The aim of this study was to examine the relationship between motor proficiency and pedometer-determined physical activity in 5–6 year-old children. Participants (n = 232) were randomly recruited and assessed from 30 kindergartens in Northern Greece. Two trained researchers administered the measurements for the assessment of children’s motor proficiency by using the BOTMP-SF. Physical activity was assessed by OMRON pedometers. Significant relationships between BOTMP-SF standard score and steps (S), aerobic walking time (AWT) and aerobic steps (AS), (p < .05) were found. When motor proficiency was divided into quartiles to assess the distribution of the relationship between motor proficiency and pedometer- derived variables, significant associations were found for AWT, S and AS (p < .001). Young children with high levels of motor proficiency were more active in contrast to their peers with lower motor proficiency. The findings add to the growing body of literature that considers motor skills/abilities as important elements of physical activity participation. (Abbreviations: S-steps per day; AS-aerobic steps per day; AWT-aerobic walking time (minutesfiay⁻¹); BOTMP-SF-Bruininks-Oseretsky Test of Motor Proficiency-Short Form (standard score))