Background: Advanced nanobiotechnology provides safe and efficient drug delivery systems to deliver chemotherapy that targets cancer cells efficiently. Methods: A polymeric-magnetic nanocarrier was composed of a dextran (DEX) shell, a superparamagnetic iron oxide (SPION) core and was conjugated with folate (FA) to carry the anticancer drug vincristine (VNC) in Tera-1 testicular tumor cells. The molecular mechanisms by which apoptosis was induced were analyzed using flow cytometry and qPCR, which exhibited anticancer activity of nanoparticles (NPs). Results: This nanocarrier revealed a controlled release of VNC in citrate and phosphate buffer solutions that were maintained at pH 5.5 and pH 7.4, respectively. The Inhibitory concentration (IC50) values were greater than 5 mg/mL and displayed ten times higher cytotoxicity than the comparable free drug concentration. The Caspase-9 and P53 expressions were increased, whereas P21 and AKt1 decreased noticeably in the treated cells. The results point to the possible activation of apoptosis following treatment with NPs loaded with vincristine.

In this review, the theoretical and experimental aspects of ZnO nanostructures production using pulsed laser deposition techniques were presented. It reviewed the work principles of pulse laser deposition technique (PLD) method, physical procedures such as ablation, and plasma plume creation accompanying the deposition of pure and doped ZnO from target to substrate material. Many ways of deposition and elements that affecting on the properties of thin films like the temperature of substrate, laser fluence (laser energy density), pulse repetition rate, pressure of oxygen in chamber, time of deposition process and post growth annealing which modify the deposition active factors like nucleation, and crystallization.