The soil water characteristic curve and microstructure evolution of unsaturated expansive soil improved by microorganisms in Nanning, Guangxi were studied by means of filter paper method and scanning electron microscope imaging (SEM). Based on Fredlung & Xing model, the influence law of different cement content on the soil water characteristic curve of improved expansive soil is proved. According to the analysis of SEM test results, the influence mechanism of MICP method on the engineering characteristics of improved expansive soil is revealed. The results show that with the increase of cement content, the saturated water content and residual water content of the improved expansive soil gradually increased. At the same time, the water stability gradually increased while the air inlet value gradually decreased. The improved expansive soil changes from the superposition of flat particles and flake particles to the contact between spherical particles and flake particles, which indicates that the aggregate increases significantly. With the increase of the content of cement solution, the contact between particles tends to be smooth and the soil pores gradually tend to be evenly distributed. The particle size and microstructure of soil particles was changed and the connection between particles was enhanced in the improved expansive soil. Eventually the strength and water stability of expansive soil were improved. The conclusions above not only provide a theoretical basis for the in-depth study of engineering characteristics of unsaturated expansive soil improved by MICP method, but also offer theoretical evidence for perfecting engineering technology of expansive soil improved by MICP method.