Ahmed Gabr

Prof. Ahmed Gabr

Professor
orcid

Research Focus & Metrics

Academic Career Length

15Years (2012-Present)

(Calculated once Scilit publication data is synced: current year − year of first publication + 1)

Representative Publications

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1 March 2013 in Food Research International
Verified
Journal Article
Closed access
KRKavitha Ravichandran
Kavitha Ravichandran
NSNay Min Min Thaw Saw
Nay Min Min Thaw Saw
Adel Abdelrazek Abdelazim MohdalyAdel Abdelrazek Abdelazim Mohdaly
Adel Abdelrazek Abdelazim Mohdaly
AGAhmed Gabr
Ahmed Gabr
AKAnja Kastell
Anja Kastell
Iryna SmetanskaIryna Smetanska
Iryna Smetanska

12 May 2012 in Journal of Food Science and Technology
Verified
Journal Article
Closed access
PDF file
KRKavitha Ravichandran
Kavitha Ravichandran
RPRavichandran Palaniraj
Ravichandran Palaniraj
NSNay Min Min Thaw Saw
Nay Min Min Thaw Saw
AGAhmed Gabr
Ahmed Gabr
AAAbdelrahman R. Ahmed
Abdelrahman R. Ahmed
Iryna SmetanskaIryna Smetanska
Iryna Smetanska

15 April 2016 in Plant Cell, Tissue and Organ Culture (PCTOC)
Verified
Journal Article
Closed access
PDF file
AGAhmed Gabr
Ahmed Gabr
Hoda B MabrokHoda B Mabrok
Hoda B Mabrok
KGKadry Z. Ghanem
Kadry Z. Ghanem
MBMichael Blaut
Michael Blaut
Iryna SmetanskaIryna Smetanska
Iryna Smetanska

1 December 2016 in Journal of Genetic Engineering and Biotechnology
Verified
Journal Article
Open access
AGAhmed Gabr
Ahmed Gabr
HGHassan Ghareeb
Hassan Ghareeb
Hattem Mohamed El-ShabrawiHattem Mohamed El-Shabrawi
Hattem Mohamed El-Shabrawi
Iryna SmetanskaIryna Smetanska
Iryna Smetanska
SBS.A. Bekheet
S.A. Bekheet
In the present study, the effects of the metabolite elicitors chitosan, methyl jasmonate (MeJA) and salicylic acid (SA) as well as the hairy root transformation were tested for silymarin and phenolic compound accumulation in in vitro cultures of Milk thistle. For callus induction, leaf explants were cultured on MS medium supplemented with 5 mg/l NAA + 2 mg/l Kin + 0.1 mg/l GA3. Chitosan, SA and MeJA were added separately in three concentrations 200, 400 and 800 mg/l; 10, 20 and 40 mg/l; 20, 40 and 80 mg/l, respectively, to hormone free B5 medium. Alternatively, cotyledons of 12 day old seedlings were transformed with Agrobacterium rhizogenes A4 strain. Overall, increasing the concentrations of the three elicitors dramatically increased the total silymarin content. Remarkably, the elicitors mainly enhanced the accumulation of silybine A&B that were not detected in un-treated callus culture (control). In addition, the hairy root culture triggered the accumulation of silybine A&B, and silydianin, which was not detected in the non-transgenic roots. The hairy root culture was superior in production of the phenolic compounds in comparison to the control and elicitor treatments. The hairy root cultures showed also higher antioxidant capacities than non-transformed cultures and/or chemically elicited-callus cultures. Thus hairy root provide instrumental in enhancing the production of economically valuable metabolite.

20 November 2017 in Natural Product Research
Verified
Journal Article
Closed access
AGAhmed Gabr
Ahmed Gabr
Hoda B MabrokHoda B Mabrok
Hoda B Mabrok
EAEmam A. Abdel-Rahim
Emam A. Abdel-Rahim
MEMohamed K. El-Bahr
Mohamed K. El-Bahr
Iryna SmetanskaIryna Smetanska
Iryna Smetanska
Hairy root culture is a promising alternative method for the production of secondary metabolites. In this study, transformed root of Linum usitatissimum was established using Agrobacterium rhizogenes A4 strain from root cultures for lignans, phenolic acids and antioxidant capacity determination. Total lignin content (secoisolariciresinol diglucoside, secoisolariciresinol and matairesinol) was 55.5% higher in transformed root cultures than in the non-transformed root culture. Secoisolariciresinol was detected in higher concentration (2.107 μmol/g DM) in the transformed root culture than non-transformed culture (1.099 μmol/g DM). Secoisolariciresinol diglucoside and matairesinol were exclusively detected in the transformed root culture, but were not found in the non-transformed root culture. The overall production of phenolic acids in transformed roots was approximately 3.5 times higher than that of the corresponding non-transformed culture. Free radical scavenging DPPH˙ and ABTS˙+ assays showed 2.9-fold and 1.76-fold higher anti-oxidant activity in transformed root culture as compared to non-transformed.

9 April 2019 in Physiology and Molecular Biology of Plants
Verified
Journal Article
Closed access
PDF file
AGAhmed Gabr
Ahmed Gabr
Oksana SytarOksana Sytar
Oksana Sytar
HGHassan Ghareeb
Hassan Ghareeb
Marian BresticMarian Brestic
Marian Brestic