Climate change represents the main problem for agricultural crops, and the constitution of heat-tolerant genotypes is an important breeder’s strategy to reduce yield losses. The aim of the present study was to investigate the whole genome of a heat-tolerant tomato genotype (E42), in order to identify candidate genes involved in its response to high temperature. E42 presented a high variability for chromosomes 1, 4, 7 and 12, and phylogenetic analysis highlighted its relationship with the wild S. pimpinellifolium species. Variants with high (18) and moderate (139) impact on protein function were retrieved from two lists of genes related to heat tolerance and reproduction. This analysis permitted us to prioritize a subset of 35 candidate gene mapping in polymorphic regions, some colocalizing in QTLs controlling flowering in tomato. Among these genes, we identified 23 HSPs, one HSF, six involved in flowering and five in pollen activity. Interestingly, one gene coded for a flowering locus T1 and mapping on chromosome 11 resides in a QTL region controlling flowering and also showed 100% identity with an S. pimpinellifolium allele. This study provides useful information on both the E42 genetic background and heat stress response, and further studies will be conducted to validate these genes.

The constitution of heat tolerant F₁ hybrids is a challenge to ensure high yield and good fruit quality in the global climate. In the present work, we evaluated 15 genotypes for yield-related traits highly affected by high temperatures (HT). This phenotypic analysis allowed to identify four parental genotypes showing promising yield performances under HT conditions. Two of these genotypes also exhibited good fruit quality traits. A molecular marker analysis was carried out for six resistance genes to pathogens mostly affecting tomatoes. This analysis evidenced the presence of a maximum of three resistant alleles in parental genotypes. Exploring single nucleotide polymorphisms (SNPs) revealed by two high-throughput genotyping platforms allowed identifying additional 12 genes potentially involved in resistance to biotic stress, to be further investigated. Following these considerations, 13 F₁ hybrids were constituted combining the parental genotypes and then evaluated for multiple traits under HT conditions. By estimating a hybrid index based on yield performances, desirable quality and resistance gene, we identified seven hybrids showing the best performances. The promising results obtained in the present work should be confirmed by evaluating the best hybrids selected for additional years and environments before proposing them as novel commercial hybrids that could maintain high performances under HT conditions.

In this study, a new process was proposed to make the yellow tomato puree more sustainable, avoiding any waste during production and including peels and seeds in it. In addition, as raw material tomatoes grown without irrigation and fertilisation procedures were used to increase the sustainability. With respect to a traditional puree, the whole-fruit yellow tomato puree contained more β-carotene and polyphenols, exhibiting greater antioxidant capacity, and showed greater consistency due to the high content of pectin in peels. Moreover, a higher content of glutamic acid, responsible for the umami taste, was detected particularly in products from GiaGiù ecotype. Sensory analysis assessed that whole-fruit yellow tomato puree has good acceptability and consumers preferred it for the texture. In conclusion, the whole-fruit yellow tomato puree is not only a sustainable product but can be also used as an excellent ingredient in food formulation with antioxidant properties.

Climate change is a major concern for agricultural crops, and the selection of tolerant genotypes in response to abiotic stresses represents an important breeding strategy to reduce yield losses. In addition, the continuous development of new and more accurate high-throughput technologies for the analysis of DNA sequences is the key to improve biological understanding and application of biological knowledge. In the present work, 27 tomato genotypes already evaluated for their response under high temperature conditions were sequenced by using the ddRAD sequencing technology. The main goal was to provide genomic data useful for identifying candidate genes and variants to cope with current climate changes. Total genomic DNA was extracted from leaves and sequenced on the HiSeq2500 Illumina instrument. Raw reads of the dataset were processed using different bioinformatics tools to generate a Variant Calling Format (VCF) file. The availability of resources reporting polymorphisms among genomes of different genotypes provides a useful basis for studying tomato tolerance to current climate changes and can be used by researchers and breeders to investigate the molecular response mechanisms and develop new breeding programs, also aided by Marked Assisted Selection (MAS). The raw reads were deposited into SRA database (https://www.ncbi.nlm.nih.gov/sra/PRJNA1137563).