The study was carried out on individually and collectively housed growing rabbits from 34 to 63 days of age. Two experiments were conducted using three fat sources: Soybean oil (SBO), Soya Lecithin Oil (SLO), and Lard (L; Exp. 1), and SBO, Fish Oil (FO), and Palm kernel Oil (PKO; Exp. 2), added at two inclusion levels (1.5 and 4.0%). In both trials, 180 rabbits were housed in individual cages and additional 600 rabbits in collective cages from day 34 to 63. Animals fed with 4% dietary fat showed lower Daily Feed Intake (DFI) and Feed Conversion Ratio (FCR) than those fed with 1.5%, except in the individually housed animals in Exp. 1. In the collective housed group in Exp. 1, DFI was a 4.8% higher in animals fed with diets containing lard than those fed with SBO (p = 0.036). Lard inclusion also tended to reduce mortality (p = 0.067) by 60% and 25% compared with SBO and SLO diets, respectively. Mortality was the highest with the higher level of soya lecithin (14% vs. 1%, p < 0.01). A similar mortality rate was observed in the lowest level of SBO. In the grouped-housed animals in Exp. 2, a decrease of DFI (−12.4%), Bodyweight (BW) at 63 d (−4.8%), and Daily Weight Gain (DWG) (−7.8%) were observed with the inclusion of fish oil (p < 0.01) compared to other fat sources. Fish oil also tended to increase (p = 0.078) mortality (13.2%) compared with palm kernel oil (6.45%); similar results were found when animals were individually housed. The overall efficiency of N retention (NRE) increased with the highest level of fat in Exp. 1 (34.9 vs. 37.8%; p < 0.0001). It can be concluded that lard and palm kernel oil are alternative sources of fat due to the reduction of mortality. The inclusion of fish oil impaired animal productivity and increased mortality. An increment of the dietary fat level improved FCR and overall protein retention efficiency.

The aim of this study was to assess and validate, using independent data, the prediction equations obtained to estimate in vivo carcass composition using bioelectrical impedance analysis (BIA) to determine the nutrient retention and overall energy and nitrogen retention efficiencies of growing rabbits. Seventy-five rabbits grouped into five different ages (25, 35, 49, 63 and 77 days) were used in the study. A four-terminal body-composition analyzer was applied to obtain resistance (Rs, Ω) and reactance (Xc, Ω) values. All the animals were stunned and bled at each selected age, and the chilled carcasses were analyzed to determine water, fat, crude protein (CP), ash and gross energy (GE). Multiple linear regression analysis was conducted to determine the equations, using body weight, length and impedance data as independent variables. The coefficients of determination (R²) to estimate the content of water, protein, fat and ash in grams, and energy in Mega Jules(MJ), were: 0.99, 0.99, 0.95, 0.96 and 0.98, respectively, and the relative mean prediction errors (RMPE) were: 4.20, 5.48, 21.9, 9.10 and 6.77%, respectively. Carcass yield (%) estimation had values of 0.50 and 10.0 for R² and RMPE, respectively. When water content was expressed as a percentage, the R² and RMPE were 0.79 and 1.62%, respectively. When the protein, fat and ash were expressed as a percentage of dry matter (%DM) and the energy content as kJ/100 g DM, the R² values were 0.68, 0.76, 0.66 and 0.82, respectively, and the RMPEs were 3.22, 10.5, 5.82 and 2.54%, respectively. Energy Retention Efficiency was 20.4 ± 7.29%, 21.0 ± 4.18% and 20.8 ± 2.79% from 35 to 49, from 49 to 63 and from 35 to 63 d, respectively. Nitrogen Retention Efficiency was 46.9 ± 11.7%, 34.5 ± 7.32% and 39.1 ± 3.23% for the same periods. Energy was retained in body tissues for growth with an efficiency of approximately 52.5%, and the energy efficiency for protein and fat retention was 33.3 and 69.9%, respectively. This work shows that BIA is a non-invasive and good method to estimate in vivo carcass composition and to determine the nutrient retention of growing rabbits from 25 to 77 days of age.