In order to reduce vehicle vibration during driving conditions, a fuzzy sliding mode control strategy (FSMC) for semi-active air suspension based on the magnetorheological (MR) damper is proposed. The MR damper used in the semi-active air suspension system was tested and analyzed. Based on the experimental data, the genetic algorithm was used to identify the parameters of the improved hyperbolic tangent model, which was derived for the MR damper. At the same time, an adaptive neuro fuzzy inference system (ANFIS) was used to build the reverse model of the MR damper. The model of a quarter vehicle semi-active air suspension system equipped with a MR damper was established. Aiming at the uncertainty of the air suspension system, fuzzy control was used to adjust the boundary layer of the sliding mode control, which can effectively suppress the influence of chattering on the control accuracy and ensure system stability. Taking random road excitation and impact road excitation as the input signal, the simulation analysis of passive air suspension, semi-active air suspension based on SMC and FSMC was carried out, respectively. The results show that the semi-active air suspension based on FSMC has better vibration attenuating performance and ride comfort.