Zinc oxide nanoparticles are one of the most commonly engineered nanomaterials and necessarily enter the environment because of the large quantities produced and their widespread application. Understanding the impacts of nanoparticles on plant growth and development is crucial for the assessment of probable environmental risks to food safety and human health, because plants are a fundamental living component of the ecosystem and the most important source in the human food chain. The objective of this study was to examine the impact of different concentrations of zinc oxide nanoparticles on barley Hordeum vulgare L. seed germination, seedling morphology, root cell viability, stress level, genotoxicity, and expression of miRNAs. The results demonstrate that zinc oxide nanoparticles enhance barley seed germination, shoot/root elongation, and H₂O₂ stress level and decrease root cell viability and genomic template stability and up- and downregulated miRNAs in barley seedlings.

Plants exposed to stress use the variety of gene regulatory mechanisms to achieve cellular homeostasis, including posttranscriptional regulation of gene expression where microRNAs (miRNAs) play a pivotal role. Since various environmental stress factors such as nanoparticles affect crop productivity and quality, the aim of the present study was to evaluate the genotoxicity level and to estimate miRNA expression level and chlorophyll a level in the magnetite (Fe₃O₄) nanoparticle-stressed rocket (Eruca sativa Mill.) seedlings grown in hydroponics. Rocket seedlings were exposed to 1 mg/L, 2 mg/L, and 4 mg/L Fe₃O₄ nanoparticles, and after 5 weeks, seed germination rate, root-shoot elongation, genotoxicity, chlorophyll a, and miRNA expression levels were evaluated. The obtained results indicated that 1 mg/L, 2 mg/L, and 4 mg/L concentrations of Fe₃O₄ nanoparticles induce low genotoxicity and have a positive effect on the growth and development of rocket seedlings and that nanoparticles may improve the ability of plants to stand against environmental stresses.

Sustainable agricultural practices are still essential due to soil degradation and crop losses. Recently, the relationship between plants and nanoparticles (NPs) attracted scientists’ attention, especially for applications in agricultural production as nanonutrition. Therefore, the present research was carried out to investigate the effect of Fe₃O₄ NPs at low concentrations (0, 1, 10, and 20 mg/L) on three genotypes of barley (Hordeum vulgare L.) seedlings grown in hydroponic conditions. Significant increases in seedling growth, enhanced chlorophyll quality and quantity, and two miRNA expression levels were observed. Additionally, increased genotoxicity was observed in seedlings grown with NPs. Generally, Fe₃O₄ NPs at low concentrations could be successfully used as nanonutrition for increasing barley photosynthetic efficiency with consequently enhanced yield. These results are important for a better understanding of the potential impact of Fe₃O₄ NPs at low concentrations in agricultural crops and the potential of these NPs as nanonutrition for barley growth and yield enhancement. Future studies are needed to investigate the effect of these NPs on the expression of resistance-related genes and chlorophyll synthesis-related gene expression in treated barley seedlings.

Metal nanoparticles (NPs) have an influence on plant growth and development. They can alter plant shoot and root length, fresh biomass production, and even influence the genome. Nanoparticles are also able to affect expression levels of plant microRNAs. MicroRNAs are able to protect plants from biotic stress, including pathogens which cause powdery mildew. In this study, Hordeum vulgare L. varieties Marthe and KWS Olof were grown in hydroponics with magnetic iron oxide (Fe₃O₄) and copper oxide (CuO) NPs added at 17, 35, and 70mg/L. Plant morphology, genotoxicity, and expression of miR156a were investigated. The Fe₃O₄ and CuO NPs demonstrated different effects on the barley varieties, namely, Fe₃O₄ nanoparticles increased plant shoot and root lengths and fresh biomass, while CuO nanoparticles decreased them. CuO NPs presence caused larger changes on barley genome compared to Fe₃O₄ NPs. Thus, Fe₃O₄ NPs reduced genome stability to 72 in the Marthe variety and to 76.34 in the KWS Olof variety, while CuO NPs reduced genome stability to 53.33 in Marthe variety and in the KWS Olof variety to 68.81. The miR156a expression levels after Fe₃O₄ NPs treatment did not change in the Marthe variety, but increased in the KWS Olof variety, while CuO NPs treatment increased miRNA expression levels in the Marthe variety but decrease them in the KWS Olof variety. As NPs are able to influence miRNA expression and miRNAs can affect the plant resistance, obtained results suggest that tested NPs may alter plant resistance response to pathogens.

