Shock wave-boundary layer interactions (SWBLIs) have a tremendous influence on the performance of hypersonic vehicles. For the numerical simulation of such engineering flows, Reynolds averaged Navier-Stokes (RANS) still occupies an irreplaceable role. However, parameters of turbulence models in RANS have substantial uncertainties, which impact the reliability of simulation results. Thus, the aim of the present study is to conduct an uncertainty analysis on parameters in the shear-stress transport (SST) turbulence model for the simulation of SWBLIs. In the current work, uncertainty quantification was performed first. A surrogate model was constructed by the non-intrusive polynomial chaos (NIPC) method to propagate uncertainties from model parameters to the quantities of interests (QoIs) and quantify them. In the subsequent sensitivity analysis, the key parameters were identified for such flow by calculating the Sobol index of each parameter for various QoIs. The results indicate that uncertainties of model parameters led to non-negligible uncertainties in those QoIs, particularly in skin friction and wall heat flux. The parameters α₁, σ_ω1, β₁ were identified as primary contributors through the sensitivity analysis. Moreover, the specific effects of the three parameters on the flow prediction were analyzed by changing the parameters’ values separately.