Plants respond to adverse conditions by activating defense mechanisms that alter metabolism and impact agricultural crop yield. Organic mulching of Camellia oleifera leads to increased oil yield compared to control. In this study, multi-platform untargeted metabolomics and qRT-PCR were used to measure the effects of organic mulching on seed kernel metabolism. Metabolomics analysis revealed that tyrosine, tryptophan, and several fla-vonoids and polyphenol metabolites were significantly lower in the mulched treatment compared to the control, indicating lower stress levels with mulching. The qRT-PCR analysis showed that EAR, SAD, and CoHCD were up regulated by mulching, while CT, FAD7, FAD8, CoATS1, SQS, SQE, FATB, and beta-AS were down-regulated. Correlation network analysis was used to integrate data from this multi-omics investigation to analyze the relationships between differentially expressed genes, metabolites, and fruit and soil indicators concerning mulch treatment of C. oleifera. (c) 2021 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http:// creativecommons.org/licenses/by-nc-nd/4.0/).