Arbuscular mycorrhizal fungi (AMF) are critical for soil ecosystem services as they enhance plant growth and soil quality via nutrient cycling and carbon storage. Considering the growing emphasis on sustainable agricultural practices, this study investigated the effects of conventional and organic farming practices on AMF diversity, abundance, and ecological functions in maize, pepper, and potato-cultivated soils. Using next-generation sequencing and quantitative PCR, we assessed AMF diversity and abundance in addition to soil health indicators such as phosphorus content, total nitrogen, and soil organic carbon. Our findings revealed that, while no significant differences in soil physicochemical parameters or AMF diversity were observed across farming systems when all crop data were combined, organic farming significantly enhances AMF abundance and fosters beneficial microbial ecosystems. These ecosystems play vital roles in nutrient cycling and carbon storage, underscoring the importance of organic practices in promoting robust AMF communities that support ecosystem services. This study not only deepens our understanding of AMF's ecological roles but also highlights the potential of organic farming to leverage these benefits for improving sustainability in agricultural practices.

Imparting knowledge on agriculture and ecology is important for the preservation of nature. This study suggested the design of a rice–fish mixed farming (RFMF) paddy for urban agriculture and ecological education in Korea. This RFMF paddy supports the growth of rice as well as freshwater fish. ANOVA statistical analysis was conducted, and an RFMF paddy was necessary for urban agriculture/education and confirmed that biodiversity was high. To this aim, the design of a 10 m × 10 m RFMF paddy was suggested. Vegetation, insects, and aquatic invertebrates of the RFMF paddy constituted approximately 40 species more than a conventional paddy. The quality of an actual farm’s soil and water was assessed, and techniques for the co-cultivation of rice and fish are proposed. The soil must comply with the standards of Korean paddy soil, and the water must be in the temperature range of 15 to 35 °C. In the proposed design, approximately 44.0 kg rice can be produced, and catfish can grow up to 30 cm. The study suggested many experiences using rice and freshwater fish. On the basis of our study design, a virtual model of an RFMF paddy was developed in consideration of the accessible space. The development of RFMF paddies in educational institutions can promote biodiversity in cities while providing ecological education regarding aquatic plants and insects.