Proactive silviculture treatments (e.g., thinning) may increase C sequestration contributing to climate change mitigation, although, there are still questions about this effect in Mediterranean pine forests. The aim of this research was to quantify the storage of biomass and soil organic carbon in Pinus forests along a climatic gradient from North to South of the Iberian Peninsula. Nine experimental Pinus spp trials were selected along a latitudinal gradient from the pre-Pyrenees to southern Spain. At each location, a homogeneous area was used as the operational scale, and three thinning intensity treatments: unthinned or control (C), intermediate thinning (LT, removal of 30–40% of the initial basal area) and heavy thinning (HT, removal of 50–60%) were conducted. Growth per unit area (e.g., expressed as basal area increment-BAI), biomass, and Soil Organic Carbon (SOC) were measured as well as three sets of environmental variables (climate, soil water availability and soil chemical and physical characteristics). One-way ANOVA and Structural Equation Modelling (SEM) were used to study the effect of thinning and environmental variables on C sequestration. Biomass and growth per unit area were higher in the control than in the thinning treatments, although differences were only significant for P. halepensis. Radial growth recovered after thinning in all species, but it was faster in the HT treatments. Soil organic carbon (SOC₁₀, 0–10 cm depth) was higher in the HT treatments for P. halepensis and P. sylvestris, but not for P. nigra. SEM showed that Pinus stands of the studied species were beneficed by HT thinning, recovering their growth quickly. The resulting model explained 72% of the variation in SOC₁₀ content, and 89% of the variation in silvicultural condition (basal area and density) after thinning. SOC₁₀ was better related to climate than to silvicultural treatments. On the other hand, soil chemical and physical characteristics did not show significant influence over SOC₁₀- Soil water availability was the latent variable with the highest influence over SOC₁₀. This work is a new contribution that shows the need for forest managers to integrate silviculture and C sequestration in Mediterranean pine plantations.

Increasing intensity and frequency of droughts are leading to forest dieback, growth decline and tree mortality worldwide. Reducing tree-to-tree competition for water resources is a primary goal for adaptive climate silviculture strategies, particularly in reforested areas with high planting density. Yet, we need better insights into the role of stand type (i.e., natural forests versus plantations) on the resilience of pine forests to droughts across varying time scales. In this study, we combined dendrochronological data and stable C (δ¹³C) and H (δ²H) isotopes measured in tree-ring wood as well as in specific wood chromatographically isolated compounds to investigate contrasting responses to drought of natural versus planted stands of two representative pine species, i.e., Pinus pinaster and Pinus nigra in southeastern Europe. Natural stands exhibited about two-fold increase in tree-ring growth in average (basal area at 20 years-BAI₂₀) as compared to planted stands. A response function analysis showed contrasting seasonal growth patterns for both species, which were related to monthly mean temperature and precipitation. Both stand type and species variables influenced growth resilience indices. Both pine species revealed contrasting resilience patterns among forest types; whereas planted stands seemed to be less sensitive to yearly droughts as determined by a higher recovery index (CRc) for P. pinaster, the contrary was found in the case of P. nigra. On the other hand, while resistance CR_T and resilience CR_S indices were higher for planted than natural forests in the case of P. pinaster, little differences were found for P. nigra. Beyond comparisons, carbon stable isotopes shed lights on the role of forest types in dry sites, being δ¹³C consistently lower in natural than in planted forests for both pine species (p < 0.05). We concluded that planted forest assimilated more carbon as per unit of water used than natural stands in response to droughts. Both δ¹³C and δ²H isotopic signals were positively correlated for both species for planted forests. However, a lack of correlation was evidenced for natural stands. Consistent with δ¹³C observations, δ²H concentrations in woody phenolic compounds (guaiacol and oleic acid) revealed contrasting patterns among forest types. This puts forward that δ²H concentrations in woody phenolic compounds (rather than in woody tree ring) accounts for other confounding factors in tree ring formation that can be associated with forest type. Our results highlight the value of stable isotope approaches versus conventional dendrochronological tools in drought studies and call for the consideration of forest type as an endogenous aspect defining the vulnerability of pine forests to climate.

Forests are key elements in mitigating the effects of climate change due to the fact of their carbon sequestration capacity. Forest management can be oriented to optimise the carbon sequestration capacity of forest stands, in line with other productive objectives and the generation of ecosystem services. This research aimed to determine whether thinning treatments have a positive influence on the growth patterns of some of the main Mediterranean pine species and, therefore, on their Carbon (C) fixation capacity, both in terms of living biomass and soil organic carbon. The results obtained show that C sequestration capacity (biomass and SOC) increased at higher thinning intensities due to the induced alterations in tree growth patterns. We observed almost a 1.5-fold increase in P. nigra and P. sylvestris, respectively, and over a two-fold increase in P. pinaster under heavy thinning treatments; SOC stocks were affected by the intensity of the thinning treatments. These results can contribute to improving silvicultural practices aimed at C sequestration in forest plantations located in dry areas of the Mediterranean.

Climate change is one of the environmental issues of global dominance and public opinion, becoming the greatest environmental challenge and of interest to researchers. In this context, planting trees on marginal agricultural land is considered a favourable measure to alleviate climate change, as they act as carbon sinks. Aerial laser scanning (ALS) data is an emerging technology for quantitative measures of C stocks. In this study, an estimation was made of the gains of C in biomass and soil in carob (Ceratonia siliqua L.) plantations established on agricultural land in southern Spain. The average above-ground biomass (AGB) corresponded to 85.5% of the total biomass (average 34.01 kg tree⁻¹), and the root biomass (BGB) was 14.5% (6.96 kg tree⁻¹), with a BGB/AGB ratio of 0.20. The total SOC stock in the top 20 cm of the soil (SOC-S₂₀) was 60.70 Mg C ha⁻¹ underneath the tree crown and 43.63 Mg C ha⁻¹ on the non-cover (implantation) area for the C. siliqua plantations. The allometric equations correlating the biomass fractions with the dbh and Ht as independent variables showed an adequate fit for the foliage (Wf, R²_adj = 0.70), whereas the fits were weaker for the rest of the fractions (R²_adj < 0.60). The individual trees were detected using colour orthophotography and the tree height was estimated from 140 crowns previously delineated using the 95th percentile ALS-metric. The precision of the adjusted models was verified by plotting the correlation between the LiDAR-predicted height (HL) and the field data (R²_adj = 0.80; RMSE = 0.53 m). Following the selection of the independent variable data, a linear regression model was selected for dbh estimation (R²_adj = 0.64), and a potential regression model was selected for the SOC (R²adj = 0.81). Using the segmentation process, a total of 8324 trees were outlined in the study area, with an average height of 3.81 m. The biomass C stock, comprising both above- and below-ground biomass, was 4.30 Mg C ha⁻¹ (50.67 kg tree⁻¹), and the SOC_20-S was 37.45 Mg C ha⁻¹. The carbon accumulation rate in the biomass was 1.94 kg C tree⁻¹ yr⁻¹ for the plantation period. The total C stock (W_-S and SOC_20-S) reached 41.75 Mg ha⁻¹ and a total of 4091.5 Mg C for the whole plantation. Gleaned from the synergy of tree cartography and these models, the distribution maps with foreseen values of average C stocks in the planted area illustrate a mosaic of C stock patterns in the carob tree plantation.