related to hormone biosynthesis and signalling and cell division or expansion, and transcription factors was significantly altered, which suggests that these genes play essential roles in rhizome enlargement. The results of correlation analysis suggested that the process of ginger rhizome enlargement may be primarily related to the regulation of endogenous cytokinin, GA₃, auxin, and JA biosynthesis pathways and signal transduction; GRAS, HB, MYB, MYB122, bZIP60, ARF1, ARF2, E2FB1, and E2FB2, which may regulate the expression of rhizome formation-related genes; and CYC2, CDKB1, CDKB2, EXPA1, and XTH7, which may mediate cell division and expansion. These results provide gene resources and information that will be useful for the molecular breeding in ginger.

A two-year field experiment (2019–2020) was conducted in Yongchuan, Chongqing, and Deyang, Sichuan, to investigate the differences in nitrogen (N) accumulation and grain yield formation between maize varieties with contrasting N efficiencies. Additionally, we clarify the contribution characteristics of N accumulation and distribution to the grain yield of maize in Southwest China. Two maize varieties—N-efficient Zhenghong 311 (ZH 311) and N-inefficient Xianyu 508 (XY 508)—were tested under four N levels (0, 120, 240, and 360 kg·ha-1). Kernel number per ear showed the strongest correlation with N accumulation (R2 = 0.8186) and weakest correlation with 100-kernel weight (R2 = 0.2479). ZH 311 exhibited a significantly higher ear row number, kernel number per row, total kernel number per ear, and grain yield than XY 508. However, its 100-kernel weight was lower, indicating that N accumulation primarily regulates grain yield by modulating the kernel number per ear. N accumulation peaked during the V12–R1 (12-leaf to silking) stages, with substantial contributions from the SS–V6 (emergence to 6-leaf; R2 = 0.7737), V12–R1 (R2 = 0.7129), and R1–R3 (silking to milking stage; R2 = 0.7277) stages. Root (R2 = 0.6804), stem-sheath (R2 = 0.8440), and leaf (R2 = 0.8590) N accumulation was positively correlated with grain yield. Root N accumulation contributed the most to yield at the R3 stage, whereas stem-sheath N accumulation had a highly significant contribution in the V12, R1, and R3 stages and leaf N accumulation in the V6, V12, and R3 stages. ZH 311 exhibited significantly higher N accumulation at all growth stages, ear row number, kernel number per row, total kernel number per ear, and grain yield compared to XY 508, while its 100-kernel weight was lower. The N-efficient variety ZH 311 increased grain yield by enhancing kernel number per ear in the early stages (thereby expanding sink capacity) and later strengthening the supply from source organs (stems, leaves, and roots) to ensure assimilate availability.