Natural weak cohesive subgrade soils are considered low quality foundations for pavement structures that may lead to significant pavement distresses. Several solutions can be proposed to enhance pavement behavior under cyclic loads such as stabilizing clays with cement or lime, removing and replacing weak material, or increasing the base thickness to reduce the stresses on subgrade. Despite the effectiveness of using additives like lime and cement for subgrade stabilization, cement and lime are considered environmentally unfriendly owing to the generated emission during their production. Recently, several researchers have shown the potential of using biopolymers as alternative for traditional additives. This study investigates the feasibility of using sodium alginate biopolymer for treating weak cohesive subgrades particularly under repeated traffic loads for pavement construction applications. Two different soils (clay and silt), representing a typical range of cohesive soils in the delta region in Egypt, were investigated. The sodium alginate was added to the cohesive soils in the range of 0% to 6% using two different mixing methods (wet and dry). The experimental program included basic engineering tests, resilient modulus, and unconfined compressive strength tests, along with other chemical tests such as Fourier transform infrared spectroscopy (FTIR) and thermal gravimetric analysis (TGA). In addition, scanning electronic microscope (SEM) images were performed for both untreated and treated soils at different alginate contents. Results showed that the resilient modulus of the treated subgrade soils generally increased with the increase in sodium alginate content up to an optimum value of 2% and 4% for clayey and silty soils, respectively. This significant improvement was reached in the first 4 days of curing with continuous gain of strength up to 28 days. This increase in resilient modulus was found to be dependent on the soil type, alginate concentration, curing time, and treatment method. The microlevel tests, TGA, SEM, and FTIR, confirmed the presence of the cementation polymer due to sodium alginate that increased the bond and cross-linking between particles, and consequently an increase in the soil stiffness and strength occurred.

Integrating sustainability goals into the selection of suitable soil stabilization techniques is a global trend. Several bio-inspired and bio-mediated soil stabilization techniques have been recently investigated as sustainable alternatives for traditional techniques known for their high carbon footprint. Enzyme Induced Carbonate Precipitation (EICP) is an emerging bio-inspired soil stabilization technology that is based on the hydrolysis of urea to precipitate carbonates that cement sand particles. A life cycle assessment (LCA) study was conducted to compare the use of traditional soil stabilization using Portland cement (PC) with bio-cementation via EICP over a range of environmental impacts. The LCA results revealed that EICP soil treatment has nearly 90% less abiotic depletion potential and 3% less global warming potential compared to PC in soil stabilization. In contrast, EICP in soil stabilization has higher acidification and eutrophication potentials compared to PC due to byproducts during the hydrolysis process. The sensitivity analysis of EICP emissions showed that reducing and controlling the EICP process emissions and using waste non-fate milk has resulted in significantly fewer impacts compared to the EICP baseline scenario. Moreover, a comparative analysis was conducted between EICP, PC, and Microbial Induced Carbonate Precipitation (MICP) to study the effect of treated soil compressive strength on the LCA findings. The analysis suggested that EICP is potentially a better environmental option, in terms of its carbon footprint, at lower compressive strength of the treated soils.

Enzyme-induced carbonate precipitation (EICP) has been described as a bioinspired solution for ameliorating the mechanical properties of soil by precipitating calcium carbonate to bond sand particles. This study investigates the use of EICP utilizing a cost-effective jack bean meal as a source of enzyme to cement sand. Three different EICP cementing solutions were used to treat sand specimens in this study. The performance of biocemented sand was investigated under several key environmental conditions. These conditions include the effect of the EICP cementing solution concentration, curing time, temperature, wetting and drying cycles, exposure to sulfate contamination, and exposure to seawater. Increasing the concentration of the EICP solution facilitates dense calcite precipitation, which leads to higher soil strength. Scanning electron microscopy was used to observe the carbonate precipitation pattern. The images showed that increasing the curing temperature up to 40°C has led to more calcite and dense aragonite than vaterite, which means the enzyme has greater efficiency. A substantial increase in the unconfined compressive strength after one cycle of treatment with jack bean meal as the source of the enzyme was achieved, which tolerated the high salinity of the EICP cementing solution. The unconfined compressive strength of treated soils with a high-molarity EICP cementing solution was around 2.9 MPa at 5.6% ${\mathrm{CaCO}}_3$ . Finally, the EICP-treated sand specimens showed higher resistance against seawater and wetting/drying cycles of freshwater, whereas less resistance was observed for the case of immersion in a 5% sulfate solution.

The global construction industry consumes huge amounts of mined materials that are considered unsustainable for earth resources. In addition, Portland cement which is a key element in concrete and most construction materials is considered one of the main contributors to worldwide CO₂ emissions. On the other hand, natural cemented soil deposits are examples of sustainable structures that have survived decades of severe environmental conditions. Mimicking these natural biological systems provide an alternative to the current practices of construction materials production. Enzyme-induced carbonate precipitation (EICP) is a bio-inspired technique based on the precipitation of calcium carbonate for enhancing the geo-mechanical properties of soils. In this technique, calcium carbonate acts as a cementitious agent that binds the soil particles together at the points of contact, hence, increasing the strength and stiffness of treated soils, while relatively reducing the soil permeability and porosity. The achieved enhancements make EICP useful for applications such as ground improvement, construction materials, and erosion control over traditional binders. This paper presents a state-of-the-art review of EICP for ground improvement including the fundamental basics of EICP treatment. The paper also discusses the chemical and physical factors affecting the performance of EICP such as enzyme source, enzyme activity and solution constitutes. Moreover, the paper reviews the different methods and testing techniques used in the application of EICP for soil treatment. Furthermore, the paper compares EICP with other biomineralization techniques in terms of performance and applicability on ground improvement. Finally, the paper discusses the research gaps and existing challenges concerning the commercialization and large-scale implementation of the technology.