The size of nanoparticles (NPs) allows them to accumulate in plants, and they affect plant growth by altering the size of leaves and roots and affecting their photosynthetic reactions by altering the composition of proteins in the electron transport chain, chlorophyll biosynthesis, and carbohydrate synthesis reactions. Plants play an important role on Earth as nutrient producers in all trophic food webs by producing oxygen, absorbing carbon dioxide, and synthesizing edible carbohydrates during photosynthesis. In this study, Fe₃O₄ and ZnO NPs at various concentrations (0, 1, 2, and 4mg/l) were used to investigate the effect of NPs on plant morphological parameters and photosynthesis intensity, determining the amount of chlorophyll and the absorption of their light spectrum in common wheat (Triticum aestivum L.). Fe₃O₄ (25nm, 2mg/l, and 4mg/l) and ZnO (32nm, 4mg/l) significantly increased the leaf length of common wheat seedlings. However, Fe₃O₄ NPs (25nm, 1mg/l, and 4mg/l) significantly reduced light absorption at 645 and 663nm and the content of chlorophyll b, chlorophyll a, and total chlorophyll, but Fe₃O₄ (25nm, 2mg/l) significantly reduced the chlorophyll a content. In addition, ZnO NPs (32nm, 1mg/l) significantly increased the chlorophyll b content. This study has made a major contribution to understanding the effect of low concentrations of NPs on plant seedlings. Currently, NPs with high concentrations, starting at 10mg/l, have been analysed in other studies, but in the environment, NPs enter plants in low concentrations as dust or through water droplets. Therefore, it is important to determine the potential impact of small concentrations of NPs on crops that are important for agriculture.

Field-effect transistor-based biosensors (bio-FETs) are promising candidates for the rapid high-sensitivity and high-selectivity sensing of various analytes in healthcare, clinical diagnostics, and the food industry. However, bio-FETs still have several unresolved problems that hinder their technological transfer, such as electrical stability. Therefore, it is important to develop reliable, efficient devices and establish facile electrochemical characterization methods. In this work, we have fabricated a flexible biosensor based on an Al:ZnO thin-film transistor (TFT) gated through an aqueous electrolyte on a polyimide substrate. In addition, we demonstrated techniques for establishing the operating range of such devices. The Al:ZnO-based devices with a channel length/width ratio of 12.35 and a channel thickness of 50 nm were produced at room temperature via magnetron sputtering. These Al:ZnO-based devices exhibited high field-effect mobility (μ = 6.85 cm²/Vs) and threshold voltage (V_th = 654 mV), thus showing promise for application on temperature-sensitive substrates. X-ray photoelectron spectroscopy was used to verify the chemical composition of the deposited films, while the morphological aspects of the films were assessed using scanning electron and atomic force microscopies. The gate–channel electric capacitance of 40 nF/cm² was determined using electrochemical impedance spectroscopy, while the electrochemical window of the gate–channel system was determined as 1.8 V (from −0.6 V to +1.2 V) using cyclic voltammetry. A deionized water solution of 10 mer (CCC AAG GTC C) DNA aptamer (molar weight −2972.9 g/mol) in a concentration ranging from 1–1000 pM/μL was used as an analyte. An increase in aptamer concentration caused a proportional decrease in the TFT channel conductivity. The techniques demonstrated in this work can be applied to optimize the operating parameters of various semiconductor materials in order to create a universal detection platform for biosensing applications, such as multi-element FET sensor arrays based on various composition nanostructured films, which use advanced neural network signal processing.